HAL Id: hal-02800315
https://hal.inrae.fr/hal-02800315
Submitted on 5 Jun 2020
HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.
L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Sélection d’agents de lutte biologique efficaces pour lutter contre la fusariose vasculaire du cyclamen
Charline Lecomte, Hamida Bouksil, Veronique Edel-Hermann, Agnès Langlois, Fabien Robert, Christian Steinberg, Claude Alabouvette
To cite this version:
Charline Lecomte, Hamida Bouksil, Veronique Edel-Hermann, Agnès Langlois, Fabien Robert, et al..
Sélection d’agents de lutte biologique efficaces pour lutter contre la fusariose vasculaire du cyclamen.
5. Conférence Internationale sur les Méthodes Alternatives de Protection des Plantes AFPP, Mar 2015, Lille, France. 1 p., 2015. �hal-02800315�
Screening for efficient biocontrol microorganisms to manage fusarium wilt of cyclamen
Lecomte C.1,2, Bouksil H.2, Edel-Hermann V.2, Langlois A.1, Robert F.1, Steinberg C.2, Alabouvette C.3 E-mail : charline.lecomte@dijon.inra.fr
1 ASTREDHOR, 44, rue d’Alésia, 75682 Paris, France; 2 INRA Dijon, UMR 1347 Agroécologie, 17 rue Sully, 21065 Dijon, France; 3 Agrene, 47 rue C. Pierrot, 21000
OBJECTIVES
METHODS INTRODUCTION
Fusarium wilt of cyclamen
0 Fusarium oxysporum f. sp. cyclaminis (Focy)-Tracheomycosis
0 The most destructive disease on cyclamen 0 Up to more than 50 % losses
RESULTS
CONCLUSION
0 Design of a specific molecular marker for early detection of the pathogen 0 Selection of one or several efficient BioControl Agents (BCA)
BCA tested
0 Marketed products but without allegation to control Focy
0 7 products
0 Tested alone or in combination 0 Mode of action evaluated in vitro
The Focy strain
0 A strain (MIAE01507) isolated from a cyclamen seedling in 2009 and known as highly aggressive
0 15 inoculated plants/treatment
Product number Type of microorganisms
1 Fungus
2 Bacteria
3 Mycorhizal fungus 4 Fungus and bacteria
5 Fungus
6 Fungus
7 Fungus
Biocontrol test
0 Post sowing problems
A Seedling emergence, phytotoxicity, Botrytis development
A Not enough plants available: ratio 1/1 not tested*
0 Efficient products?
A Only tendances
A Phytotoxicity or slow down emergence effect: 3, 6
A Inefficient products: 4, 5, all combinations
A Selected for further tests: 1, 2, 7
BCA’s modes of action
0 Several modes of action highlighted:
A Antibiosis: 2
A Mycoparasitism: 4, 5, 7
0%
10%
20%
30%
40%
50%
60%
70%
80%
Percentage of dead plants
0
* * * * *
104 105 106
ACKNOWLEDGEMENTS
0 Some products showed a phytotoxic effect on cyclamen
0 Three promising products identified and selected for further tests 0 Microorganisms showed various modes of action against Focy
Nadine Gautheron • Marc-Antoine Cannesan • Yohann Daguerre • Sébastien Terrat
Mars 2015 • Crédits photos : ASTREDHOR Prophylactic measures
Highly recommended
Varietal breeding Nonexistant
Chemical No curative control
Biocontrol
Promising results
12 weeks 1 week 11 weeks
Cyclamen sowing
106 cfu*
of product/L substrate
106 cfu of product/L
106 , 105, and 104 cfu of pathogen/L substrate
106 , 105, and 104 cfu of pathogen/L substrate
Number
of dead plants/treatment 3-4 leaves seedlings transplanting 1st Focy inoculation 2nd Focy inoculation Disease incidence assessment
INRA 2012
No efficient and eco-friendly
solution
*cfu: colony forming unit
3 Focy/BCA ratios tested: 1/1, 1/10, 1/100
Concentration of Focy (cfu/L of substrate
