Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la
première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n’arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca.
Questions? Contact the NRC Publications Archive team at
PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information.
https://publications-cnrc.canada.ca/fra/droits
L’accès à ce site Web et l’utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D’UTILISER CE SITE WEB.
READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE. https://nrc-publications.canada.ca/eng/copyright
NRC Publications Archive Record / Notice des Archives des publications du CNRC :
https://nrc-publications.canada.ca/eng/view/object/?id=63d9426c-4a7c-40e5-838e-8d8826ba2bfa
https://publications-cnrc.canada.ca/fra/voir/objet/?id=63d9426c-4a7c-40e5-838e-8d8826ba2bfa
NRC Publications Archive
Archives des publications du CNRC
Access and use of this website and the material on it are subject to the Terms and Conditions set forth at
Process development for extraction and purification of bioproducts
PROCESS DEVELOPMENT FOR
EXTRACTION AND PURIFICATION OF
BIOPRODUCTS
Ashwani Kumar
AMTEC, Kuala Lumpur
May 2009
Lei Xu
NRCan’s-CBIN
Karen Lamb
NRC-NBP &
Linda Layton
AAFC-ABIP
Pinghai Shao
Nikhil Kawachale
• Introduction – importance of biomass
• NRC’s National Bioproduct Program
• Research Projects –two recent examples
– Nutraceuticals – process development
– Recovery and purification of chemicals in biochemical
conversion of biomass components
• Canada’s biomass harvest
143
Mt Carbon / year
• Available residue/waste
60
Mt Carbon / year
• Energy content
1.5-2.2 EJ/year (E=10
18
)
• Represents 18-
27% of Canada’s annual energy needs
• Challenge - biomass conversion
– Renewable energy and products comparable to fossil
fuel-derived products,
– Profitable,
– Significant environmental and social benefits
Primary
– unprocessed - direct combustion
Secondary- extraction of value-added compounds.
processed
– charcoal, gases, liquid fuel
Thermo-chemical
–
pyrolysis, gasification
– synthesis
gas, bio-oil,
source of chemicals
Physico-chemical
– seed oil, conversion to
biodiesel
Bio-chemical
– biological processes – ethanol,
biogas,
chemicals (e.g. diols)
Focus - Thermo-chemical & Physico-chemical
Biomass
Bio-oil
Gases
Bio-refinery concept
Nutraceuticals
Basic chemicals
Phenols,
Flavoring agents,
Polymers
CO, H
2
, CH
4
Fuel
Syngas, hydrogen
Feed pre-treatment
Reaction Engineering,
Fractionation
Material development
Characterization
Process development
Pilot scale studies
Focus
– Biochemical Conversion
Biomass
Bio-refinery Concept
Cellulose
Hemi-cellulose
Pentoses
Lignins
Butanediol,
ethanol,
acetoine
Recovery and purification
of butanediol
• Bio-oil – stabilization and characterization
• Extraction and purification from biomass extracts – nutraceuticals
• Recovery and purification of butanediol – membrane process
• Biogas Upgrading – new membranes
• CO
2
Capture
– new membranes
• CFD Studies – membrane test cells
• Skim Soymilk – whey contained 1/3 of the total isoflavones
(Daidzein and genistein as glucoside and esters)
• Recovery of isoflavones using a membrane-based process
– (UF/diafiltration, RO)
• Soy-based isoflavones –estrogenic activity – health concerns
• Alternate source – red clover flowers – biochanin A, formononetin
(Genistiein and daidzein as glucosides)
J. Am Oil Chemists Soc., 81 (2004) 91-96.
Food Research International 37 (2004) 867
–874.
• Dried - organically grown red clover flowers
• Water extraction under varying temperature and pH
• Adsorption of isoflavones on polyvinylprrolidone
• Elution by ethanol and recovery
Effects of Temperature & pH
0 10 20 30 40 50 0 30 60 90 120 150 180 210 Is of la vone c onc e nt ra ti on (( μ g/ g)Extraction time (min)
22 (room temperature) 60 70 80 90 0 10 20 30 40 50 60 70 80 0 30 60 90 120 150 180 210 Is of la von e con ce nt ra tion ( ( μg /g )
Extraction time (min)
3.0 9.0
9.5 10.0
10.5
Adsorption - PVP
0 10 20 30 40 50 60 70 80 90 100 5% 10% 15% 20% 30% Is o fl a v o n e s a d so rb e d (a s % o f e xt ra ct e d a mo u n t)PVP to red clover ratio (w/w)
0 10 20 30 40 50 60 70 80 90 100 1 2 3 4 5 Is of la v one s a ds or be d (a s % of e xt ra ct e d a m ount )
Ethanol-Water - Extraction
0 10 20 30 40 50 60 70 0 50 100 150 200 Is o fl a v o n e co n ce n tr a ti o n ( μ g /m L )Extraction time (minutes)
0% 30% 40% 50% 100% 0 10 20 30 40 50 60 70 80 90 100 0 20 40 60 80 100 E x tr a ct a b il it y ( %) Ethanol concentration (%, v/v)
