HAL Id: hal-03107529
https://hal.archives-ouvertes.fr/hal-03107529v2
Submitted on 12 Jan 2021
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asexual cell cycle division in Toxoplasma gondii
Asma Khelifa, Cecilia Guillen Sanchez, Kevin Lesage, Ludovic Huot, Thomas Mouveaux, Pierre Péricard, Nicolas Barois, Hélène Touzet, Guillemette
Marot, Emmanuel Roger, et al.
To cite this version:
Asma Khelifa, Cecilia Guillen Sanchez, Kevin Lesage, Ludovic Huot, Thomas Mouveaux, et al..
TgAP2IX-5 is a key transcriptional regulator of the asexual cell cycle division in Toxoplasma gondii.
Nature Communications, Nature Publishing Group, 2021, 12, pp.116. �10.1038/s41467-020-20216-x�.
�hal-03107529v2�
ARTICLE
TgAP2IX-5 is a key transcriptional regulator of the asexual cell cycle division in Toxoplasma gondii
Asma S. Khelifa 1 , Cecilia Guillen Sanchez 1 , Kevin M. Lesage 1 , Ludovic Huot 1 , Thomas Mouveaux 1 , Pierre Pericard 2 , Nicolas Barois 1 , Helene Touzet 2,3 , Guillemette Marot 2,4 , Emmanuel Roger 1 &
Mathieu Gissot 1 ✉
Apicomplexan parasites have evolved efficient and distinctive strategies for intracellular replication where the timing of emergence of the daughter cells (budding) is a decisive element. However, the molecular mechanisms that provide the proper timing of parasite budding remain unknown. Using Toxoplasma gondii as a model Apicomplexan, we identi fi ed a master regulator that controls the timing of the budding process. We show that an ApiAP2 transcription factor, TgAP2IX-5, controls cell cycle events downstream of centrosome duplication. TgAP2IX-5 binds to the promoter of hundreds of genes and controls the acti- vation of the budding-speci fi c cell cycle expression program. TgAP2IX-5 regulates the expression of speci fi c transcription factors that are necessary for the completion of the budding cycle. Moreover, TgAP2IX-5 acts as a limiting factor that ensures that asexual proliferation continues by promoting the inhibition of the differentiation pathway. Therefore, TgAP2IX-5 is a master regulator that controls both cell cycle and developmental pathways.
https://doi.org/10.1038/s41467-020-20216-x OPEN
1
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, F-59000 Lille, France.
2Univ.
Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UMS 2014-PLBS, bilille, F-59000 Lille, France.
3Univ. Lille, CNRS, Centrale Lille, UMR 9189- CRIStAL-Centre de Recherche en Informatique Signal et Automatique de Lille, F-59000 Lille, France.
4Univ. Lille, Inria, CHU Lille, ULR 2694-METRICS:
Evaluation des technologies de santé et des pratiques médicales, F-59000 Lille, France. ✉ email: mathieu.gissot@pasteur-lille.fr
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