https://lib.uliege.be https://matheo.uliege.be
Characterization of the virome of ancient pome fruit cultivars of Malus Mill. and Pyrus L. using high-throughput sequencing
Auteur : Fontdevila Pareta, Núria Promoteur(s) : Massart, Sébastien
Faculté : Gembloux Agro-Bio Tech (GxABT)
Diplôme : Master en bioingénieur : sciences agronomiques, à finalité spécialisée Année académique : 2019-2020
URI/URL : http://hdl.handle.net/2268.2/9475
Avertissement à l'attention des usagers :
Tous les documents placés en accès ouvert sur le site le site MatheO sont protégés par le droit d'auteur. Conformément aux principes énoncés par la "Budapest Open Access Initiative"(BOAI, 2002), l'utilisateur du site peut lire, télécharger, copier, transmettre, imprimer, chercher ou faire un lien vers le texte intégral de ces documents, les disséquer pour les indexer, s'en servir de données pour un logiciel, ou s'en servir à toute autre fin légale (ou prévue par la réglementation relative au droit d'auteur). Toute utilisation du document à des fins commerciales est strictement interdite.
Par ailleurs, l'utilisateur s'engage à respecter les droits moraux de l'auteur, principalement le droit à l'intégrité de l'oeuvre et le droit de paternité et ce dans toute utilisation que l'utilisateur entreprend. Ainsi, à titre d'exemple, lorsqu'il reproduira un document par extrait ou dans son intégralité, l'utilisateur citera de manière complète les sources telles que
mentionnées ci-dessus. Toute utilisation non explicitement autorisée ci-avant (telle que par exemple, la modification du
document ou son résumé) nécessite l'autorisation préalable et expresse des auteurs ou de leurs ayants droit.
ANNEX I: D ISTANCE M ATRIXES
Table 1. Distance matrix of RdRp region at nucleotide level from complete sequences of apple stem pitting virus (ASPV) and apple green crinkle associated virus (AGCaV), downloaded from NCBI (https://www.ncbi.nlm.nih.gov/), and complete RdRp sequences from the three new ASPV isolates recovered from tree Q9.
KF32 1967
MK92 3755
MK92 3753
KY49 0039
MK23 9268
AB04 5371
KF31 9056
I3- Q9
MK92 3754
MK83 6301
MK92 3756
JF94 6772
JF94 6775
KJ52 2472
KF91 5809
I2- Q9
KY24 2757
AGCaV_K T835289
AGCaV_NC _018714
FR69 4186
LM99 9967
KF32 1966
KY70 2580
EU09 5327
I1- Q9
KY79 8310
LC47 5151
KY70 2581
D21 829
KU30 8398
LC47 5150
MG76 3895 KF321967 80,807 73,817
73,98 8 73,805
74,50 5
74,31 7
74,
049 73,324 74,027 74,331 74,25
0 74,34
1 74,20
8 76,09
7 74, 307
74,29
9 70,425 73,659
73,15 8 73,318
73,75 1
74,42 6
66,63 6
74, 101
73,56 8
73,81 2
74,77 1
73,4 13
73,88 8
76,26 4 74,311 MK923755
80,80
7 73,589
74,26 2 74,094
74,53 6
74,52 9
74,
490 74,495 74,270 74,164 73,13
8 73,39
7 74,04
0 76,03
6 74, 086
73,76
6 70,937 73,796
73,66 0 73,288
73,96 4
74,06 1
66,49 9
74, 452
74,17 7
73,46 1
74,14 7
73,5 65
73,69 0
76,73 7 74,174 MK923753
73,81 7 73,589
72,88 0 72,788
73,41 3
73,26 6
73,
572 73,367 73,309 74,025 75,70
1 76,64
5 72,74
8 73,08
3 73, 693
72,97
7 70,411 73,403
72,46 0 72,971
73,55 6
73,20 5
66,78 9
73, 541
72,70 2
72,51 9
72,82 4
72,9 13
72,65 7
74,62 3 72,974 KY490039
73,98
8 74,262 72,880 99,756 79,50
6 78,60
5 80,
230 80,299 75,659 77,615 73,95
2 74,12
0 78,66
7 75,60
2 75, 160
75,54
1 72,568 75,816
75,45 0 75,061
75,40 4
75,57 2
67,73 4
75, 816
75,70 9
75,63 3
75,84 6
75,2 17
75,63 3
77,26 4 75,217 MK239268
73,80
5 74,094 72,788 99,75
6
79,32 3
78,43 8
80,
093 80,146 75,553 77,432 73,81
5 73,99
8 78,50
0 75,41
9 74, 977
75,35
8 72,408 75,648
75,26 7 74,878
75,20 6
75,41 9
67,58 1
75, 633
75,54 1
75,43 5
75,66 3
75,0 34
75,46 5
77,03 6 75,050 AB045371
74,50
5 74,536 73,413 79,50
6 79,323
86,75 2
81,
305 81,199 75,095 75,339 74,83
6 75,09
5 77,66
1 75,45
0 75, 099
75,13
0 72,784 75,999
74,87 0 74,741
75,83 1
75,55 7
67,85 6
75, 602
74,64 2
74,26 0
75,37 4
75,4 76
76,34 9
78,01 2 75,995 KF319056
74,31
7 74,529 73,266 78,60
5 78,438 86,75
2
78,
948 79,015 75,969 75,665 74,36
8 74,29
2 76,18
4 75,97
1 76, 321
77,06
7 74,367 77,646
76,54 8 75,658
76,68 6
76,71 8
69,08 7
78, 103
78,17 9
77,44 8
79,47 3
77,6 41
76,38 2
79,39 7 76,606 Isolate 3 Q9
74,04
9 74,490 73,572 80,23
0 80,093 81,30
5 78,94
8 85,598 74,958 75,415 74,90
5 74,91
2 76,48
7 75,29
7 76, 121
75,26
7 73,315 76,388
75,44 2 75,411
75,93 0
75,70 9
67,47 4
75, 862
75,01 5
74,73 3
75,85 4
74,8 06
76,41 0
78,14 1 75,934 MK923754
73,32
