Detection and serotyping of plum pox virus (PPV) isolates by monoclonal antibodies Myrta A., Boscia D., Gavriel I. in

Myrta A., Boscia D., Gavriel I.

in

Di Terlizzi B. (ed.), Myrta A. (ed.), Savino V. (ed.).

Stone fruit viruses and certification in the Mediterranean countries: problems and prospects

Bari : CIHEAM

Options Méditerranéennes : Série B. Etudes et Recherches; n. 19 1998

pages 211-215

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--- Myrta A., Boscia D ., Gavriel I. Detection an d serotypin g of plu m pox viru s ( PPV) isolates by mon oclon al an tibodies. In : D i Terlizzi B. (ed.), Myrta A. (ed.), Savino V. (ed.). Stone fruit viruses and certification in the Mediterranean countries: problems and prospects. Bari : CIHEAM, 1998. p. 211-215 (Options Méditerranéennes : Série B. Etudes et Recherches; n. 19)

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Arben

of and Food

di Studio del sui e le delle

of

Section Nicosia

of Lija

Christina

Athens

Biagio

Valenzano,

Antakya,

Gama1 A.

Giza (Egypt)

Faculty of

Vito

di delle dalle

of

-

antibodies was out in

and No was found in Lebanon and

antigenic analyses among 170 isolates peach and plum

six that they belong to isolates

(139), (20), EA (7) and (4) a of -F was detected in Albania,

Options Méditerranéennes, Série B / n ^O 19, - Stone fruit viruses and certification in the Mediterranean: problems and prospects

212 A. et nl.

and in Albania and in two

in two Egyptian localities

only by the speci€ic to El is the of a

of isolates in

word: stone antibodies,

-

L’enquête n été conduite en Albanie, à Grèce, en nu Liban, B et en Turquie. Au Liban et ¿ì on n’a pas trouvé de ce qui conjïrme les données précédemment mpportées. L’nnnlyse de 170 isolats de B pnrtir de matériel d‘nbricotier, pêcher et prunier, provenant de six pays méditerranéens, a montré qu‘ils appartiennent nux isolnts (139), D (20), (7) et aux mélanges naturels de souches (4), respectivement. La souche d u a été nzise e n évidence en Albanie, li Chypre, en Grèce, ; la souche D a été signalée en Albanie et que des échantillons contenant des mélanges naturels des deux souches ont été repérés dans quelques vergers d’abricotier infectés par le originaires de deux locnlités égyptiennes, n’ont été reconnus que par l‘anticorps monoclonnl spéccifique pour le sérotype Amar.

C‘est ln première fois que, sur une grande échelle, on regroupe en sérotypes des isolats du provenant de plusieurs pays méditerranéens,

essences B noynux, souche de diagnostic sérologique,

pox causing heavy losses in stone was detected in

its was and

Smith, 1994).

The isolates fall in and

as and

analyses of the (Wetzel

1991;

assay based on

al.,

1995; Nemchinov and 1997), and in of

dissociated coat

and used to

type to suitable both the

of and antibodies

with this

al., e t al., et aZ.,

1998) and

1998).

Detection and seroiging of pltrm pox virus isolates by monoclonal antibodies 213

of PPV foci

detailed on was

and done and using

to detect all

Cyprus. One of 672 samples stone (peach, almond,

and plum), was 16% in plum, 12,6% in

and 9,8% in No ne of the 249 almond and 224 samples tested was positive

Field inspections out in and home

tests in and plum the samples tested

negative data an et aZ.,1996).

This study was on and in a

also out in All tested samples and

the status of the islands

(Gatt et al., 1998).

Turkey. None of the selected: 226 plum, 218 215 201 peach, and 65

sampled East and was positive

2. Serotyping of PPV isolates

A study was conducted to isolates with monoclonal

antibodies in monoclonals included:

and specific), and

samples of peach and plum to be tested identification

collected in of the (17), (21), Egypt

(SO), and of isolates (87)

peach (46), plum (37) coming the above six that they

belong to isolates (139), (20), EA (7) and to of +

Albania. et

(1996), was identified in plum the same and

thee plum samples

of and found.

was in was found only in one.

214 al.

Cyprus. samples (5), peach (9) and plum (7)

infected with same was

a single (Wetzel et al.,1991)..

Seven samples El County in two distant localities

only the existence of the El as suggested

by et

Greece. All 50 and 30 peach samples

findings on the of this in

and

The and identified in peach, plum and

thus in

and and of in the

et al., 1996; et al., 1997). A single diseased was infected by

in was found in the South-East

Turkey. samples and

with both and which made difficult.

investigations the isolates in et al., 1991,

and typed out ambiguous of both and

epitopes in the same isolate) was et al. (1998) with a peach isolate of

The combined use in one and

to be

efficient the simple, detection and isolates.

O., and

and of a monoclonal antibody specific to the

of plum pox Journal 103: 477-480.

O., and (1998). di un

monoclonale specifico il ceppo del della del susino isolato da Atti del Convegno Nazionale del Ciliegio, 19-21 giugno 1997,

Valenzano 141-145.

T., L. and

techniques de du plum pox Bulletin

Bulletin, 24: 651-656.

E., E., J.J.,

VELA, C. and A. S ~(1994). Z of plum pox using monoclonal

antibodies to and Bulletin Bulletin, 24: 569-577.

Detection and seratyping of plunz pox isolates by nzonoclonal antibodies 215

LANNEAU, and J.

Analysis of plum pox and development of assays. Acta Horticulturae, 386: 357-369.

G., LANNEAU, L.,

and J. (1994). of plum pox and analysis of its

using Bulletin Bulletin, 24: 585-594.

C A ” A , S., F.,

and J. (1998). of

Antibodies and the Typing of

to and of 88 (3): 198-203.

and E. (1997). La individuazione di isolati

del in Fitopatologico, 7-8: 61.63.

and V. (1998). status of stone in Options

Stone and in the

and (this volume).

J., and

(1998). A assay combined with analysis detection and

of plum pox isolates. Acta Horh’culturae, 472 (2): 483-491.

W. and V. (1996). account of the

status of in Lebanon. 26: 161-166.

O., T., and V. (1998).

of a monoclonal antibody specific to the El of Acta Virologica, 42: 248- 250.

L. and A. (1997). chain detection of plum pox

of

Symposium on of June 53p.

G. and of isolates of plum pox

24: 615-624.

L., G N N , E. and (1996).

di un del in coltivazioni di pesco. Agrario, 3 2 77-79.

A.S. and pox situation in Bulletin Bulletin, 24:

515-525.

C. and (1998). Application of the technique plum

pox detection field conditions in and assays to simplify techniques. Acta Horticulturae, 472 (2): 475-483.

and J. (1991). A chain

assay adapted to plum pox detection. Journal of Virological 33: 355-365.