MIAE : a collection dedicated to soil microbial diversity and environment (INRA, Dijon, France)

HAL Id: hal-02824206

https://hal.inrae.fr/hal-02824206

Submitted on 6 Jun 2020

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MIAE : a collection dedicated to soil microbial diversity and environment (INRA, Dijon, France)

Cécile Héraud, Thérèse Corberand, Nadine Gautheron, Cécile Revellin, Veronique Edel-Hermann, Philippe Lemanceau, Christian Steinberg

To cite this version:

Cécile Héraud, Thérèse Corberand, Nadine Gautheron, Cécile Revellin, Veronique Edel-Hermann, et al.. MIAE : a collection dedicated to soil microbial diversity and environment (INRA, Dijon, France).

12. International Conference on Culture Collections (ICCC12), Sep 2010, Santa Catarina, Brazil. 1

p., 2010. �hal-02824206�

To ensure a reliable system in the acquisition, preservation, identification and distribution of its strains, the MIAE collection must meet different requirements :

HISTORICAL & AIMS

Keywords : soilborne microorganisms, conservation, identification, distribution, quality management

ENGAGED IN QUALITY MANAGEMENT

ISO9001:2008

Quality management systems — Requirements

NF S 96-900

Quality of biological resource centres (BRCs) — Management system of a BRC and quality of biological resources of human and microbiol origin

International Center for Microbial Resources

created by INRA in order to centralize its microbial collections and to ensure their long-term preservation

2 years certification

1 year certification

Integration request

Cécile HERAUD*, Thérèse CORBERAND, Nadine GAUTHERON, Cécile REVELLIN, Véronique EDEL-HERMANN, Philippe LEMANCEAU, Christian STEINBERG

INRA-National Institute of Agronomical Research/University of Burgundy UMR 1229 - Microbiology of Soil and Environment 17 rue Sully, F-21000 Dijon, Dijon, France.

*E-mail : cecile.heraud@dijon.inra.fr Website : http://www2.dijon.inra.fr/umrmse/

MIAE collection, UMR1229 MSE

INRA^©/C. HERAUD

World Federation for Culture Collections - 12th International Conference on Culture Collections (ICCC12) - Florianópolis, Santa Catarina, Brazil - 26 September - 01 October 2010 AREA 4 - Session Microbial Resource Centers : Preservation, Quality management, Legal and safety issues / POSTER 24

REFLECTION OF THE FUNCTIONAL DIVERSITY OF SOIL MICROORGANISMS

http://www.biopack.dk

Nitrogen nutrition of plants

(Rhizobium)

Plant growth

(Pseudomonas, Fusarium)

Degradation of pesticides

(bacteria, fungi)

Plant pathogens

(fungi)

Bio-indiicators of soil health

(yeasts)

Biocontrol

(bacteria, fungi)

CONSERVATION OF THE MICROBIAL DIVERSITY OF SOILS

continuous deposits (~500 strains/year) 15 300 strains

fungi

bacteria

others Rhizoctonia

yeasts others

INRA©/A. ROBIN

Pseudomonas

Rhizobium

INRA^©/R. BRUNEAU INRA©/N. HAMINI

Fusarium

VISIBLE & ACCESSIBLE

Open collection accessible to scientific community via a web page http://www2.dijon.inra.fr/umrmse/spip.php?rubrique21

ª inventory

ª distribution/use protocol (Material Transfer Agreement form)

~250 delivered strains/year ª deposit protocol

IMPLICATED IN CURRENT PROJECTS

ª HOW TO CONSERVE ) MICROBIAL RESOURCES

Most of the strains are preserved at -80°C with glycerol in duplicate.

Rhizoctonia and oomycota (Pythium, Phytophthora) are preserved on agar slants at room temperature. Long-term preservation protocols at 4°C are under evaluation.

) RELATED DATA

Creation of a unique relational database (PostgreSQL

) that will be used for the administration of the collection and the on- line catalogue.

ª HOW TO IDENTIFY

) MORPHOLOGICAL METHODS

Use of macro- and microscopic identification keys ) CHEMOTYPICAL METHODS

Detection of mycotoxin using HPLC-DAD__

) MOLECULAR METHODS

Use of specific-primers, DNA sequences in accordance with «barcode»

standards (for example see Fiers et al., Poster 28 Area 2)

INRA^©/J. LEPLAT Macroconidies of MIAE00376 Fusarium graminearum

48 genera 13 genera

TCTB/ZEA profile of MIAE00376 (Fusarium graminearum)

The MIAE (Microorganisms of Interest for Agriculture and Environment) structure is holding over 15 300 soil-borne microbial strains (bacteria and fungi). These microorganisms have been isolated for over 30 years of scientific investigations related to soil functioning. Up to 2008, each microbial resource was preserved by the scientist involved in isolation, leading sometimes to orphan collections.

The creation of a unique and common collection aiming at guaranteeing the preservation of these microbial resources requires a High Quality standard approach. There is no equivalent structure all around the world. It includes sets of reference strains (Fusarium sp., Rhizoctonia sp., Rhizobium sp., Pseudomonas sp.) permitting scientists to characterize in a taxonomic and functional way their own strains.

MIAE: a Collection Dedicated to Soil Microbial Diversity and Environment (INRA, Dijon, France)

To better know, conserve and use soil microbial resources