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Effect of phenanthrene exposure on apoplastic barriers
formation in maize
Joan Dupuy, Pierre Leglize, Quentin Vincent, Ivan Zelko, Christian Mustin,
Stéphanie Ouvrard, Thibault Sterckeman
To cite this version:
Joan Dupuy, Pierre Leglize, Quentin Vincent, Ivan Zelko, Christian Mustin, et al.. Effect of phenan-threne exposure on apoplastic barriers formation in maize. Rhizosphere 4, Jun 2015, Maastricht, Netherlands. 2015. �hal-01264203�
Session : Rhizo Remediation and Fate of Pollutants
Effect of phenanthrene exposure on apoplastic barriers formation in maize Joan Dupuy1,2, Pierre Leglize1,2, Quentin Vincent1,2, Ivan Zelko1,2, Christian Mustin3,4,
Stéphanie Ouvrard1,2, and Thibault Sterckeman1,2 1 Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France 2INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France 3Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, F-54500, France 4CNRS, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, F-54500, France Presenting author email: pierre.leglize@univ-lorraine.fr ABSTRACT
Management of Polycyclic Aromatic Hydrocarbons (PAH) contaminated soils through phytoremediation process is promising in controlled conditions. However, in situ experiments were not so successful due to plant growth limitation induced by PAH toxicity. Improving revegetation of contaminated site appears therefore crucial in phytoremediation application but requires a better understanding of contaminant’s impact on plant functioning. PAH exposure affects plant physiology such as mineral nutrition. Because the nutrient uptake is linked with the maturation of exodermis and endodermis , we focused on (i) the effect of PAH on the suberin lamellae formation and (ii) the localization of phenanthrene in root. Maize plants were grown in contaminated sand (50 and 150 mg PHE kg-1 dry sand) for 10 or 20 days. Epi-fluorescence microscopic
observation of root sections was used to assess PHE localization within the root and maturation of exodermis and endodermis by Fluorol yellow 088 detection of suberin. For 10 days of cultivation, suberization of exodermis and endodermis of maize exposed to PHE was more extensive and PHE were only observed within suberized exodermis. This could be related to an inducer effect of PHE on the deposition of hydrophobic compounds within exodermis, which may act as barrier against PHE penetration. However after 20 days of exposure, exodermis and endodermis of non-exposed roots were totally suberized whereas PHE-exposed roots where less suberized. After 20 days, PHE seemed to inhibit root maturation, which could be caused by its toxicity as it is related with the plant biomass reduction. Furthermore, PHE patches were located only within suberized exodermis and endodermis, which may therefore act as retention zone where the hydrophobic PHE accumulates during its radial transport.
