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Serological characterisation of Prunus necrotic ringspot virus (PNRSV) isolates by monoclonal antibodies Di Terlizzi B., Boscia D., Savino V. in

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Serological characterisation of Prunus necrotic ringspot virus (PNRSV) isolates by monoclonal antibodies

Di Terlizzi B., Boscia D., Savino V.

in

Di Terlizzi B. (ed.), Myrta A. (ed.), Savino V. (ed.).

Stone fruit viruses and certification in the Mediterranean countries: problems and prospects

Bari : CIHEAM

Options Méditerranéennes : Série B. Etudes et Recherches; n. 19 1998

pages 225-227

Article available on lin e / Article dispon ible en lign e à l’adresse :

--- http://om.ciheam.org/article.php?ID PD F=99001756

--- To cite th is article / Pou r citer cet article

--- D i Terlizzi B., Boscia D ., Savino V. Serological ch aracterisation of Pru n u s n ecrotic rin gspot viru s ( PN R SV) isolates by mon oclon al an tibodies. In : D i Terlizzi B. (ed.), Myrta A. (ed.), Savino V.

(ed.). Stone fruit viruses and certification in the Mediterranean countries: problems and prospects. Bari : CIHEAM, 1998. p. 225-227 (Options Méditerranéennes : Série B. Etudes et Recherches; n. 19) ---

http://www.ciheam.org/

http://om.ciheam.org/

(2)

Biagio

Valemano,

Vito

di Studio del sui e le

delle and

di delle

dalle of

of Lija

Antakya,

AVFA de Tunis (Tunisia)

Arben

of and Food

de

Libanais

of

Section Nicosia

Christina

Athens

Vicente

-

of

the

genus posses a

genome and (25-30 to shaped The

was common and

in

stone 1988;

1992).

was phylogenetically to and apple mosaic

and and exist between

and (Fulton, 1968; 1973;

et al.,

and

Options Méditerranéennes, Série B / n 19, - Stone fruit viruses and certification in the Mediterranean: problems and prospects

CIHEAM - Options Mediterraneennes

(3)

226 B. Di Terlizzi et al.

of and

and some sufficiently distinct to be called et al., 1978;

and among the isolates was high and the bases

specific biological 1992).

So have been the most method detecting

was gel double-diffusion test and polyclonal (Fulton and 1960). et al. (1987) used to identify distinct sweet

is now the method of

choise in the detection et al. et al. and

Aichele, 1984; Uyemoto

et

al., antibodies have been to et al., 1984; et

al., 1984).

to a peach isolate and used, at the

to

investigate the of the tests to the

against isolates identified 17 et al., 1998).

detection and

of as

by

using monoclonal antibodies. samples of stone species identified with a as

et al. (1998). In total, 78 isolates collected and

against 10 isolates and locations Albania (3),

(lo), (5), (28), Lebanon (6), (5), Tunisia (7), (7) and Spain (1). In

addition, included as testing

isolates, out at the showed high

among 78 isolates tested),

on et al., 1998). These not

with host symptom

(1988). of

et al. Eds.). 17-19.

and detection and of

in by enzyme-linked assay.

A m a l e s Applied Biology, 90: 395-399.

A., O., C. and V. (1998). Uso di monoclonali

la

diagnosi di del ciliegio. h : Atti del Convegno Nazionale del Ciliegio, 19-21 giugno 1997, Valenzano 577-583.

(1973). of and apple mosaic

isolates 63: 238-240.

CIHEAM - Options Mediterraneennes

(4)

and detection of plum pox and

in woody plants by enzyme-linked Acta

Horticulturae, 6 7 51-57.

and among

82: 200-206.

FULTON, of Causing

and Apple 58: 635-638.

and of and

50: 635.

E.L., and J. of monoclonal antibodies against

and alfalfa mosaic and use in 74: 367-372.

and Epitope specificity of seven monoclonal antibodies to

apple mosaic 74: 808

Advances in Vol. 9: 261-281.

and of

in seed and seedlings by enzyme-linked assay. Disease, 68:

378-381.

A. and S. of

isolated mosaic-diseased sweet in Washington. Disease, 71:

91-93.

and in the

nucleotide sequence of Archives Virology, 142: 749-763.

and and of an of

Apple in the USA. J o t m a l 139: 33-47.

,

J.A. and of

in young peach in Disease, 73: 217-220.

CIHEAM - Options Mediterraneennes

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