HAL Id: hal-03123921
https://hal.univ-reims.fr/hal-03123921
Submitted on 9 Feb 2021
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Bacillus subtilisPTA-271 mediated-ISR in Arabidopsis requires a primed SA-dependent camalexin response
upon pathogen challenge
Ngoc Nguyen, Patricia Trotel-Aziz, Sandra Villaume, Fanja Rabenoelina, Christophe Clément, Fabienne Baillieul, Aziz Aziz
To cite this version:
Ngoc Nguyen, Patricia Trotel-Aziz, Sandra Villaume, Fanja Rabenoelina, Christophe Clément, et al..
Bacillus subtilisPTA-271 mediated-ISR in Arabidopsis requires a primed SA-dependent camalexin
response upon pathogen challenge. PlantBioProtech, 2019, AGADIR, Morocco. �hal-03123921�
B. subtilis PTA-271
SA
NPR1
Camalexin
ISR against B. cinerea
ISR against Pst DC3000
NahG sid2
Proposed model of camalexin priming by PTA-271 upon infection withB. cinereaandPst DC3000
npr1
pad3
Bacillus subtilis PTA-271 mediated-ISR in Arabidopsis requires a primed SA-dependent camalexin response upon pathogen
challenge
Ngoc Huu Nguyen, Patricia Trotel-Aziz, Sandra Villaume, Fanja Rabenoelina, Christophe Clément, Fabienne Baillieul, Aziz Aziz Research Unit EA 4707 RIBP, SFR Condorcet FR-CNRS 3417, University of Reims, UFR Sciences, Campus Moulin de la Housse, 51100 Reims, France
Bacillus subtilisPTA-271 has been shown to induce ISR against the necrotrophBotrytis cinerea(Verhagen et al., 2011) and the hemibiotrophNeofusicoccum parvum(Trotel-Aziz et al., 2019) in grapevine. However, the mechanisms by which the bacterium activates the host’s immune response underlying ISR remain to be elucidated. In this study, we examined whether the extent of priming state could contribute to the PTA-271-induced ISR inArabidopsis, by focusing on the camalexin response andCYP71A12gene expression afterB. cinereaandPst DC3000infection.Our data provide evidence that PTA-271 triggers ISR against both pathogens through priming camalexin synthesis in SA- and NPR1-dependent manner. This response was further compromised in pad3 mutant after pathogen infection, highlighting that camalexin plays a prominent role inB. subtilis-ISR against both necrotroph and hemibiotroph.
RESULTS
PTA-271 triggers ISR against B. cinerea andPst DC3000 inArabidopsis
PTA-271 CTRL
+ B. cinerea + Pst DC3000
b
a
0 1 2 3 4 5
CTRL PTA-271
Necrosis diameter (mm) b
a
0 20 40 60 80 100
CTRL PTA-271
% diseased leaves
Roots of wild-type plants Col-0 were inoculated with PTA-271 for 2 weeks, then leaves were infected withB. cinereaandPstDC3000. ISR was determined at 4 days post infection.
PTA-271 significantly reduces the symptoms and disease incidence caused byB.
cinereaandPstDC3000.
PTA-271 primes camalexin synthesis upon pathogen challenge Priming of camalexin production andCYP71A12gene expressions were analysed by UPLC and RT-qPCR, respectively. The samples were selected in different time of the pathogens infection.
aa b
c d
e
f
0 200 400 600
0 24 48 72 96
Camalexin (ng/g FW) CTRL
PTA-271
a a a
b c
b d
c
0 20 40 60 80 100
0 24 48 72 96
+ B. cinerea + Pst DC3000
a
b b
a b
c
0 20 40 60 80 100
0 12 24
Relative expression
Hours-post infection CYP71A12
CTRL PTA-271
a
b b
a
c c
0 1 2 3 4 5 6
0 12 24
Hour-post infection CYP71A12
PTA-271 primes camalexin accumulation and CYP71A12 gene expression in response to the pathogens
Stronger level of camalexin andCYP71A12expression are primed in B. cinerea-infected leaves
Loss of camalexin synthesis in pad3mutant compromises PTA-271-ISR against both B. cinerea and PstDC3000
b a
c b
0 1 2 3 4 5 6
CTRL PTA-271 CTRL PTA-271
Col-0 WT pad3
necrosis diameter (mm)
b
a
b b
0 20 40 60 80 100
CTRL PTA-271 CTRL PTA-271
Col-0 WT pad3
Diseased leaves (%)
Priming of camalexin accumulation contributes to PTA-271-ISR againstB. cinereaand PstDC3000
SA and NPR1 are required for priming camalexin after pathogen infection
+ B. cinerea + Pst DC3000
CONCLUSION
PTA-271 induces ISR against necrotrophic and hemi-biotrophic pathogens by priming camalexin synthesis throughCYP71A12expression
PTA-271-primed camalexin is dependent on SA and NPR1 pathway
Camalexin accumulation is dependent on SA and NPR1, but not on JA and ET uponB. cinereaandPstDC3000 challenge.
References
1. Trotel-Aziz, P., Abou-Mansour, E., Courteaux, B., Rabenoelina, F., Clément, C., Fontaine, F., et al.
(2019). Front. Plant Sci. 10
2. Verhagen BWM, Trotel-Aziz P, Jeandet P, Baillieul F, Aziz A (2011). Phytopathology 101:768–777
b a a a a b
b
a b a c c
e
c e
c e
d f
c d
c
f f
0 100 200 300 400 500 600
Col-0 WT sid2 NahG npr1 ein2.1 jar1.2 Col-0 WT sid2 NahG npr1 ein2.1 jar1.2
CTRL PTA-271
Camalexin (ng/g FW)
+B. cinerea 0hpi
96hpi
b a a a a b b
a c
a
c c
e
d c
d
b e
f
e e e
f f
0 10 20 30 40 50 60 70 80
Col-0 WT sid2 NahG npr1 ein2.1 jar1.2 Col-0 WT sid2 NahG npr1 ein2.1 jar1.2
CTRL PTA-271
Camalexin (ng/g FW)
+ Pst DC3000