4 74,495 73,367 80,29
9 80,146 81,19
9 79,01
5 85,
598 75,312 74,901
74,85 1
75,24 8
76,44 1
75,58 7
75, 450
75,23
6 73,013 76,273
74,50 5 74,497
75,38 9
75,65 1
67,09 3
75, 648
75,32 8
75,09 9
75,84 6
74,6 84
76,50 2
77,70 7 76,376 MK836301
74,02
7 74,270 73,309 75,65
9 75,553 75,09
5 75,96
9 74,
958 75,312 84,150
75,17 5
75,28 2
77,02 5
76,59 8
76, 354
76,84
2 72,761 75,698
76,17 0 75,248
76,70 5
76,49 3
69,03 0
77, 285
77,37 6
76,85 7
77,43 7
76,9 14
75,94 2
78,73 4 76,197 MK923756
74,33
1 74,164 74,025 77,61
5 77,432 75,33
9 75,66
5 75,
415 74,901 84,150
74,65 7
74,85 5
79,37 5
76,67 4
76, 636
76,41
5 73,026 76,003
76,64 3 75,111
76,27 8
76,32 5
69,41 1
77, 742
77,13 2
76,96 4
77,84 9
77,1 27
76,01 8
78,53 5 75,846 JF946772
74,25
0 73,138 75,701 73,95
2 73,815 74,83
6 74,36
8 74,
905 74,851 75,175 74,657
94,03 5
76,33 9
76,70 5
76, 255
76,15
6 73,313 76,552
76,01 8 75,903
77,04 0
77,34 6
70,64 7
76, 812
76,33 9
75,89 6
76,73 5
78,6 52
78,52 0
80,74 8 80,122 JF946775
74,34
1 73,397 76,645 74,12
0 73,998 75,09
5 74,29
2 74,
912 75,248 75,282 74,855 94,03
5
76,23 2
76,78 1
76, 262
76,04
9 73,397 76,659
76,15 5 75,720
76,87 3
77,20 8
71,51 6
77, 132
76,26 2
76,00 3
76,81 2
79,0 03
78,07 8
80,93 1 79,710 KJ522472
74,20
8 74,040 72,748 78,66
7 78,500 77,66
1 76,18
4 76,
487 76,441 77,025 79,375 76,33
9 76,23
2
78,51 0
78, 358
79,30
4 75,402 78,358
78,67 6 78,027
78,90 7
78,87 7
70,64 8
80, 708
79,68 6
79,16 7
79,71 6
79,8 93
78,11 4
80,99 8 78,047 KF915809
76,09
7 76,036 73,083 75,60
2 75,419 75,45
0 75,97
1 75,
297 75,587 76,598 76,674 76,70
5 76,78
1 78,51
0
78, 289
79,01
4 75,295 78,663
79,88 1 78,057
78,66 3
78,51 0
70,58 7
79, 563
79,47 2
78,69 4
80,93 7
79,2 98
78,29 7
81,09 0 78,108 Isolate 2 Q9
74,30
7 74,086 73,693 75,16
0 74,977 75,09
9 76,32
1 76,
121 75,450 76,354 76,636 76,25
5 76,26
2 78,35
8 78,28
9
82,45
6 76,165 79,556
78,84 4 79,216
79,82 3
79,68 6
70,26 7
79, 212
78,22 0
78,06 8
79,65 5
78,7 19
79,35 7
82,07 4 80,267 KY242757
74,29
9 73,766 72,977 75,54
1 75,358 75,13
0 77,06
7 75,
267 75,236 76,842 76,415 76,15
6 76,04
9 79,30
4 79,01
4 82,
456 75,745 79,075
79,53 0 79,658
79,33 5
80,29 6
70,98 4
80, 891
80,63 2
80,81 5
81,82 2
79,8 32
77,83 9
81,02 9 78,795 AGCaV_KT
835289 70,42
5 70,937 70,411 72,56
8 72,408 72,78
4 74,36
7 73,
315 73,013 72,761 73,026 73,31
3 73,39
7 75,40
2 75,29
5 76, 165
75,74
5 92,791
76,86 9 79,824
80,38 0
79,85 3
66,94 9
75, 908
74,67 7
75,24 9
78,84 4
75,2 76
76,27 7
79,05 0 76,786 AGCaV_NC
_018714 73,65
9 73,796 73,403 75,81
6 75,648 75,99
9 77,64
6 76,
388 76,273 75,698 76,003 76,55
2 76,65
9 78,35
8 78,66
3 79, 556
79,07
5 92,791
80,26 2 83,318
83,89 8
82,89 1
69,84 0
79, 289
77,96 1
78,55 6
82,35 7
78,6 12
80,31 1
82,03 6 79,314 FR694186
73,15
8 73,660 72,460 75,45
0 75,267 74,87
0 76,54
8 75,
442 74,505 76,170 76,643 76,01
8 76,15
5 78,67
6 79,88
1 78, 844
79,53
0 76,869 80,262 83,511
81,69 6
81,20 8
71,20 0
79, 515
79,25 6
78,72 2
81,31 5
81,1 56
78,43 2
81,66 6 78,442 LM999967
73,31
8 73,288 72,971 75,06
1 74,878 74,74
1 75,65
8 75,
411 74,497 75,248 75,111 75,90
3 75,72
0 78,02
7 78,05
7 79, 216
79,65
8 79,824 83,318
83,51 1
83,74 5
85,16 3
69,47 1
78, 866
77,67 6
77,76 8
80,74 1
78,6 47
78,54 5
81,42 7 78,936 KF321966
73,75
1 73,964 73,556 75,40
4 75,206 75,83
1 76,68
6 75,
930 75,389 76,705 76,278 77,04
0 76,87
3 78,90
7 78,66
3 79, 823
79,33
5 80,380 83,898
81,69 6 83,745
87,08 8
70,61 8
78, 953
78,61 7
79,38 0
81,36 4
79,1 15
79,19 7
82,32 6 79,924 KY702580
74,42
6 74,061 73,205 75,57
2 75,419 75,55
7 76,71
8 75,
709 75,651 76,493 76,325 77,34
6 77,20
8 78,87
7 78,51
0 79, 686
80,29
6 79,853 82,891
81,20 8 85,163
87,08 8
70,14 5
79, 350
78,95 3
78,73 9
81,34 9
79,0 69
79,12 1
82,37 2 80,702 EU095327
66,63
6 66,499 66,789 67,73
4 67,581 67,85
6 69,08
7 67,
474 67,093 69,030 69,411 70,64
7 71,51
6 70,64
8 70,58
7 70, 267
70,98
4 66,949 69,840
71,20 0 69,471
70,61 8
70,14 5
72, 143
71,28 9
71,71 6
72,25 0
82,3 26
70,28 2
73,80 6 69,915 Isolate 1 Q9
74,10
1 74,452 73,541 75,81
6 75,633 75,60
2 78,10
3 75,
862 75,648 77,285 77,742 76,81
2 77,13
2 80,70
8 79,56
3 79, 212
80,89
1 75,908 79,289
79,51 5 78,866
78,95 3
79,35 0
72,14 3
83,94 4
83,34 9
82,96 7
82,2 43
79,62 5
83,22 6 78,963 KY798310
73,56
8 74,177 72,702 75,70
9 75,541 74,64
2 78,17
9 75,
015 75,328 77,376 77,132 76,33
9 76,26
2 79,68
6 79,47
2 78, 220
80,63
