• Aucun résultat trouvé

Derivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks


Academic year: 2021

Partager "Derivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks"

En savoir plus ( Page)

Texte intégral


Derivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks


Most of the human embryonic stem cell (hESC) lines have been derived from the inner mass of blastocyst stage embryos, some from 8-cell stage morula (Streclchenko et al., 2004) and some from isolated blastomeres from morulae (Chung et al., 2008).

In a very recent abstract, a Belgian team (Van de Velde et al., 2008) elegantly established an embryonic stem cell line from a single blastomere isolated from a 4-cell stage embryo (from 1 out of 12 embryos). This team had a unique opportunity to establish good quality embryos from donated oocytes and sperm. By deriving a line from one blastomere, they gave evidence for the totipotency of single cells at 4-cell stage in human embryo. The result-ing hESC line was, however, chromosomally abnormal with a deletion in chromosome 18 and a duplication in chromosome 7.

In Switzerland, the embryo research law is very strict, and obtaining good quality embryos for hESC derivation is vir-tually impossible, even though the law allows hESC deri-vation and culture. Our team recently derived a hESC line (Ch-ES1) from a frozen-thawed 4-cell stage embryo, in which only one blastomere survived thawing. The surviving cell grew on human foreskin feeders (Hovatta et al., 2003) and formed a hESC-like colony which has since then been passaged and cultured as a hESC line for over a year (Feki et al., 2008). This line proved to be chromosomally very abnormal, with triploidies and several deletions and dupli-cations. It forms malignant teratocarcinoma-like tumors when injected into SCID mice.

The Belgian team has now an excellent opportunity to show how often such lines are abnormal, a type of study still very dif-ficult in many other countries. It is not new that poor quality embryos often have chromosomal abnormalities (Hardarson et al., 2003), but on the other hand, we do not know yet how much culture conditions affect the chromosomes of early lines, even though culture adaptation is well known (Draper et al., 2004).

In this light, the interpretation of many media that deri-vation of lines from single blastomeres is ethically a more acceptable method of derivation of hESC lines than destroy-ing whole embryos cannot be supported by the scientific com-munity. It appears that sometimes even the scientists themselves provoke such ideas (Chung et al., 2008). The Belgian team has just been revealing very important biologi-cal mechanisms, but some media changed even their message to that direction. Also in Switzerland, we have to make clear that we now have an important tool to solve some problems in cancer development, but it does not offer solutions for any ethical questions in hECS derivation. The fact that hESC lines can be obtained from cells without destroying the embryo, or from cells of a ‘dead’ embryo, does not make such lines ethically more acceptable. Abnormality issues should not be underestimated.


Chung Y, Klimanskaya I, Becker S, Li T, Maserati M, Lu SJ, Zdravkovic T, Ilic D, Genbacev O, Fisher S et al. Human embryonic stem cell lines generated without embryo destruction. Cell Stem Cell 2008;2:113 – 117. Draper JS, Smith K, Gokhale P, Moore HD, Maltby E, Johnson J, Meisner L,

Zwaka TP, Thomson JA, Andrews PW. Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat Biotechnol 2004;22:53 – 54

Feki A, Bosman A, Dubuisson JB, Irion O, Dahoun S, Pelte MF, Hovatta O, Jaconi M. Derivation of a human embryonic stem cell line (ESC) from a single blastomere of an arrested stage four embryo. Swiss Medial Weekly 2008;138:540– 550.

Hardarson T, Caisander G, Sjogren A, Hanson C, Hamberger L, Lundin K. A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts. Hum Reprod 2003;18:399 – 407.

Hovatta O, Mikkola M, Gertow K, Stro¨mberg AM, Inzunza J, Hreinsson J, Anda¨ng L, Ahrlund-Richter L. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 2003;18:1404 – 1409.

Streclchenko N, Verslinsky O, Kukharenko V, Verlisnky J. Morula-derived human embryonic stem cell lines. Reprod Biomed Online 2004;9:623 – 629. Van de Velde H, Geens M, Mateiyel I, Spits C, Sermon K, De Rycke M, Van der Elst J, Devroey P, Tournaey H, Liebaers I. A human embryonic stem cell line derived from a single blastomere of a 4-cell stage embryo. ESHRE 2008, 6 – 9 July, Barcelona. Hum Reprod Abstract O-274.

