Matériel et Méthodes

Dégradation de l’ADNccc 2.3

2 Matériel et Méthodes

Cultures cellulaires et infections virales

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Quantification des antigènes HBe et HBs

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Extraction ADN et ARN

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Quantification de l’ADN et l’ARN du VHB par qPCR Taqman

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Quantification de l’ADN et de l’ARN du VHB par la ddPCR

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3 Résultats

Validation des amorces pour la quantification des gènes viraux

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Validation de la quantification en duplex

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Validation de la sensibilité de la ddPCR par rapport à la qPCR

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Validation de la détection des évènements rares

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4 Discussion

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Dans le document De la détection de l'ADNccc par de nouvelles technologies à la preuve de concept de sa dégradation à visée thérapeutique dans des modèles d'infection par le virus de l'hépatite B (Page 138-158)

Dégradation de l’ADNccc 2.3

2 Matériel et Méthodes

3 Résultats

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4 Discussion

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