2 74,677 77,961
79,25 6 77,676
78,61 7
78,95 3
71,28 9
83, 944
87,28 6
81,53 2
81,9 22
77,97 6
83,07 4 77,818 LC475151
73,81
2 73,461 72,519 75,63
3 75,435 74,26
0 77,44
8 74,
733 75,099 76,857 76,964 75,89
6 76,00
3 79,16
7 78,69
4 78, 068
80,81
5 75,249 78,556
78,72 2 77,768
79,38 0
78,73 9
71,71 6
83, 349
87,28 6
81,53 2
81,7 39
77,76 3
82,47 9 77,925 KY702581
74,77
1 74,147 72,824 75,84
6 75,663 75,37
4 79,47
3 75,
854 75,846 77,437 77,849 76,73
5 76,81
2 79,71
6 80,93
7 79, 655
81,82
2 78,844 82,357
81,31 5 80,741
81,36 4
81,34 9
72,25 0
82, 967
81,53 2
81,53 2
80,9 92
79,62 5
83,25 7 79,359 D21829
73,41
3 73,565 72,913 75,21
7 75,034 75,47
6 77,64
1 74,
806 74,684 76,914 77,127 78,65
2 79,00
3 79,89
3 79,29
8 78, 719
79,83
2 75,276 78,612
81,15 6 78,647
79,11 5
79,06 9
82,32 6
82, 243
81,92 2
81,73 9
80,99 2
78,78 0
83,95 1 78,728 KU308398
73,88
8 73,690 72,657 75,63
3 75,465 76,34
9 76,38
2 76,
410 76,502 75,942 76,018 78,52
0 78,07
8 78,11
4 78,29
7 79, 357
77,83
9 76,277 80,311
78,43 2 78,545
79,19 7
79,12 1
70,28 2
79, 625
77,97 6
77,76 3
79,62 5
78,7 80
87,85 1 85,919 LC475150
76,26
4 76,737 74,623 77,26
4 77,036 78,01
2 79,39
7 78,
141 77,707 78,734 78,535 80,74
8 80,93
1 80,99
8 81,09
0 82, 074
81,02
9 79,050 82,036
81,66 6 81,427
82,32 6
82,37 2
73,80 6
83, 226
83,07 4
82,47 9
83,25 7
83,9 51
87,85
1 89,184
Table 2. Distance matrix of RdRp region at aminoacid level from complete sequences of apple stem pitting virus (ASPV) and apple green crinkle associated virus (AGCaV), downloaded from NCBI (https://www.ncbi.nlm.nih.gov/), and complete RdRp sequences from the three new ASPV isolates recovered from tree Q9.
EU09 5327
AGCaV_A LV82119
MK92 3753
KF32 1967
MK92 3755
KY49 0039
MK23 9268
AB04 5371
KF31 9056
MK92 3754
I3- Q9
MK83 6301
MK92 3756
FR69 4186
JF94 6772
JF94 6775
AGCaV_B BJ56464
D21 829
KF91 5809
KJ52 2472
KU30 8398
LC47 5150
MG76 3895
KY24 2757
I2- Q9
I1- Q9
KY70 2581
LM99 9967
KF32 1966
KY70 2580
LC47 5151
KY79 8310
EU095327 62.383 65.812
67.07
9 66.850 67.170 66.941 68.11
5 68.70
7 67.566 67.
843 69.093 69.386 68.78
7 69.24
5 69.74
8 68.084
74.8 05
70.14 7
70.55 9 69.872
71.10
8 70.343 71.612 71.
127 71.
612 71.062 69.167 70.28
4 70.330 70.37
5 70.650 AGCaV_A
LV82119 62.38
3 74.746
75.83
4 76.475 78.033 77.804 78.78
5 79.18
0 78.968 78.
970 78.903 78.746 79.60
0 78.08
9 78.22
6 81.498
79.8 74
80.46 1
80.96 4 81.010
82.98
0 81.339 81.148 81.
864 81.
148 83.163 82.055 83.85
0 83.850 79.86
5 80.094 MK923753
65.81
2 74.746
85.53
1 85.440 84.608 84.333 85.35
5 84.87
2 85.217 85.
586 84.936 85.151 81.51
0 86.04
1 86.27
0 81.970
82.8 38
83.97 4
83.92 9 83.974
85.07
3 84.851 84.524 85.
105 84.
844 84.295 82.891 84.75
3 84.570 83.42
5 83.700 KF321967
67.07
9 75.834 85.531 92.736 85.937 85.662
86.36 2
85.98 8 86.426
86.
364 85.956 86.401 83.79
1 84.43
2 84.47
8 84.222
85.3 48
87.12 8
85.79 9 85.479
86.85
3 85.943 85.479 86.
566 86.
441 86.257 84.211 86.25
7 86.185 84.97
5 85.937 MK923755
66.85
0 76.475 85.440 92.73
6 86.532 86.257
86.54 5
86.12 5 86.700
86.
867 86.048 85.806 83.56
2 84.57
0 84.66
1 84.437
84.8 44
87.40 3
86.21 2 85.616
87.03
6 86.218 86.028 86.
657 86.
441 86.532 84.073 86.57
8 86.414 85.29
5 85.937 KY490039
67.17
0 78.033 84.608 85.93
7 86.532 99.725
91.80 0
90.43 9 92.396
92.
077 88.365 88.273 85.94
3 85.66
8 85.62
3 87.090
86.7 67
88.36 5
89.51 0 88.365
89.73
9 88.553 88.639 88.
594 88.
960 88.777 86.362 88.45
6 88.181 86.94
5 87.723 MK239268
66.94
1 77.804 84.333 85.66
2 86.257 99.725
91.52 5
90.16 5 92.121
91.
802 88.090 87.998 85.66
8 85.39
4 85.34
8 86.875
86.4 93
88.09 0
89.23 5 88.090
89.46
4 88.278 88.365 88.
319 88.
685 88.502 86.087 88.18
1 87.907 86.67
0 87.448 AB045371
68.11
5 78.785 85.355 86.36
2 86.545 91.800 91.525
95.38 4 93.547
93.
000 88.924 88.777 85.95
6 85.91
0 86.23
1 87.789
87.3 74
88.83 3
89.29 1 88.879
89.84
0 88.609 87.872 88.
823 88.
924 88.696 86.877 89.19
9 89.062 87.50
6 87.689 KF319056
68.70
7 79.180 84.872 85.98
8 86.125 90.439 90.165 95.38
4 91.648
91.
593 88.498 88.808 86.79
8 85.15
3 85.42
7 87.594
88.3 51
88.75 7
88.89 4 88.894
90.53
9 89.402 89.260 89.
661 89.
854 89.808 87.443 89.44
2 89.457 88.11
7 88.574 MK923754
67.56
6 78.968 85.217 86.42
6 86.700 92.396 92.121 93.54
7 91.64
8
95.
105 88.346 88.243 86.36
8 86.23
1 86.27
6 87.144
86.8 71
88.42 1
88.83 3 88.879
89.97
7 88.975 87.872 88.
823 88.