A. Feki1,2, O. Hovatta2,3,5 and M. Jaconi4

1Laboratory of Stem Cell Research, Geneva University

Hospitals, Geneva, Switzerland;

2Department of Obstetrics and Gynecology, Geneva University

Hospitals, Geneva, Switzerland;

3Division of Obstetrics and Gynecology, Department of

Clinical Sciences, Karolinska Institutet, Stockholm, Sweden and

4Department of Pathology and Immunology, Faculty of

Medicine, Geneva University, Switzerland


Correspondence address. E-mail: outi.hovatta@ki.se doi:10.1093/humrep/den369 Advance Access publication on October 14, 2008

Impact of hyperglycemia on early embryo development and embryopathy: in vitro experiments

using a mouse model Sir,

Fraser et al. (2007) identify the media they used in their work as mKSOM and KSOM, and characterize them as a ‘synthetic oviductal medium enriched with potassium’. We wish to point out that his characterization is erroneous. A medium called SOM was developed by Lawitts and Biggers (1991) using the experimental strategy called ‘sequential simplex optimization’ (hence SOM). SOM medium was not designed to imitate the composition of oviductal secretions. It was developed to overcome the 2-cell block which, at the time, interfered with many studies on the developmental biology of the mouse embryo. KSOM was later modified by raising the potassium concentration in SOM (Lawitts and Biggers, 1993) based on



Documents relatifs

Pour ce module, estimé à 30 heures de travail étudiant, nous avons choisi d'indexer des ressources qui correspondent à ce que nous pourrions appeler un sous-chapitre dans

ENSO signals, together with multi-decadal variability in their impact as identified through seasonal values of the Interdecadal Pacific Oscillation (IPO) index, are shown to

The HBV­NP model is calibrated for soluble reactive phosphorus, total phosphorus and dissolved inorganic nitrogen, also with data from the lake outflow, but with explained

Significant difference was found in the incidence of the random group compared with the orthogonal and deviant. Total cell count and ICM/TE ratio in bovine blastocysts according to

Un merci tout particulier au groupe «Le Narcisse» pour sa précieuse collaboration, ainsi qu'aux enfants des membres de la Société des Amis fribourgeois de Montreux pour

SHiC and M ap IO strategies. Thus, we first compare the jitter obtained with the existing mapping strategies on the case study A illustrated in section IV. We then explain the impact

185 Figure 115 Comparaison des résistivités en fonction des coefficients de diffusion apparents des ions chlorures obtenus sur bétons par migration et ce pour différents

(This is the case, for example, for two agents located initially in symmetric, and thus indistinguishable, positions at the endpoints of a 3-node path.) However, we will overcome

If the reflections are not delayed too much they reinforce the direct sound and serve a useful purpose; in fact this sort of acoustical reinforcement is essential if the

L’accès à ce site Web et l’utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D’UTILISER CE SITE WEB.

Patients referred from the French outpatient genetic clinics for molecular screening to document colonic adenomatous polyposis from 1993 and 2008 were systematically screened

Contour plots show CD1a (x-axis) and CD27 (y-axis) expression before agonist stimulation and after 1, 2 or 3 rounds of agonist stimulation for TCR-transduced cells in co-cultures

Preliminary Results of Multiple Epiphyseal Drilling and Autologous Bone Marrow Implantation for Osteonecrosis of the Femoral Head Secondary to Sickle Cell Disease

clusters represent five different PEtn transferase families as follows; blue, lipid A-specific PEtn transferases, including EptA; green, PEtn transferases specific for the 6 position

Total protein content (mg/ml) of osteogenic cells derived from human alveolar bone cultured on dense titanium (Ti) and porous Ti for 7 and 10 days.. (a) indicates

In the present study, we followed the morphology as well as the expression of the CD3, CD56, IL-17, TNF ␣ and INF ␥ markers in the PER-VAS T-cell line, derived from an aggressive

Molecular and functional characterization of rabbit embryonic stem cell lines.. Pierre Osteil, Suzy Markossian, Murielle Godet, Thierry Joly, Pierre Savatier,

The analysis was performed on 20 different inducible clones of our mES cell bank (7 effective and 13 silent genes) and on parental ES cells (EB3) at the beginning of this study on

A teratocarcinoma-like human embryonic stem cell (hESC) line and four hESC lines reveal potentially oncogenic genomic changes.. HOVATTA, Outi,

Upon further addition of LIF and bFGF to single cell culture medium, Oct-4 expression increased over time, and Oct-4 mRNA levels were found to be significantly higher in single

We herein show that the expression profile for KRT5/KRT14 along the differentiation period is similar to what was described for the keratinocyte differentiation of several human

Differentiation increases the permissiveness of cES cells for GaHV-2 Initial tests performed to evaluate the level of replication of GaHV-2 in pluripotent cES cells showed that