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High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein--DNA complexes

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Academic year: 2021

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Figure 1. AFM images of M13 ssDNA–E. coli SSB on mica with a concentration ratio R [¼(SSB tetramers)/(nucleotides)] equal to 1/30 in (a) Tris 20 mM pH 7.5, NaCl 20 mM, MgCl 2 10 mM and, (b) Tris 20 mM pH 7.5, NaCl 20 mM, SpdCl 3 50 mM (scale bars 500 nm).
Figure 2. (a) Agarose gel electrophoresis of M13 ssDNA–E. coli SSB complexes formed in spermidine buffer Tris 20 mM pH 7.5, NaCl 20 mM, SpdCl 3 50 mM with increasing E
Figure 3. (a) Agarose gel electrophoresis of M13 ssDNA–E. coli SSB complexes formed in spermidine buffer Tris 20 mM pH 7.5, NaCl 300 mM, SpdCl 3 300 mM with increasing E

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