787 88.787 86.831 89.24
5 88.985 87.41
4 87.689 Isolate 3 Q9
67.84
3 78.970 85.586 86.36
4 86.867 92.077 91.802 93.00
0 91.59
3 95.105 88.423 88.642 86.55
3 86.32
4 86.37
0 87.415
87.1 93
88.37 7
89.15 5 88.927
90.07
1 88.749 88.286 89.
512 88.
835 88.881 87.336 89.38
4 88.927 87.50
8 88.149 MK836301
69.09
3 78.903 84.936 85.95
6 86.048 88.365 88.090 88.92
4 88.49
8 88.346 88.
423 95.050
86.63 0
86.40 1
86.40
1 87.237
88.5 53
88.86 9
89.69 3 88.456
90.42
6 88.645 89.510 89.
821 90.
105 90.060 87.551 89.55
6 89.799 88.59
4 89.372 MK923756
69.38
6 78.746 85.151 86.40
1 85.806 88.273 87.998 88.77
7 88.80
8 88.243 88.
642 95.050
87.07 6
86.29 7
86.25
1 87.130
88.5 88
88.67 5
90.14 2 88.354
90.14
2 88.955 89.821 89.
684 90.
646 90.050 87.540 89.36
3 89.423 88.58
3 89.638 FR694186
68.78
7 79.600 81.510 83.79
1 83.562 85.943 85.668 85.95
6 86.79
8 86.368 86.
553 86.630 87.076
85.12 6
85.30
9 90.689
89.0 62
88.73 6
89.46 9 89.286
91.07
1 89.336 89.469 90.
513 90.
385 90.934 90.348 91.57
5 91.163 88.14
1 89.057 JF946772
69.24
5 78.089 86.041 84.43
2 84.570 85.668 85.394 85.91
0 85.15
3 86.231 86.
324 86.401 86.297 85.12
6
97.61
9 86.598
87.7 80
87.36 3
88.23 3 89.057
90.79
7 91.758 88.233 89.
047 87.
912 88.095 85.910 88.64
5 88.645 86.72
2 87.225 JF946775
69.74
8 78.226 86.270 84.47
8 84.661 85.623 85.348 86.23
1 85.42
7 86.276 86.
370 86.401 86.251 85.30
9 97.61
9 86.652
88.3 75
87.59 2
88.09 5 89.057
91.02
6 91.621 88.370 89.
047 88.
095 88.278 86.185 88.78
2 88.828 86.72
2 87.454 AGCaV_
NC_018714 68.08
4 81.498 81.970 84.22
2 84.437 87.090 86.875 87.78
9 87.59
4 87.144 87.
415 87.237 87.130 90.68
9 86.59
8 86.65
2
88.0 52
89.60 7
90.36 1 90.468
92.40
7 90.258 90.361 91.
007 90.
199 92.784 91.438 92.73
0 92.623 88.96
1 89.715 D21829
74.80
5 79.874 82.838 85.34
8 84.844 86.767 86.493 87.37
4 88.35
1 86.871 87.
193 88.553 88.588 89.06
2 87.78
0 88.37
5 88.052
90.70 5
91.16 3 90.293
92.58
2 90.847 91.758 91.
751 92.
720 91.896 88.838 90.38
5 91.071 91.52
9 91.667 KF915809
70.14
7 80.461 83.974 87.12
8 87.403 88.365 88.090 88.83
3 88.75
7 88.421 88.
377 88.869 88.675 88.73
6 87.36
3 87.59
2 89.607
90.7 05
90.74 7 90.380
92.44
2 90.888 91.800 91.
701 92.
808 92.304 89.474 91.02
2 91.205 90.74
7 91.113 KJ522472
70.55
9 80.964 83.929 85.79
9 86.212 89.510 89.235 89.29
1 88.89
4 88.833 89.
155 89.693 90.142 89.46
9 88.23
3 88.09
5 90.361
91.1 63
90.74
7 91.113
92.99
1 91.438 92.533 91.
976 92.
716 92.900 90.252 92.12
1 92.258 90.70
1 91.022 KU308398
69.87
2 81.010 83.974 85.47
9 85.616 88.365 88.090 88.87
9 88.89
4 88.879 88.
927 88.456 88.354 89.28
6 89.05
7 89.05
7 90.468
90.2 93
90.38 0
91.11 3
95.09
8 94.414 91.434 92.
618 91.
709 91.663 89.657 91.89
2 91.571 90.38
0 90.518 LC475150
71.10
8 82.980 85.073 86.85
3 87.036 89.739 89.464 89.84
0 90.53
9 89.977 90.
071 90.426 90.142 91.07
1 90.79
7 91.02
6 92.407
92.5 82
92.44 2
92.99
1 95.098 95.971 93.816 94.
865 93.
953 94.182 92.128 94.27
4 94.320 92.62
5 93.083 MG763895
70.34
3 81.339 84.851 85.94
3 86.218 88.553 88.278 88.60
9 89.40
2 88.975 88.
749 88.645 88.955 89.33
6 91.75
8 91.62
1 90.258
90.8 47
90.88 8
91.43 8 94.414
95.97
1 91.941
93.
309 91.
987 92.308 90.256 92.49
1 92.674 90.29
3 90.888 KY242757
71.61
2 81.148 84.524 85.47
9 86.028 88.639 88.365 87.87
2 89.26
0 87.872 88.
286 89.510 89.821 89.46
9 88.23
3 88.37
0 90.361
91.7 58
91.80 0
92.53 3 91.434
93.81 6 91.941
95.
002 93.
678 93.953 90.389 92.35
0 92.533 92.12
1 92.900 Isolate 2 Q9
71.12
7 81.864 85.105 86.56
6 86.657 88.594 88.319 88.82
3 89.66
1 88.823 89.
512 89.821 89.684 90.51
3 89.04
7 89.04
7 91.007
91.7 51
91.70 1
91.97 6 92.618
94.86
5 93.309 95.002 93.
122 93.122 91.388 93.16
8 93.306 91.42
6 91.884 Isolate 1 Q9
71.61
2 81.148 84.844 86.44
1 86.441 88.960 88.685 88.92
4 89.85
4 88.787 88.
835 90.105 90.646 90.38
5 87.91
2 88.09
5 90.199
92.7 20
92.80 8
92.71 6 91.709
93.95
3 91.987 93.678 93.
122 94.411 90.755 92.44
2 92.945 93.12
9 93.770 KY702581
71.06
2 83.163 84.295 86.25
7 86.532 88.777 88.502 88.69
6 89.80
8 88.787 88.
881 90.060 90.050 90.93
4 88.09
5 88.27
8 92.784
91.8 96
92.30 4
92.90 0 91.663
94.18
2 92.308 93.953 93.
122 94.
411 92.449
94.41 1 94.503
91.98 4 92.579 LM999967
69.16
7 82.055 82.891 84.21
1 84.073 86.362 86.087 86.87
7 87.44
3 86.831 87.
