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Reference
Assaying the regulatory potential of mammalian conserved non-coding sequences in human cells
ATTANASIO, Catia, et al.
Abstract
BACKGROUND: Conserved non-coding sequences in the human genome are approximately tenfold more abundant than known genes, and have been hypothesized to mark the locations of cis-regulatory elements. However, the global contribution of conserved non-coding sequences to the transcriptional regulation of human genes is currently unknown. Deeply conserved elements shared between humans and teleost fish predominantly flank genes active during morphogenesis and are enriched for positive transcriptional regulatory elements.
However, such deeply conserved elements account for
ATTANASIO, Catia, et al . Assaying the regulatory potential of mammalian conserved non-coding sequences in human cells. Genome Biology , 2008, vol. 9, no. 12, p. R168
DOI : 10.1186/gb-2008-9-12-r168 PMID : 19055709
Available at:
http://archive-ouverte.unige.ch/unige:1281
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SUPPLEMENTARY FIG.S1.Vectors used in enhancerand promoterstudies.
(a)CNCSs were cloned upstream ofa minimalTK promoterin a non-directionalorientation,16 CNCSs were accumulated in both orientation.
(b)pRL-SV40 was transfected along with the pTAL-luc experimentalvectorto controlfortransfection efficiency in the enhancer/repressorassay.
(c)putative promoterCNCSs (single orclustered)were cloned upstream ofthe luciferase reportergene in theirnative orientation,13 were obtained in the reverse orientation too.
(d)pREP7 luc was transfected along with the pREP4 luc,as an internalcontrolfortransfection efficiency.
