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how to do the rapid test to detect G6PD deficiency

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1013365 A

01135-1 KENDALL

ALCOHOL PREP

Store at 4-30oC(3 G6PD ASSAY BUFFER

G6PD

Rapid G6PD Deficiency Whole Blood Test | Professional Use Only REF G0221

30oC

4oC IVD i 1

START/STOP

0:00 00

G6PD Test Kit Instructions

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REF G0221

IFU-G0221-CEF / Rev.C

Test kit instructions

Timer

Test kit

Alcohol

swab Pipette Lancet Assay buffer

Gloves

16. Dispose of the gloves and alcohol swab in the infectious waste

container. If not available, then put them, along with

desiccant and packaging in a general waste

container.

8. Wipe away the first drop of blood with a sterile gauze or cotton wool, then discard in the infectious waste bin (or sharps box if this is not available).

Gently squeeze the finger to allow a drop of blood to form. Use a pipette to collect the drop of blood. Gently hold the pipette (indicated by

the blue arrow) and touch the drop of blood.

Sterile gauze

No colour change, or a very faint purple colour means the patient haS G6PD deficiency.

2. Put on the gloves. Use new gloves for each patient.

1. Check the expiry date on the test

package. 3. Open the package and remove: 4. Write the patient’s name on the test.

a. Test

5. Open the alcohol swab. Grasp the 4 th finger on the patient’s left hand.

Clean the finger with the alcohol swab.

Allow the finger to dry before pricking.

9. Use the pipette to put the correct volume of blood into the sample hole marked “S.” Cover and press the hole on the pipette to release the blood.

Test package

12. Wait 10 minutes after adding buffer.

13. Read test results.

(NOTE: Do Not read the test before 10 minutes after adding the buffer.

You may get FaLSE results.)

15. How to read the test results:

A distinct purple colour means the patient DOES NOT have G6PD deficiency.

G6PD DEFICIENT G6PD NORMaL INVaLID RESULT

Invalid results are either where there is no blood flow or incomplete blood flow across the results window at the time of reading.

If you get an INVALID result, repeat the test using a NEW unopened test package and a NEW unopened lancet.

NOTE: Each test can be used ONLY ONE TIME.

Do not try to use the test more than once.

READ THESE INSTRUCTIONS CAREFULLY BEFORE YOU BEGIN.

Collect:

a. NEW unopened test package b. NEW unopened alcohol swab c. NEW unopened lancet

d. NEW pair of disposable gloves e. Buffer

f. Sterile gauze or cotton wool g. Timer

h. Sharps box i. Pencil or pen

how t o d o the r apid t est to d etect G6PD d eficiency

7. Discard the lancet in the sharps box immediately after pricking the finger. Do not set the lancet down before discarding it.

17. Record the test results in your health worker register. Dispose of cassette in non-sharps waste container.

10. Discard the pipette in the sharps box or the infectious waste bin.

Expiry date

11. Add two drops of buffer into the round hole marked “a.”

Alcohol swab

Buffer Timer

Pencil

Lancet

6. Open the lancet. Do not allow the tip of the lancet to touch anything before pricking the patient’s finger.

Prick patient’s finger to get a drop of blood.

031918

WHO/CDS/GMP/2018.16 © WHO 2018. Some rights reserved. This work is available under the CC BY-NC-SA 3.0 IGO licence.

b. Desiccant sachet (verify colour indicator, if applicable)

Disposable gloves

Adapted from malaria RDT generic job aids produced by World Health

Organization, United States Agency for International Development, TDR,

Foundation for Innovative New Diagnostics, and URC

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