These preliminary results indicate that eBSV could be an adapted marker to precise Musa phylogeography
O
ur aim is to study the integration context of such infectious integrants for Banana streak virus amongthe diversity of the banana B genome in order to retrace the evolutionary BSV story and understand their impact on host and virus evolution in terms of cost/benefit. To answer this question, we propose to characterize infectious integrants (eBSV) among a representative sampling of Musa balbisiana diversity and to hypothesize a contribution of eBSV towards plant virus resistance through an RNA interfering mechanism versus a maintain of the virus through interspecific crosses. © Nat ha lie Le Gall
T
he nuclear genome of several plants isinvaded by numerous viral sequences. These integrations correspond to accidental events mainly resulting from illegitimate recombination of DNA viruses belonging to the family Caulimoviridae with plant DNA whereas integration into the host genome is not required for viral replication. These
integrations are for the most part defective as a result of pseudogenisation driven by the host genome evolution. Conversely, some other integrations named infectious, could release a functional viral genome following activating stresses.
eBSGFV
A strong correlation exists between southern blots and PCR results PKW hallmark is strongly conserved among the diploid genotypes Positive selection of the infectious allele among the diversity of the banana B genome
Pierre-Olivier Duroy
1, Xavier Perrier
2, Matthieu Chabannes
1, Nathalie Laboureau
1,
Marie-Line Iskra-Caruana
11 CIRAD/UMR BGPI , 2 UMR AGAP, F-34398 Montpellier cedex 5 pierre-olivier.duroy@cirad.fr
Sampling of B genome diversity
20 BB banana plants only
no geographic information 69 Inter specific hybrids AB, ABB, AAB
from the 2 areas of interspecific hybridization origin13.3 kb
eBSGFV- infectious
eBSGFV-non infectious 15.6 kb
eBSV Goldfinger in PKW - allelic integration
ORF1
ORF2
ORF3
IR
BSV
Dendrogramme from 69 banana plant samples calculated with NJ algorithm and based on 498 Dart markers
AAB Popoulou ABB East AAB Pome AAB others AAB Pome AAB Plantain ABB west banksii0620 Agutay Long Tavoy Maia Oa Pahang IDN 110 Fougamou 1
Kluai Namwa Khom Pelipita
Burro Cemsa Dole
Blue Java
Pisang Kepok Bung Daru
Bengani Auko
Saba
Figue Pomme Géante Tay Tia
Foconah Prata ana
Lady Finger (Nelson) Yangambi no 2
Mai'a popo'ulu moa
Popoulou Tigua Tangtang Boung Fu Gamaha Corne 1 Kelong Mekintu Orishele Luba P. Kapas
Pisang Raja Bulu Pisang Radjah Kluai Roi Wi Kunaimp Chuoi Mat Pisang Nangka Pisang Ceylan Pisang Slendang Muracho Porp Dimaemamosi Safet Velchi Kunnan
Ney Poovan/Figue Pomme(Ekona)
Balbisiana (10852) Balbisiana I63-080 Balbisiana HDN 211 Balbisiana LBA-342 Balbisiana0545 Balbisiana0626 Balbisiana1016
Butuhan, intermediate apex
Cameroun Eti Kehel Honduras Lal Velchi Singapuri Tani Pisang Batu Pisang Klutuk
Pisang Klutuk Wulung Los banos IITA
Montpellier IITA I63 IITA 10852 IITA Chinois 1Chinois 2 Chinois 3 Butuhan
Lep Chang Kut Butohan IITA
Eti kehel IITA
coccinea laterita0627 ornata textilis1072 mannii H. Wendl velutina basjoo
Not Named Mainz
Dendrogramme from 84 banana plant samples calculated with NJ algorithm based on eBSOLV hallmarks
Dendrogramme from 78 banana plant samples calculated with NJ algorithm based on eBSGFV hallmarks
AAB others AAB Pome ABB West ABB East AAB Popoulou AAB Plantain
(Delanghe et al, ERA 2009)
Geographic distribution of the main inter specific triploids
banksii0620 Agutay Long Tavoy Maia Oa Pahang IDN 110 Fougamou 1
Kluai Namwa Khom Pelipita
Burro Cemsa
Dole
Blue Java
Pisang Kepok Bung
Daru Auko
Saba
Figue Pomme Géante
Tay Tia
Foconah
Prata ana
Lady Finger (Nelson)
Yangambi no 2
Mai'a popo'ulu moa
Popoulou Tigua Gamaha Corne 1 Kelong Mekintu Orishele P. Kapas
Pisang Raja Bulu
Pisang Radjah Kluai Roi Wi Kunaimp Chuoi Mat Pisang Nangka Pisang Ceylan Muracho Porp Bluggoe Roulo Safet Velchi Kunnan
Ney Poovan/Figue Pomme(Ekona)
Balbisiana (10852) Balbisiana I63-080 Balbisiana HDN 211 Balbisiana LBA-342 Balbisiana0545 Balbisiana0626 Balbisiana1016
Butuhan, intermediate apex
Cameroun Eti Kehel Honduras Lal Velchi Tani Pisang Batu Pisang Klutuk
Pisang Klutuk Wulung Los banos IITA
Montpellier IITA I63 IITA 10852 IITA Chinois 1 Chinois 2 Chinois 3 Butuhan
Lep Chang Kut
Butohan IITA
Eti kehel IITA
coccinea laterita0627 ornata textilis1072 mannii H. Wendl velutina basjoo
Not Named Mainz
AAB Plantain AAB Popoulou ABB East ABB west AAB others AAB Pome AAA
ABB AAB Out Group
BB ABB west ABB East AAB Plantain AAB others AAB Popoulou AAB Pome BB AA Agutay Auko Balbisiana (10852) Balbisiana HDN 211 Balbisiana I63-080 Balbisiana LBA-342 Balbisiana0545 Balbisiana0626 Balbisiana1016 banksii0620 basjoo Bengani Blue Java Boung Fu Burro Cemsa
Butuhan, intermediate apex Cameroun Chuoi Mat Corne 1 Daru Dimaemamosi Dole Eti Kehel
Figue Pomme Géante
Foconah Fougamou 1 Gamaha Honduras IDN 110 Kelong Mekintu Kluai Roi Wi Kunaimp Kunnan
Lady Finger (Nelson)
laterita0627
Long Tavoy
Luba
Maia Oa
Mai'a popo'ulu moa
mannii H. Wendl Muracho
Not Named Mainz Orishele
ornata
Pahang
Pelipita
Pisang Ceylan
Pisang Kepok Bung
Pisang Nangka
Pisang Radjah
Pisang Raja Bulu Pisang Slendang Popoulou Prata ana Saba Safet Velchi Singapuri Tangtang Tay Tia textilis1072 Tigua velutina Yangambi no 2
Pisang Klutuk Wulung PKW (BB) as a model
23.2 kb
eBSOlV-non infectious
eBSOlV-infectious 22.9 kb
eBSV Obino l’Ewai in PKW - allelic integration
Specific hallmark for each eBSV integration in PKW
PCR markers
Southern blot fingerprints
eBSOLV
A faint correlation exists between southern blots and PCR results PKW hallmark is restricted to PKW clade
Integration loci are highly conserved
A large area of differentiation with a lot of changes and mutations
resulting on identification of novel alleles in the west hybridization area Positive selection of the infectious allele
eBSGFV
eBSOLV
Infectious allele
Non Infectious allele Non eBSGFV allele
Infectious allele
Non Infectious allele Non eBSGFV allele
