Pseudomonas cannabina pv. cannabina pv. nov., and Pseudomonas cannabina pv. alisalensis (Cintas Koike and Bull, 2000) comb. nov., are members of the emended species Pseudomonas cannabina (ex Šutič & Dowson 1959) Gardan, Shafik, Belouin, Brosch, Grimon

Supplemental Table 1. Phenotypic characteristics that are variable among strains of Pseudomonas cannabina or Pseudomonas syringae pv.

alisalensis.

species P. cannabina emend. P. syringae

genomospecies G9 G3 G1

Pathovars P. cannabina pv. cannabina P. cannabina pv. alisalensis pv. tomato pv. maculicola pv. syringae

Phenotypic characters^a

CFBP 2341^T

CFBP 1619

CFBP 1631

LMG 5540

LMG 5650

CFBP 6866^Pt

CFBP 6867

CFBP 6869

CFBP 6873

CFBP 6875

CFBP

1637 CFBP 2212^Pt CFBP 1657^Pt CFBP 1392^T

Ice nucleation

+ NT

+ ^{NT NT} + + + + + ^NT - - +

Hydrolysis of

Gelatin

- - - NT NT - - - - - - - - +

Tween 80

+ + + NT NT + + + + + + + + -

Acid from

Erythritol

- - - NT NT - - - - - - - - +

Mannitol

- - - NT NT + + + + + + + + +

Utilization of

DL-Lactate

- - - ^{NT NT} - - - - - - - - +

Assimilation of

i-erythritol

- - - - - - - - - - - - - +

meso-tartrate

- - - - - - - - - - - + + -

trans-aconitate

- - - - - - - - - - - + + +

DL-lactate

- - - - - - - - - - - - - +

DL-glycerate

- - - - - - - - - - - + + +

DL- -β

hydroxybutyrate

- - - - - - - - - - - - - +

Sucrose

- - - - - + + + + + + + + +

D(+) arabitol

- - - - - + + + + + + + + +

Myo inositol

- - - - - + + + + + + + + +

D-mannitol

- - - - - + + + + + + + + +

D(-) tartrate

- - - - - + + + + + + + + -

D(+) malate

- - - + - + + + + + + + + -

D-galacturonate

- - - + - + + + + + + + + -

Protocatechuate

- - - - - + + + + + + + + -

(-) quinate

- - - - - + + + + + + + + -

D-alanine

- - - - + + + + + + + + + +

Alpha-

ketoglutarate

- - - - - + + + + + + + + +

Putrescine

- - - - - - + + + + + - - -

D(+) trehalose

+ + + - - - - - - - + - - -

Esculin

+ - + - + - - - - - - + + +

L(+) arabinose

- + - - - + + + + + + + + +

D(+) xylose

- + - + - + + + + + + + + +

D-tagatose

- + - - - - - - - - - - - -

D-sorbitol

- - - - - + + - + - - + + +

D-lyxose

- - - - - + + + + - - - + -

cis-aconitate

- - - - - - - + + + + + + +

Trigonelline

- - - - - - - + + + + + + +

Betain

- + - - + + + + + + + + + +

DL-alpha- amino-n-

butyrate

- + - - - + + + + + + + + +

Caprate

- - - - - + + - - - - + + +

Caprylate

- - - - - + + - - - - + + -

Succinate

+ - + + + + + + + + + + + +

Fumarate

+ - + + + + + + + + + + + +

Glutarate

- - - - - + + - + - + - - -

Malonate

+ - + + - + + - + + + + + -

L-tyrosine

- - - - - - - - - - + - + -

p-

hydroxybenzoate

- - - - - + - + - + + + + -

Proprionate

- - - - - - - - + + + - + +

Polypectate pH 5

+ + - ^{NT NT} - - - - - - - + -

Polypectate pH 8

- - - ^{NT NT} - - - - - + - - -

a

genomonspecies as delineated by Gardan et al. [19].

b

Test for which all strains tested were positive included: D-glucose, D-fructose, D-galactose, D -Mannose, D-ribose, glycerol, D-saccharate, mucate, L-malate, citrate, D-glucuronate, D-gluconate, L-aspartate, L-glutamate, L-proline, L-alanine, L-serine, acid from saccharose. In addition, all strains were negative for the assimilation of Pectine, Nitrate reduction, utilization of L(+) tartrate, and DNAse activity as well as for the 44 Biotype characters not listed here.

c

NT = Not tested.

Supplemental Figure 1. Symptoms of diseases caused by Pseudomonas cannabina or Pseudomonas syringae pv. alisalensis on Canabis sativa, and Brassica rapa subsp. rapa.

Pseudomonas cannabina CFBP 2341

Pseudomonas syringae pv. alisalensis CFBP 6866

Marijuana, Canabis

sativa

Broccoli raab, Brassica rapa subsp.

rapa

Oat, Avena sativa

In multiple experiments with multiple replications, broccoli raab and oat leaves inoculated

with P. cannabina occasionally had some symptoms. However, P. cannabina has not been

reisolated from surface disinfested symptomatic tissue.

Supplemental Figure 2.

Dendrogram of phenotypic characteristics based on Unweighted Pair Group Method with Averages. Distance = 1 – Jaccard coefficient.

CFBP, Collection Française de Bactéries Phytopathogènes, Angers, France. Genomospecies 1-9 of the « P. syringae » cluster (G1-G9).

P. cannabina pv. alisalensis CFBP 6866^Pt (G9)

P. cannabina pv. cannabina CFBP 2341^T (G9) P. syringae pv. maculicola CFBP 1657^Pt (G3) P. cannabina pv. alisalensis CFBP 6867 (G9)

0.1 0.2 0.3 0.4 0.5

0

0.5370

P. cannabina pv. alisalensis CFBP 6873 (G9) P. cannabina pv. alisalensis CFBP 6875(G9) P. cannabina pv. alisalensis CFBP 6869 (G9) P. cannabina pv. alisalensis CFBP 1637 (G9)

P. syringae pv. tomato CFBP 2212^Pt (G3)

P. syringae pv. syringae CFBP 1392^T (G1) P. cannabina pv. cannabina CFBP 1619 (G9)

P. cannabina pv. cannabina CFBP 1631 (G9)