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Characterisation of sunflower-21 (SF21) genes expressed in pollen and pistil of Senecio squalidus (Asteraceae) and their relationship with other members of the SF21 gene family

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Academic year: 2022

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Fig. S1 a, Full-length ORSF21 gene sequences amplified from Senecio squalidus genomic DNA.

The S-genotype of the individual is indicated above each lane. b, Restriction digestion of cloned S. squalidus genomic ORSF21 sequences with the restriction endonucleases HindIII and RsaI.

The genotypes used were: Lanes 1–4, S1S2: lanes 5-8, S1S3; lanes 9-12, S1S6: lanes 13-15, S1S4. The sequences were cloned from PCR products shown in a. In the case of genotypes S1S2 and S1S4, the smaller PCR products (white arrows) were cloned

Fig. S2 a, 3D model of the SF21 protein, based on structural similarity to the hydrolase protein family. b, functionally similar sites between ORSF21 and a hydrolase signalling protein, RsbQ (Kaneko et al. 2005). Model produced using Phyre software (Kelley et al. 2000).

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Marker S1S2 S1S2 S1S3 S1S6 S1S4

4 kb

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 M 3 kb

0.5 kb

a

b

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a b

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