336 87.551 87.540 90.34
8 85.91
0 86.18
5 91.438
88.8 38
89.47 4
90.25 2 89.657
92.12
8 90.256 90.389 91.
388 90.
755 92.449
93.04 3 93.867
88.83 3 89.291 KF321966
70.28
4 83.850 84.753 86.25
7 86.578 88.456 88.181 89.19
9 89.44
2 89.245 89.
384 89.556 89.363 91.57
5 88.64
5 88.78
2 92.730
90.3 85
91.02 2
92.12 1 91.892
94.27
4 92.491 92.350 93.
168 92.
442 94.411 93.043 96.290 90.56
3 90.884 KY702580
70.33
0 83.850 84.570 86.18
5 86.414 88.181 87.907 89.06
2 89.45
7 88.985 88.
927 89.799 89.423 91.16
3 88.64
5 88.82
8 92.623
91.0 71
91.20 5
92.25 8 91.571
94.32
0 92.674 92.533 93.
306 92.
945 94.503 93.867 96.29
0
90.97 6 91.342 LC475151
70.37
5 79.865 83.425 84.97
5 85.295 86.945 86.670 87.50
6 88.11
7 87.414 87.
508 88.594 88.583 88.14
1 86.72
2 86.72
2 88.961
91.5 29
90.74 7
90.70 1 90.380
92.62
5 90.293 92.121 91.
426 93.
129 91.984 88.833 90.56
3 90.976 93.907
Table 3. Distance matrix of CP region at nucleotide level from complete sequences of apple stem pitting virus (ASPV) and apple green crinkle associated virus (AGCaV), downloaded from NCBI (https://www.ncbi.nlm.nih.gov/), and complete CP sequences from the three new ASPV isolates recovered from tree Q9.
LM99 9967
MK92 3753
KY24 2757
JF94 6775
JF94 6772
MK92 3755
KF32 1967
AGCaV_H E963831
AGCaV_K T835289
MG76 3895
KF31 9056
KJ52 2472
KY70 2580
I2- Q9
KY79 8310
LC47 5151
I1- Q9
FR69 4186
AB04 5371
I3- Q9
MK92 3754
MK92 3756
KF91 5809
MK83 6301
KF32 1966
KY49 0039
MK23 9268
KU30 8398
KY70 2581
LC47 5150
D21 829
EU09 5327
LM999967 75.870 77.250 66.24
0 65.99
0 63.387 64.43
5 62.440 61.089 63.483
65.38 5
63.62 2 64.173
66.
613 63.738 63.01
9 68.
517 65.86
6 64.56
7 65.
668 66.114 64.173 64.17
3 65.433 64.17
3 64.789 64.623 64.677 65.617 67.73
7 64.2
35 59.65
8 MK923753 75.870 88.372
66.34 8
65.69 8 64.026
64.26
8 61.422 60.596 61.981
65.79 8
64.81 2 64.577
65.
885 65.245 64.92
4 67.
106 66.77
7 64.57
7 65.
460 66.117 65.909 64.18
5 64.655 63.55
8 64.056 63.891 64.851 66.447 66.45
4 62.9
04 58.96
2 KY242757 77.250 88.372
67.45 7
65.90 4 65.830
65.18
2 61.840 60.853 63.622
67.63 0
67.71 3 67.217
66.
309 65.109 64.46
4 67.
795 67.05
2 66.96
9 67.
022 67.135 67.382 66.22
6 68.538 66.22
6 64.822 64.657 64.988 68.126 68.70
4 63.7
15 59.28
0 JF946775 66.240 66.348 67.457
90.06 6 65.590
63.24
7 65.425 63.860 65.481
66.88 5
66.55 9 65.997
67.
779 64.000 62.96
0 67.
884 68.63
1 66.80
1 68.
182 67.967 68.489 68.65
0 68.167 67.44
4 68.465 68.299 68.631 69.959 69.29
3 65.1
31 60.00
0 JF946772 65.990 65.698 65.904
90.06
6 64.459
64.21
1 65.830 64.143 65.319
66.88 5
67.70 0 67.292
67.
292 64.400 64.64
0 68.
548 68.63
1 67.53
7 67.
839 68.216 69.005 68.51
5 69.168 66.55
8 67.303 67.137 68.465 70.290 70.14
7 65.6
87 61.06
1 MK923755 63.387 64.026 65.830
65.59 0
64.45 9
79.26
8 63.622 62.634 65.360
67.23 9
66.99 3 66.830
68.
056 66.825 64.84
1 69.
199 68.38
2 68.21
9 68.
017 68.709 68.412 69.06
7 70.434 68.87
3 68.056 67.892 67.484 70.016 71.32
4 66.1
19 61.97
4 KF321967 64.435 64.268 65.182
63.24 7
64.21
1 79.268 66.026 64.476 66.000
68.30 1
67.64 7 67.974
68.
791 66.587 65.07
9 69.
118 69.11
8 67.56
5 67.
806 68.627 67.974 67.97
4 68.301 67.56
5 67.484 67.320 66.912 69.281 71.07
8 65.7
28 61.00
3 AGCaV_
NC_018714 62.440 61.422 61.840 65.42
5 65.83
0 63.622 66.02
6 95.765 75.470
69.35 0
67.96 7 67.561
68.
374 63.608 63.05
9 68.
537 67.80
5 67.88
6 67.
926 70.000 68.293 67.15
4 68.049 69.10
6 69.593 69.431 66.504 69.919 70.32
5 64.0
97 57.89
0 AGCaV_K
T835289 61.089 60.596 60.853 63.86
0 64.14
3 62.634 64.47
6 95.765 73.194
67.20 9
66.32 8 65.962
66.
965 62.810 62.13
1 67.
263 66.39
6 66.31
4 66.
261 68.631 67.087 65.50
1 66.436 67.61
5 67.710 67.547 64.770 68.144 68.46
9 62.5
52 56.41
4 MG763895 63.483 61.981 63.622
65.48 1
65.31 9 65.360
66.00
0 75.470 73.194
66.31 5
67.37 0 66.964
66.
071 62.569 63.43
0 66.
883 67.45
1 66.55
8 66.
178 68.101 66.234 66.55
8 67.695 66.80
2 66.477 66.315 64.367 68.994 73.13
3 63.9
97 58.85
7 KF319056 65.385 65.798 67.630
66.88 5
66.88 5 67.239
68.30
1 69.350 67.209 66.315
77.19 7 76.202
77.
446 72.977 72.24
9 77.
162 77.49
8 77.61
2 78.
472 78.925 74.627 76.03
6 74.627 76.03
6 74.475 74.475 73.971 76.574 77.03
2 71.1
65 66.00
7 KJ522472 63.622 64.812 67.713
66.55 9
67.70 0 66.993
67.64
7 67.967 66.328 67.370
77.19
7 96.772
78.
272 74.595 72.41
1 78.
505 78.17
0 76.63
5 78.
