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HAL Id: hal-02264835

https://hal-normandie-univ.archives-ouvertes.fr/hal-02264835

Submitted on 7 Aug 2019

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New Insights into HIV-1 Group O Diversity

Marie Leoz, Florence Damond, Jude Kfutwah, Jean-Christophe Plantier, French Res-O Network

To cite this version:

Marie Leoz, Florence Damond, Jude Kfutwah, Jean-Christophe Plantier, French Res-O Network. New Insights into HIV-1 Group O Diversity. CROI, Mar 2013, Atlanta, United States. �hal-02264835�

(2)

Abstract #: I-112 Category: Molecular Epidemiology and HIV/SIV Evolution

New Insights into HIV-1 Group O Diversity

Marie Leoz 1 , Florence Damond 2 , Jude Kfutwah 3 , Jean-Christophe Plantier 1 and the French RES-O Network

1

Laboratoire associé au CNR du VIH, hôpital Ch.Nicolle, CHU de Rouen, France,

2

AP-HP, Groupe Hospitalier Bichat Claude Bernard, Service de Virologie, Paris, France,

3

Centre Pasteur du Cameroun, Yaounde, Cameroon

METHODS

OBJECTIVE : To explore HIV-O diversity through the largest series of HIV-O sequences, from Cameroon (HIV-O diagnosis and follow-up in the Centre Pasteur du Cameroun) and France (RES-O surveillance network).

CONTACT

Jean-Christophe PLANTIER

Laboratoire de Virologie CHU Charles Nicolle

1, rue Germont 76000 Rouen

[email protected]

RESULTS BACKGROUND

At least twelve cross-species transmissions of SIV (Simian Immunodeficiency Viruses) to humans led to the emergence of 4 HIV type 1 (M, O, N, P) and 8 HIV type 2 (A-H) groups.

HIV-O remained endemic to Cameroon with limited exportation to some closely related countries.

Previous nomenclature proposals, based on few sequences available then, highlighted a broad genetic diversity and opposed strains from a major clade (A or I) to the others.

Roques et al., 2002 ( env gp41) Yamaguchi et al., 2002 ( env gp160)

HIV type 1 group M (HIV-M) became

pandemic and

strain exportations followed by founder effects led to the definition of nine subtypes.

HIV-M HIV-O

HIV-M

HIV-O

Archer et al., 2007 ( env gp160)

987 bp 513 bp

Sequenced in 241 patient samples

Sequenced in 231 patient samples

112 + 125 104 + 123

Nomenclature comparison (env gp41)

297 sequences (231+58 from HIV database)

Yamaguchi et al.

Phylogeographic distribution (pol pr-RT)

269 sequences (241+28 from HIV database)

Roques et al.

A1

A2

A3

C B

Classification of 297 HIV-O strains according to the previous nomenclatures

Roques

A ND B C ND

228 (76,8%) 7 (2,35%) 14 (4,7%) 17 (5,7%) 31 (10,4%)

A1 A2 A3

186 (62,6%) 12 (4,0%) 30 (10,1%)

Yamaguchi

I IV ND V II III ND

186 (62,6%) 12 (4,0%) 30 (10,1%) 7 (2,35%) 14 (4,7%) 17 (5,7%) 31 (10,4%)

Ia Ib Iu

27 (9,1%)

53 17,8%)

106 (35,7%)

CONCLUSIONS

• The tree topology is less structured than the one of HIV-M : fewer clusters due to local continuous diversification

• Opposition between a short branch-length major clade and several long branch minor ones : epidemiologic / phylodynamic significance or sampling bias? Distinct phenotypic properties between those clades? (e.g. Y181C mutation in the RT)

• Need for a consensus nomenclature

• No significant clustering of strains sampled in France indicating continuous importations rather than a local spread

Iu Ia

Ib IV

V III II

HIV-O are circulating in France due to close history links with Cameroon (136 patients identified).

The distribution of the strains sampled in France and in Cameroon are different, but there is no evidence of a significant French cluster, unlike for the strains sampled in Gabon, Senegal or Spain.

Ib

Iu

Ia

IV

V

III II

Sampled in :

Maximum Likelihood Trees inferred from these sequences plus

those available in the HIV database and

classified in the nomenclature systems proposed

before

Cameroon (n=140) France (n=105)

Gabon (n=4) Senegal (n=3) Spain (n=4)

Current nomenclature systems are partially redundant, partially discordant and fail to describe the whole HIV-O diversity.

Acknowledgements: Centre Pasteur du Cameroun and Institut de Veille Sanitaire for fundings

Références

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