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Phosphorylation/Dephosphorylation of the Repressor MDBP-2-H1 Selectively Affects the Level of Transcription from a Methylated Promoter in Vitro

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Academic year: 2021

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Figure 1. (A) Construction of the template for in vitro transcription was carried out as described in methods section
Figure 2 shows the effect of increasing concentrations of methylated and non-methylated templates on the transcription
Figure 4. (A) Gel shift competition assay of MDBP-2-H1–DNA complex with increasing concentrations of methylated or unmethylated oligonucleotide (double-stranded oligonucleotide containing the MDBP-2-H1 binding site at nucleotide positions +2 to +34 of vite
Figure 5. (A) Effect of the addition of dephosphorylated MDBP-2-H1 to the in vitro transcription system

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