Effect of feed restriction on outcomes of swine influenza virus infection in pigs pre-infected with Mycoplasam Hyopneumoniae

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Effect of feed restriction on outcomes of swine influenza virus infection in pigs pre-infected with Mycoplasam

Hyopneumoniae

Céline Deblanc, Nathalie Le Floc’H, Elodie Merlot, Jean-Noël Sialelli, Fabien Vautrin, Rolland Cariolet, Gaelle Simon

To cite this version:

Céline Deblanc, Nathalie Le Floc’H, Elodie Merlot, Jean-Noël Sialelli, Fabien Vautrin, et al.. Effect of

feed restriction on outcomes of swine influenza virus infection in pigs pre-infected with Mycoplasam

Hyopneumoniae. 2nd International Symposium on Neglected Influenza Viruses (ISIRV), Mar 2013,

Dublin, Ireland. 2013, Final programme and book of abstracts. �hal-02749140�

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International Symposium on Neglected Influenza Viruses www.isirv.org

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more widely studied to determine if the data observed in this study represent the normal situation or an exceptional one. A wider study and collection of more epidemiological data will be performed to understand if influenza A virus circulation in wild population could represent a serious threat for domestic pigs or also to human.

POSTER 13

Effect Of Feed Restriction On Outcomes Of Swine Influenza Virus Infection In Pigs Pre-Infected With Mycoplasma Hyopneumoniae

C. Deblanc⁽¹⁾, N. Le floc’h⁽²⁾⁽³⁾, E. Merlot⁽²⁾⁽³⁾,JN. Sialleli⁽⁴⁾, f.

Vautrin⁽⁴⁾, R. Cariolet⁽⁵⁾, G. Simon⁽¹⁾

(1) Anses, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, Ploufragan, France, (2) INRA, UMR1348 Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage, Saint-Gilles, France, (3) Agrocampus Ouest, UMR1348 PEGASE, Rennes, France, (4) FARM’APRO, Lamballe, France, (5) Anses, Ploufragan-Plouzané Laboratory, Production of decontaminated pigs and testing, Ploufragan, France

European avian-like swine H1N1 was identified as one major pathogen of the porcine respiratory disease complex (PRDC) together with Mycoplasma hyopneumoniae (Mhp), and experimental pre-infection of pigs with Mhp was shown to increase the severity of a subsequent infection with H1N1. In order to evaluate novel control strategies towards such a multifactorial disease, we studied the impact of a moderate feed restriction on the ability of Mhp/H1N1 co-inoculated animals to resist the infection, as efficiency of inflammatory responses might be influenced by the nutritional status of the animal. Two groups of 8 SPF pigs were intra-tracheally inoculated with Mhp and H1N1 21 days apart. One group was fed ad libitum whereas the other one was applied a 40% feed restriction one week before H1N1 infection. Two similar mock- inoculated groups of 4 pigs each were included. All pigs were fitted with a jugular catheter. Three days post-H1N1 infection, the same amount of a standardized meal was given to all animals and kinetics of blood samples were performed during 4 hours for measuring plasma postprandial nutrient concentrations. Pigs were slaughtered 7 days post-H1N1 infection. Clinical signs were observed throughout the study and pathogens were detected in nasal swabs and lung tissues. Feed restriction had no effect on pathogen excretion and dissemination in infected host or on pulmonary lesions. However, feed- restricted pigs presented a shorter hyperthermia as well as a positive mean weight gain over the 3 first days following H1N1 infection as compared to animals fed ad libitum which lost weight during that period. Both infection and feed restriction modified postprandial kinetics of glucose and amino acid concentrations showing dramatic changes in nutrient metabolism. Our results indicated that feeding practices could be a strategy to prepare animals to overcome an influenza infection, especially in a PRDC context.

POSTER 14

Organization Of The French National Surveillance Network For Influenza In Pigs

G. Simon¹,S. Hervé¹, N. Rose¹, P. Amar², P. Le Coz³, I.

Tourette⁴, C. Locatelli⁵, C. Marcé⁶

1Anses, French National Reference Laboratory for Swine Influenza, ²Coop de France, ³SNGTV, ⁴GDS France,

5ADILVA, ⁶DGAl

Swine influenza viruses (SIV) are of important concerns for both animal and human health. As it was reminded by OIE following the emergence of the swine-origin H1N1 pandemic virus in 2009, expansion of influenza virus identification in pig herds is necessary to provide new data about potential changes in the epidemiology of enzootic SIVs, as well as about adaptation and circulation of novel zoonotic viruses in that species. In France, a national network associating governmental organizations and private partners from the pig production was set up in April 2011 in order to reinforce the surveillance of influenza viruses in domestic pigs. The objectives are to describe in time and space i) the nature of circulating SIVs, ii) their respective frequencies in the pig population and iii) the epidemiological data linked to SIV infections. The network is driven at the national level by the Ministry of Agriculture (DGAl) and Coop de France, an organization representative of agricultural cooperatives. In each of the 22 administrative regions, a local organizer is suggested by professional organizations and named by the Regional Food Authority (SRAL). The regional organizer recruits voluntary veterinarians and provides them with sampling kits composed of 3 nasal swabs, an accompanying document and a stamped envelope. The surveillance is passive and the vet has to sample 3 pigs with hyperthermia from one given unit, during an acute flu outbreak. He fills in the accompanying document, providing epidemiological information at both individual and herd levels, but herds are kept anonymous to other partners. The vet posts the kit to one of the closest approved veterinary laboratory. Indeed, 12 local laboratories were designated by DGAl for the detection of Influenza A virus genome in pig biological samples by RT- PCR methods validated by the National Reference Laboratory (Anses-NRL). Sampling kits and detection analyses are financially supported locally by SRAL.

Positive samples are sent to Anses-NRL for virus identification, as well as all accompanying documents for epidemiological analyses. During the first year of this network, the surveillance has been implemented in 12 regions and 171 outbreaks were investigated, leading to the detection of 108 positive herds and the identification of 85 SIVs.

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