733 79.513 75.120 74.40
2 73.764 73.84
4 75.231 75.063 73.132 74.727 76.42
3 71.3
25 66.00
7 KY702580 64.173 64.577 67.217
65.99 7
67.29 2 66.830
67.97
4 67.561 65.962 66.964
76.20 2
96.77 2
78.
107 74.029 72.33
0 77.
582 77.75
0 76.31
6 78.
819 78.841 74.561 74.16
3 73.764 72.72
7 74.727 74.559 72.544 75.231 76.01
6 71.2
45 65.34
7 Isolate 2 Q9 66.613 65.885 66.309
67.77 9
67.29 2 68.056
68.79
1 68.374 66.965 66.071
77.44 6
78.27
2 78.107 74.676
72.49 2
80.
017 79.76
5 79.67
1 81.
076 80.017 77.942 78.35
4 77.449 77.11
9 79.093 78.925 75.399 78.505 79.83
5 75.0
20 69.47
2 KY798310 63.738 65.245 65.109
64.00 0
64.40 0 66.825
66.58
7 63.608 62.810 62.569
72.97 7
74.59 5 74.029
74.
676
82.28 2
78.
398 78.15
5 73.78
6 74.
812 74.110 71.764 72.97
7 72.654 72.08
7 70.469 70.307 71.278 72.006 75.80
9 69.1
47 64.18
3 LC475151 63.019 64.924 64.464
62.96 0
64.64 0 64.841
65.07
9 63.059 62.131 63.430
72.24 9
72.41 1 72.330
72.
492 82.282
76.
214 76.29
4 72.08
7 72.
891 72.654 70.550 72.08
7 71.521 70.79
3 70.388 70.227 71.197 70.227 73.86
7 67.3
64 62.74
0 Isolate 1 Q9 68.517 67.106 67.795
67.88 4
68.54 8 69.199
69.11
8 68.537 67.263 66.883
77.16 2
78.50 5 77.582
80.
017 78.398 76.21
4
83.96 3
79.59 7
79.
384 80.353 76.742 76.91
0 77.666 77.75
0 77.498 77.330 74.979 76.574 79.09
3 73.6
55 68.66
2 FR694186 65.866 66.777 67.052
68.63 1
68.63 1 68.382
69.11
8 67.805 66.396 67.451
77.49 8
78.17 0 77.750
79.
765 78.155 76.29
4 83.
963
81.78 0
81.
424 80.101 76.406 77.75
0 78.170 77.07
8 77.834 77.750 75.903 78.086 79.93
3 73.0
12 66.83
3 AB045371 64.567 64.577 66.969
66.80 1
67.53 7 68.219
67.56
5 67.886 66.314 66.558
77.61 2
76.63 5 76.316
79.
671 73.786 72.08
7 79.
597 81.78
0
82.
161 83.375 76.156 75.99
7 76.874 75.67
8 77.246 77.162 75.315 79.177 76.58
5 73.0
92 68.06
9 Isolate 3 Q9 65.668 65.460 67.022
68.18 2
67.83 9 68.017
67.80
6 67.926 66.261 66.178
78.47 2
78.73 3 78.819
81.
076 74.812 72.89
1 79.
384 81.42
4 82.16
1 86.285 77.865
77.73 4 78.342
77.47
4 78.819 78.733 74.826 77.170 77.99
5 74.5
66 70.31
5 MK923754 66.114 66.117 67.135
67.96 7
68.21 6 68.709
68.62
7 70.000 68.631 68.101
78.92 5
79.51 3 78.841
80.
017 74.110 72.65
4 80.
353 80.10
1 83.37
5 86.
285 77.750
78.84 1 78.338
79.26
1 79.177 79.009 74.391 79.261 79.00
9 73.7
35 68.32
9 MK923756 64.173 65.909 67.382
68.48 9
69.00 5 68.412
67.97
4 68.293 67.087 66.234
74.62 7
75.12 0 74.561
77.
942 71.764 70.55
0 76.
742 76.40
6 76.15
6 77.
865 77.750
83.61 3 84.307
77.35
2 77.246 77.246 76.490 78.589 78.37
4 74.9
40 69.30
7 KF915809 64.173 64.185 66.226
68.65 0
68.51 5 69.067
67.97
4 67.154 65.501 66.558
76.03 6
74.40 2 74.163
78.
354 72.977 72.08
7 76.
910 77.75
0 75.99
7 77.
734 78.841 83.613 86.890 77.35
2 78.673 78.589 78.002 80.688 79.67
5 76.2
25 69.38
9 MK836301 65.433 64.655 68.538
68.16 7
69.16 8 70.434
68.30
1 68.049 66.436 67.695
74.62 7
73.76 4 73.764
77.
449 72.654 71.52
1 77.
666 78.17
0 76.87
4 78.
342 78.338 84.307 86.89
0
78.46
9 78.086 78.002 78.505 80.772 79.26
8 76.3
05 70.29
7 KF321966 64.173 63.558 66.226
67.44 4
66.55 8 68.873
67.56
5 69.106 67.615 66.802
76.03 6
73.84 4 72.727
77.
119 72.087 70.79
3 77.
750 77.07
8 75.67
8 77.
474 79.261 77.352 77.35
2 78.469 87.741 87.573 80.269 81.528 82.35
8 78.0
72 72.27
7 KY490039 64.789 64.056 64.822
68.46 5
67.30 3 68.056
67.48
4 69.593 67.710 66.477
74.47 5
75.23 1 74.727
79.
093 70.469 70.38
8 77.
498 77.83
4 77.24
6 78.
819 79.177 77.246 78.67
3 78.086 87.74
1 99.832 80.101 81.612 82.28
4 77.5
10 71.07
2 MK239268 64.623 63.891 64.657
68.29 9
67.13 7 67.892
67.32
0 69.431 67.547 66.315
74.47 5
75.06 3 74.559
78.
925 70.307 70.22
7 77.
330 77.75
0 77.16
2 78.
733 79.009 77.246 78.58
9 78.002 87.57
3 99.832 80.185 81.444 82.11
6 77.3
49 70.90
6 KU308398 64.677 64.851 64.988
68.63 1
68.46 5 67.484
66.91
2 66.504 64.770 64.367
73.97 1
73.13 2 72.544
75.
399 71.278 71.19
7 74.
979 75.90
3 75.31
5 74.
826 74.391 76.490 78.00
2 78.505 80.26
9 80.101 80.185 79.849 82.95
5 77.3
49 71.15
5 KY702581 65.617 66.447 68.126
69.95 9
70.29 0 70.016
69.28
1 69.919 68.144 68.994
76.57 4
74.72 7 75.231
78.
505 72.006 70.22
7 76.
574 78.08
6 79.17
7 77.
170 79.261 78.589 80.68
8 80.772 81.52
8 81.612 81.444 79.849
83.79 5
76.8 67
71.48 8 LC475150 67.737 66.454 68.704
69.29 3
70.14 7 71.324
71.07
8 70.325 68.469 73.133
77.03 2
76.42 3 76.016
79.
