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Appendix S1 Sampling locations and number of haplotypes for the sampled populations

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Appendix S1 Sampling locations and number of haplotypes for the sampled populations

Species Country No. Population Origin* Collaborators, providing samples Vouchers** n 1 2 3 4 5 6

P. alba Austria 1 Vienna Danube_c NP Own sampling (Lexer et al. 2005, 2007) S 41 1 1 0 0 0 0

P. alba Austria 2 Northern Austria_alba_c NP Own sampling S 32 0 0 0 1 16 0

P. alba Croatia 3 Central Croatia NP Kajba D., Bogdan S. (University of Zagreb, HR) S 9 0 0 0 7 0 0

P. alba Croatia 4 Eastern Croatia_alba NP Kajba D., Bogdan S. (University of Zagreb, HR) S 5 0 0 0 0 0 0

P. alba Czech Republic 5 Czech Republic_alba_c CC Cvrčková H., Máchová P. (FGMRI, CZ) N 23 0 1 1 0 3 1

P. alba Greece 6 Crete NP Van Loo M. (RGB Kew, GB) S 7 0 0 0 0 0 0

P. alba Hungary 7 Eastern Hungary_c NP Bartha D. (NYME, HU) S 28 0 0 0 0 0 0

P. alba Hungary 8 Western Hungary_c NP Bartha D. (NYME, HU) S 8 0 0 0 0 2 0

P. alba Hungary 9 Southern Hungary_c NP Benke A. (ERTI Sarvar, HU) S 29 0 0 0 0 0 0

P. alba Italy 10 Northern Italy CC Castiglione S. (UniSa, I) N 4 0 0 0 0 0 0

P. alba Italy 11 Central Italy CC Castiglione S. (UniSa, I) N 6 0 0 0 0 2 0

P. alba Italy 12 Southern Italy CC Castiglione S. (UniSa, I) N 6 0 0 0 0 0 0

P. alba Romania 13 Central Romania NP Nica M.S., Ipati A. (ICAS, RO) S 27 0 0 0 0 0 0

P. alba Romania 14 Eastern Romania NP Nica M.S., Ipati A. (ICAS, RO) S 12 0 0 0 0 0 0

P. alba Romania 15 Western Romania NP Nica M.S., Ipati A. (ICAS, RO) S 17 0 0 0 0 0 0

P. alba Spain 16 Spain NP González-Martínez S. (INIA, E) N 8 0 0 0 0 0 0

P. alba Tunisia 17 Tunisia NP Own sampling S 6 0 0 0 0 0 0

P. alba Morocco 18 Morocco NP Own sampling S 3 0 0 0 0 0 0

P. tremula Austria 19 Northern Austria_trem_c NP Own sampling S 62 0 0 0 0 0 0

P. tremula Austria 20 Eastern Alps NP Own sampling S 16 0 0 0 0 0 0

P. tremula Austria 21 Viennese Forest_c NP Own sampling S 10 0 0 0 0 0 0

P. tremula Austria 22 Northern Alps NP Own sampling S 14 0 0 0 0 0 0

P. tremula Austria 23 Central Alps NP Own sampling S 10 0 0 0 0 0 0

P. tremula Croatia 24 Eastern Croatia_trem_c NP Kajba D., Bogdan S. (University of Zagreb, HR) S 10 0 0 0 0 0 0

P. tremula Czech Republic 25 Czech Republic_trem CC Cvrčková H., Máchová P. (FGMRI, CZ) N 22 0 0 0 0 0 0

P. tremula Sweden 26 Sweden NP Own sampling C 3 0 0 0 0 0 0

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No. Population 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 1 Vienna Danube_c 0 0 0 0 3 0 0 0 11 0 0 0 1 1 18 0 2 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 Northern Austria_alba_c 0 0 0 2 1 0 0 0 2 0 0 0 0 0 9 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 Central Croatia 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 Eastern Croatia_alba 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 5 Czech Republic_alba_c 0 0 1 1 2 0 0 0 0 0 2 0 0 1 5 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 Crete 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 7 Eastern Hungary_c 0 0 0 0 0 0 0 0 7 0 0 0 0 0 16 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 8 Western Hungary_c 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 Southern Hungary_c 0 0 0 0 0 0 0 0 4 0 0 1 0 0 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 Northern Italy 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 Central Italy 0 1 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 Southern Italy 1 1 0 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 Central Romania 0 0 0 0 0 0 0 0 4 1 0 0 0 0 19 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 14 Eastern Romania 0 0 0 0 0 0 0 0 1 0 0 0 0 1 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15 Western Romania 0 0 0 0 0 0 0 0 5 0 0 0 0 0 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16 Spain 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 1 0 1 1 0 0 0 0 17 Tunisia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 18 Morocco 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 19 Northern Austria_trem_c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4 0 0 1 0 0 0 0 30 0 20 Eastern Alps 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 3 0 21 Viennese Forest_c 0 0 0 0 2 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 22 Northern Alps 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 23 Central Alps 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 24 Eastern Croatia_trem_c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 25 Czech Republic_trem 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 6 3 26 Sweden 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0

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No. Population 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 1 Vienna Danube_c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 Northern Austria_alba_c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 Central Croatia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 Eastern Croatia_alba 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 Czech Republic_alba_c 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0

6 Crete 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

7 Eastern Hungary_c 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 8 Western Hungary_c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 Southern Hungary_c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 Northern Italy 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 Central Italy 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 Southern Italy 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 Central Romania 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 Eastern Romania 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15 Western Romania 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

16 Spain 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0

17 Tunisia 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2

18 Morocco 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

19 Northern Austria_trem_c 1 1 19 0 0 1 0 2 0 0 2 0 0 0 0 20 Eastern Alps 0 1 4 0 0 0 0 4 0 0 0 0 0 0 0 21 Viennese Forest_c 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 22 Northern Alps 0 1 3 0 0 0 2 3 0 0 2 0 0 0 0 23 Central Alps 1 0 0 0 6 0 0 1 0 1 0 0 0 0 0 24 Eastern Croatia_trem_c 0 0 4 0 0 0 0 1 0 0 1 0 2 0 0 25 Czech Republic_trem 0 0 4 4 0 0 0 0 1 0 0 0 0 0 0

26 Sweden 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0

Sample size (n). * Origin of samples (NP natural population, CC clone collection); ** Available voucher specimens (S silica-gel dried leaves, C

clone collection, N no material available)

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