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Enzymatic Synthesis of Dithiolopyrrolone Antibiotics
Using Cell-Free Extract of Saccharothrix algeriensis
NRRL B-24137 and Biochemical Characterization of
Two Pyrrothine N-Acyltransferases in This Extract.
Safwan Saker, Ziade Almousa Almaksour, Anne-Claire Chorin, Ahmed
Lebrihi, Florence Mathieu
To cite this version:
Safwan Saker, Ziade Almousa Almaksour, Anne-Claire Chorin, Ahmed Lebrihi, Florence Mathieu.
Enzymatic Synthesis of Dithiolopyrrolone Antibiotics Using Cell-Free Extract of Saccharothrix
al-geriensis NRRL B-24137 and Biochemical Characterization of Two Pyrrothine N-Acyltransferases in
This Extract.. Journal of microbiology and biotechnology, The Korean Society for Microbiology and
Biotechnology, 2014, 24 (1), pp.26-35. �10.4014/jmb.1309.09035�. �hal-01335779�
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OULOUSE
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uverte (
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Eprints ID : 10818
To link to this article : DOI : 10.4014/jmb.1309.09035
URL :
http://dx.doi.org/10.4014/jmb.1309.09035
To cite this version :
Saker, Safwan and Almousa Almaksour, Ziade
and Chorin, Anne-Claire and Lebrihi, Ahmed and Mathieu, Florence
Enzymatic Synthesis of Dithiolopyrrolone Antibiotics Using
Cell-Free Extract of Saccharothrix algeriensis NRRL B-24137 and
Biochemical Characterization of Two Pyrrothine
N-Acyltransferases in This Extract. (2014) Journal of microbiology
and biotechnology, vol. 24 (n° 1). pp. 26-35. ISSN
1738-8872
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Enzymatic Synthesis of Dithiolopyrrolone Antibiotics Using Cell-Free
Extract of
Saccharothrix algeriensis NRRL B-24137 and Biochemical
Characterization of Two Pyrrothine N-Acyltransferases in This Extract
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Introduction
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Materials and Methods
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The semi-synthetic medium (SSM) [2] containing 15 g/l of D (+)
glucose (Acros) was used. Sa. algeriensis precultures were prepared in 250 ml Erlenmeyer flasks containing 50 ml of culture medium inoculated with spores (2 × 107
CFU/ml). Cultures grown in 500 ml Erlenmeyer flasks containing 100 ml of SSM were inoculated with 5 ml of 48 h aged preculture. The culture was incubated at 30°C at 240 rpm for 72 h on a rotary shaker (New Brunswick Scientific Company, NJ, USA). .0//23"00*45,"-&,*!"06-"-,'#(*7."%$0*45,"-&,8
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Results
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Discussion
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Acknowledgments
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