835 75.809 73.86
7 79.
093 79.93
3 76.58
5 77.
995 79.009 78.374 79.67
5 79.268 82.35
8 82.284 82.116 82.955 83.795
80.9 64
75.00 0 D21829 64.235 62.904 63.715
65.13 1
65.68 7 66.119
65.72
8 64.097 62.552 63.997
71.16 5
71.32 5 71.245
75.
020 69.147 67.36
4 73.
655 73.01
2 73.09
2 74.
566 73.735 74.940 76.22
5 76.305 78.07
2 77.510 77.349 77.349 76.867 80.96
4
89.85 9
Table 4. Distance matrix of CP region at aminoacid level from complete sequences of apple stem pitting virus (ASPV) and apple green crinkle associated virus (AGCaV), downloaded from NCBI (https://www.ncbi.nlm.nih.gov/), and complete CP sequences from the three new ASPV isolates recovered from tree Q9.
EU09 5327
MG76 3895
AGCaV_H E963831
AGCaV_K T835289
MK92 3755
KF32 1967
LC47 5151
KY79 8310
KF31 9056
D21 829
KJ52 2472
KY70 2580
I1- Q9
I2- Q9
FR69 4186
AB04 5371
I3- Q9
MK92 3754
MK92 3756
KF91 5809
MK83 6301
KY70 2581
KF32 1966
KY49 0039
MK23 9268
KU30 8398
LC47 5150
JF94 6775
JF94 6772
LM99 9967
MK92 3753
KY24 2757 EU095327 30.345 30.115 27.701 33.563
32.87 4
38.11 8 37.412
34.50 7
48.7 00
36.15 0 35.211
37.
825 38.
732 37.11
6 38.26
3 37.
317 38.298 37.089 38.49
8 38.028 39.716 38.26
3 38.061 38.298 38.061 39.67
1 30.07
0 30.76
9 30.465 32.558 32.553 MG763895
30.34
5 83.375 77.329 74.510
74.51 0
68.55 8 67.612
72.05 9
68.5 45
73.77 5 73.529
71.
814 72.
549 72.30
4 72.79
4 74.
177 74.020 72.794 73.03
9 73.775 73.529 73.77
5 72.304 72.059 69.118 72.79
4 72.79
4 72.79
4 71.463 69.756 70.488 AGCaV_
NC_018714 30.11
5 83.375 91.473 74.510
75.00 0
68.55 8 69.031
73.77 5
69.2 49
74.51 0 75.000
73.
284 74.
265 74.26
5 74.26
5 74.
684 75.245 74.265 74.26
5 74.755 75.490 73.52
9 74.265 74.020 70.588 72.79
4 72.05
9 72.05
9 70.732 70.488 71.220 AGCaV_K
T835289 27.70
1 77.329 91.473 69.274
69.51 9
63.96 9 64.206
68.77 2
64.6 93
69.26 2 69.753
68.
527 69.
262 69.01
7 69.01
7 69.
758 69.998 68.527 68.77
2 69.262 70.243 68.77
2 69.753 69.508 65.831 68.03
7 67.79
2 67.05
7 66.729 65.998 66.729 MK923755
33.56
3 74.510 74.510 69.274
88.20 6
70.21 3 70.922
75.00 0
71.5 96
77.45 1 76.225
76.
471 76.
961 77.69
6 78.18
6 78.
987 80.147 78.378 78.37
8 78.870 77.941 78.18
6 77.206 76.961 72.794 77.20
6 71.25
3 71.74
4 70.244 71.951 71.951 KF321967
32.87
4 74.510 75.000 69.519 88.206
69.97 6 70.449
74.02 0
70.6 57
75.98 0 74.755
75.
245 76.
225 76.47
1 76.96
1 77.
919 77.696 76.716 76.22
5 77.451 76.471 77.45
1 76.225 75.980 70.588 76.22
5 69.60
8 70.09
8 70.000 70.976 70.976 LC475151
38.11
8 68.558 68.558 63.969 70.213 69.97
6 81.752
74.93 9
77.8 85
77.85 9 76.886
79.
805 78.
589 81.26
5 79.56
2 80.
151 79.075 78.102 78.58
9 78.345 77.859 79.07
5 78.345 78.345 75.669 79.07
5 67.15
0 67.87
4 67.874 68.599 68.357 KY798310
37.41
2 67.612 69.031 64.206 70.922 70.44
9 81.75
2
76.64 2
80.5 29
77.61 6 77.129
82.
238 81.
022 82.23
8 81.50
9 82.
663 82.968 79.319 80.04
9 80.535 80.049 80.29
2 79.319 79.075 78.832 81.02
2 68.11
6 67.87
4 68.599 68.357 68.841 KF319056
34.50
7 72.059 73.775 68.772 75.000 74.02
0 74.93
9 76.642
77.0 53
82.04 5 81.297
82.
828 83.
042 83.08
1 83.79
1 84.
595 86.111 81.546 82.04
5 81.796 85.354 82.79
3 83.333 83.081 79.545 81.54
6 71.04
0 69.80
2 69.136 72.593 73.000 D21829
48.70
0 68.545 69.249 64.693 71.596 70.65
7 77.88
5 80.529 77.05
3
78.01 9 77.053
80.
193 82.
126 80.19
3 80.43
5 80.
798 81.884 80.193 81.40
1 82.126 83.333 81.88
4 81.884 81.643 82.609 85.74
9 69.54
4 70.26
4 69.378 70.574 70.813 KJ522472
36.15
0 73.775 74.510 69.262 77.451 75.98
0 77.85
9 77.616 82.04
5 78.0
19 98.058
83.
333 84.
901 86.11
1 83.73
8 88.
512 87.879 83.738 84.70
9 83.738 85.101 82.03
9 84.091 83.838 79.798 81.90
7 69.41
7 69.45
8 69.231 71.394 73.000 KY702580
35.21
1 73.529 75.000 69.753 76.225 74.75
5 76.88
6 77.129 81.29
7 77.0
53 98.05
8
82.
576 84.
406 85.10
1 82.52
4 87.
467 86.869 82.767 83.73
8 82.767 84.091 80.58
3 83.586 83.333 79.293 81.41
8 69.90
3 69.95
1 68.510 70.192 72.250 Isolate 1 Q9
37.82
5 71.814 73.284 68.527 76.471 75.24
5 79.80
5 82.238 82.82
8 80.1
93 83.33
3 82.576 88.
131 89.64
6 89.39
4 88.
251 88.384 85.859 86.36
4 87.121 86.616 85.35
4 87.121 86.869 81.313 84.59
6 70.17
5 70.42
6 70.000 72.250 72.000 Isolate 2 Q9
38.73
2 72.549 74.265 69.262 76.961 76.22
5 78.58
9 81.022 83.04
2 82.1
26 84.90
1 84.406 88.
