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WAVE binds Ena/VASP for enhanced Arp2/3 complex-based actin assembly.

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FIGURE 2:  VASP affects lamellipodial actin dynamics during ventral enclosure. (a) Imaging of Lifeact-GFP expressed  exclusively in epidermal cells during ventral enclosure for wild-type embryos and for embryos lacking VASP
FIGURE 3:  WAVE recruits VASP for enhanced actin-based motility in vivo. (a) Lifeact-GFP imaging (WT WAVE and  ΔPRD-WAVE) or Lifeact-mCherry imaging (ΔEVH1-VASP) of ventral enclosure in embryos with reintroduced wild-type  WAVE or with mutant WAVE and VASP
FIGURE 4:  VASP’s F-actin and profilin/G-actin binding activities are important for its effect on  WAVE-based motility
FIGURE 5:  Teamwork between the Arp2/3 complex and VASP via mutual binding to WAVE.

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