Platelet
Platelet--rich plasma (PRP)
rich plasma (PRP)
increases healing process
increases healing process
of rats’ Achilles tendons
of rats’ Achilles tendons
KAUX JF, Drion P, Pascon F, Libertiaux V, Le Goff C, Gothot A,
Cescotto S, Defraigne JO, Rickert M, Crielaard JM
Biennial Congress for Sports Medicine and Sports Sciences 20 March 2010
of rats’ Achilles tendons
of rats’ Achilles tendons
• Tendon = tissue which does not heal easily.
• Recently, several studies, essentially in vitro and, more recently, a few in clinical practice, have demonstrated the positive effects of
Introduction
Introduction
have demonstrated the positive effects of
platelets on the healing process of different
tissues.
• A local injection of platelet–rich plasma (PRP), which releases many growth factors, has the potentiality to enhance the tendon healing process.
• The aim of our experiment was to ascertain
whether the use of platelet-rich plasma (PRP) was of interest for accelerating the healing
process of the Achilles tendon after surgical
Objective
Objective
process of the Achilles tendon after surgical induced lesion.
• All experimental procedures and protocols used in this investigation were reviewed and
approved by the Institutional Animal Care and
Use Committee of the University of Liège.
Methods
Methods
Use Committee of the University of Liège.
• 60 rats were divided into 2 groups:
– A: control (no injection).
– B: PRP injection.
• A 5mm defect was surgically induced in the rats’ Achilles tendons after resection of
Methods
Methods
• Rats of group B received a PRP injection in situ after the surgery.
• Afterwards, rats of both groups were placed in their cages without immobilization.
Methods
Methods
• After 5, 15 and 30 days, the traumatized
Achilles tendons of 10 rats of both groups
were removed and dissected during their healing process.
Methods
Methods
healing process.
• Immediately after sampling, tendons were submitted to a biomechanical tensile test up
to rupture, using a “Cryo-jaw”.
• Rats were then euthanized.
• Statistical analyses were made with an
Methods
Methods
Methods
Methods
Results
Results: force
: force
(N)(N)6 0 8 0 Time (days) Fo rc e ( N ) 5 10 15 20 25 30 2 0 4 0 6 0 Contrôle PRP
Results
Results: section
: section
(mm²)(mm²)S e c ti o n ( m m ²) 3 0 3 5 Time (days) S e c ti o n ( m m ²) 5 10 15 20 25 30 1 5 2 0 2 5 Control PRP
Results
Results: force/
: force/weight
weight
(N/100g)(N/100g)(N /1 0 0 g ) 0 .1 5 0 .2 0 Time (days) F o rc e /W e ig h t (N /1 0 0 g ) 5 10 15 20 25 30 0 .0 5 0 .1 0 Contrôle PRP
Results
Results:
: constraint
constraint
((MPaMPa))M P a ) 2.5 3 .0 Time (days) C o n s tr a in t (M P a 5 10 15 20 25 30 1 .0 1 .5 2 .0 Contrôle PRP
• We demonstrated that the force necessary to
induce tendon rupture during biomechanical
tensile test study was greater for tendons
which had been submitted to an injection of
Discussion
Discussion
which had been submitted to an injection of
PRP.
• These results were noticed and significant
• This experimentation has shown that PRP
injections could accelerate tendon healing process and increase the force needed to
rupture tendons in their healing process.
Conclusion
Conclusion
rupture tendons in their healing process.
• This “accelerating” process can be observed
Thank you for your attention.
jfkaux@chu.ulg.ac.be
Thanks to :
jfkaux@chu.ulg.ac.be