131
88.88 9
88.36 6
89.
817 89.141 87.129 86.63
4 87.871 88.636 85.14
9 87.374 87.121 82.828 85.89
1 71.74
4 70.93
6 69.363 71.569 72.750 FR694186
37.11
6 72.304 74.265 69.017 77.696 76.47
1 81.26
5 82.238 83.08
1 80.1
93 86.11
1 85.101 89.
646 88.
889
88.63 6
90.
078 88.384 86.111 87.87
9 87.879 86.869 86.11
1 86.364 86.111 81.818 86.36
4 70.67
7 70.67
7 70.250 71.750 71.500 AB045371
38.26
3 72.794 74.265 69.017 78.186 76.96
1 79.56
2 81.509 83.79
1 80.4
35 83.73
8 82.524 89.
394 88.
366 88.63
6
91.
906 92.424 86.571 85.13
2 85.372 88.384 83.45
3 86.616 86.364 82.323 83.37
4 69.90
3 70.19
7 67.458 71.259 74.000 Isolate 3 Q9
37.31
7 74.177 74.684 69.758 78.987 77.91
9 80.15
1 82.663 84.59
5 80.7
98 88.51
2 87.467 88.
251 89.
817 90.07
8 91.90
6 93.734 89.556
89.81
7 90.339 87.467 86.94
5 86.945 86.684 82.768 85.64
0 73.31
6 72.02
1 72.093 73.127 73.643 MK923754
38.29
8 74.020 75.245 69.998 80.147 77.69
6 79.07
5 82.968 86.11
1 81.8
84 87.87
9 86.869 88.
384 89.
141 88.38
4 92.42
4 93.
734 89.141
89.39
4 89.646 89.141 87.87
9 87.626 87.374 82.323 86.86
9 72.43
1 72.43
1 72.000 73.750 74.500 MK923756
37.08
9 72.794 74.265 68.527 78.378 76.71
6 78.10
2 79.319 81.54
6 80.1
93 83.73
8 82.767 85.
859 87.
129 86.11
1 86.57
1 89.
556 89.141
90.86
5 91.106 87.879 83.45
3 85.606 85.859 82.828 83.61
9 70.56
0 70.61
7 67.458 70.309 72.500 KF915809
38.49
8 73.039 74.265 68.772 78.378 76.22
5 78.58
9 80.049 82.04
5 81.4
01 84.70
9 83.738 86.
364 86.
634 87.87
9 85.13
2 89.
817 89.394 90.865 91.587 89.141 83.21
3 85.859 86.111 82.828 84.10
8 70.31
6 71.11
1 68.409 70.546 72.750 MK836301
38.02
8 73.775 74.755 69.262 78.870 77.45
1 78.34
5 80.535 81.79
6 82.1
26 83.73
8 82.767 87.
121 87.
871 87.87
9 85.37
2 90.
339 89.646 91.106 91.58
7 89.141
84.41
2 86.616 86.869 83.333 84.84
1 71.95
1 72.34
6 69.834 70.309 73.750 KY702581
39.71
6 73.529 75.490 70.243 77.941 76.47
1 77.85
9 80.049 85.35
4 83.3
33 85.10
1 84.091 86.
616 88.
636 86.86
9 88.38
4 87.
467 89.141 87.879 89.14
1 89.141
88.88
9 89.394 89.141 85.606 88.38
4 71.93
0 72.18
0 71.250 74.250 74.250 KF321966
38.26
3 73.775 73.529 68.772 78.186 77.45
1 79.07
5 80.292 82.79
3 81.8
84 82.03
9 80.583 85.
354 85.
149 86.11
1 83.45
3 86.
945 87.879 83.453 83.21
3 84.412 88.889 92.929 92.677 83.586 86.30
8 70.63
1 70.44
3 68.646 70.071 73.250 KY490039
38.06
1 72.304 74.265 69.753 77.206 76.22
5 78.34
5 79.319 83.33
3 81.8
84 84.09
1 83.586 87.
121 87.
374 86.36
4 86.61
6 86.
945 87.626 85.606 85.85
9 86.616 89.394 92.92
9 99.747 84.596
87.37 4
71.42 9
70.67
7 70.750 72.500 72.750 MK239268
38.29
8 72.059 74.020 69.508 76.961 75.98
0 78.34
5 79.075 83.08
1 81.6
43 83.83
8 83.333 86.
869 87.
121 86.11
1 86.36
4 86.
684 87.374 85.859 86.11
1 86.869 89.141 92.67
7 99.747 84.848
87.12 1
71.17 8
70.42
6 70.500 72.250 72.500 KU308398
38.06
1 69.118 70.588 65.831 72.794 70.58
8 75.66
9 78.832 79.54
5 82.6
09 79.79
8 79.293 81.
313 82.
828 81.81
8 82.32
3 82.
768 82.323 82.828 82.82
8 83.333 85.606 83.58
6 84.596 84.848
88.13 1
72.18 0
73.68
4 70.500 72.500 72.000 LC475150
39.67
1 72.794 72.794 68.037 77.206 76.22
5 79.07
5 81.022 81.54
6 85.7
49 81.90
7 81.418 84.
596 85.
891 86.36
4 83.37
4 85.
640 86.869 83.619 84.10
8 84.841 88.384 86.30
8 87.374 87.121 88.131
71.84 5
73.64
5 70.944 72.639 74.250 JF946775
30.07
0 72.794 72.059 67.792 71.253 69.60
8 67.15
0 68.116 71.04
0 69.5
44 69.41
7 69.903 70.
175 71.
744 70.67
7 69.90
3 73.
316 72.431 70.560 70.31
6 71.951 71.930 70.63
1 71.429 71.178 72.180 71.84
5
91.60
5 73.913 73.188 76.559 JF946772
30.76
9 72.794 72.059 67.057 71.744 70.09
8 67.87
4 67.874 69.80
2 70.2
64 69.45
8 69.951 70.
426 70.
936 70.67
7 70.19
7 72.
021 72.431 70.617 71.11
1 72.346 72.180 70.44
3 70.677 70.426 73.684 73.64
5 91.60
5 74.510 73.039 75.810 LM999967
30.46
5 71.463 70.732 66.729 70.244 70.00
0 67.87
4 68.599 69.13
6 69.3
78 69.23
1 68.510 70.
000 69.
363 70.25
0 67.45
8 72.
093 72.000 67.458 68.40
9 69.834 71.250 68.64
6 70.750 70.500 70.500 70.94
4 73.91
3 74.51
0 79.529 85.213
MK923753 32.55
8 69.756 70.488 65.998 71.951 70.97
6 68.59
9 68.357 72.59
3 70.5
74 71.39
4 70.192 72.
250 71.
569 71.75
0 71.25
9 73.
127 73.750 70.309 70.54
6 70.309 74.250 70.07
1 72.500 72.250 72.500 72.63
9 73.18
8 73.03
9 79.529 93.216
