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Development of SNPs markers (single nucleotide polymorphisms) presents in regulatory regions and genotyping of mapping population Hevea brasiliensis

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Academic year: 2021

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Acknowledgements: Financial support:

Development of SNPs markers (Single Nucleotide Polymorphisms)

presents in regulatory regions and genotyping of

mapping population Hevea brasiliensis

Andreotti, IAA1*; Souza, LM1; Toleto-Silva, G1; Cardoso-Silva, CB1; Conson, ARO1; Mantello, CC1; Silva, CC1; Le Guen, V2; Souza, AP1,3.

1 Molecular Biology Center and Genetic Engineering, UNICAMP, Campinas, SP, Brazil, 2 CIRAD, UMR AGAP, Montpellier, Hérault, France

3 Department of Plant Biology – Biology Institute and Molecular Biology Center and Genetic Engineering, UNICAMP, Campinas, SP, Brazil

isaandreotti@hotmail.com

 Identification of SNP allelic: KASPAR chemistry

 Freebayes detected 59,267 putative SNPs: 39,455

transitions (ts) and 19,812 transversions (tv) → Ts/Tv=1,99

 Were identified 19,118 polymorphic positions in the GT1

and 25,360 polymorphic positions in the RRIM701, of which a total of 14,568 putative SNPs was shared between cultivars.

 27 putative TFBS different were found by the program

ALGGEN-PROMO, where highlight MYB2, HSF1 and Alfin1 as the most frequent, respectively (figure below).

The rubber tree (Hevea spp.), primary plant used for natural rubber production, have a long breeding cycle. However, the advent of molecular genetic markers favored early assessment methods, allowing the characterization of genotypes, genetic diversity analysis, QTL analysis and identifying genes of interest. This project aims to develop and genotype new SNP markers presents/or near in putative transcription factor binding sites (TFBS) to two genetic mapping populations of rubber tree obtained from the crossing between RRIM701xGT1 and PB235xGT1 clones.

The transcription factors are involved in the response to biotic and abiotic stresses and your study allow increase knowledge of the mesh of responses that plants use to defend environmental stresses. The results show SNP markers presents/or near in putative transcription factor binding sites (TFBS) genotiped with success in two populations of mapping.

RESULTS AND DISCUSSION

MATERIAL AND METHODS

CONCLUSION

INTRODUCTION

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