1
1 T
ABLE OF
C
ONTENTS
2 INTRODUCTION... 3
2.1 The skin ... 3
2.1.1 Role and Structure ... 3
2.2 The interfollicular epidermis ... 4
2.2.1 Structure ... 4
2.2.2 Embryonic development and stratification ... 6
2.3 Modelling stem cell fate decision in epidermis ... 15
2.4 The post-natal development ... 20
2.5 The wound repair ... 21
3 OPEN QUESTIONS ... 29
4 RESULTS ... 30
4.1 Defining stem cell dynamics and migration during wound healing in mouse skin epidermis ... 30
4.1.1 Focus ... 30
4.1.2 Methods and results ... 31
4.1.3 Conclusion ... 32
4.2 Defining the design principles of skin epidermis postnatal growth ... 67
4.2.1 Focus ... 67
4.2.2 Methods and results ... 67
4.2.3 Conclusion ... 70
5 DISCUSSION ... 153
5.1 Defining stem cell dynamics and migration during wound healing in mouse skin epidermis ... 153
5.1.1 Compartmentalization during wound healing ... 153
5.1.2 Wound healing signature ... 154
5.1.3 Proliferation and migration during wound healing ... 158
5.1.4 Clonal dynamics during wound healing ... 161
5.2 Defining the design principles of Skin epidermis postnatal growth ... 166
5.2.1 Clonal dynamics during postnatal development ... 166
5.2.2 Transcriptional profiling of epidermal progenitors... 170
5.2.3 Cell division orientation and collagen fibers ... 176
6 PERSPECTIVES ... 177
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6.2 Post-natal development ... 182 7 CONCLUSION ... 186 8 REFERENCES ... 188
