HAL Id: hal-02821070
https://hal.inrae.fr/hal-02821070
Submitted on 6 Jun 2020
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Hen oocyte quality and fertility
Sébastien Elis, Marina Govoroun, Joëlle Dupont, Philippe Monget, Isabelle Couty, Elisabeth Blesbois, Florence Batellier
To cite this version:
Sébastien Elis, Marina Govoroun, Joëlle Dupont, Philippe Monget, Isabelle Couty, et al.. Hen oocyte quality and fertility. International Chick Meeting, Apr 2007, Barcelone, Spain. 1 p., 2007. �hal- 02821070�
Hen oocyte quality and fertility
S. Elis, M. Govoroun, J. Dupont, P. Monget, I. Couty, E. Blesbois, F. Batellier
INRA, PRC – 37380 Nouzilly
The reproductive physiology of the hen shows numerous specificities that include oviparity and telolecithe oocyte. Moreover, the intensive selection on conformation parameters leads to a dramatic decrease in reproductive
ability. This leads to investigate the regulation of the expression and function of genes involved in the maturation of the oocyte, with
consequences on the development of the early embryo. This last point is very important in hen because of the late embryonic genome reactivation.
The aim of the present study was to explore the expression of two genes
of the TGF- b family suggested to be implicated in fertility, gdf9 and bmp15. Biological effects of BMP15 were also
investigated.
Introduction Materials and Methods
mRNA was extracted from Granulosa Cells, Germinal Disc Region and embryo with tri-reagent for quantitative RT PCR on candidate genes.
Tissular specificity and pattern of expression were explored during follicular maturation and early embryo development.
In situ hybridization was performed on hen ovary to localize these genes.
Effects of one of these genes, BMP15, were investigated on granulosa cells of F3/4, F2 and F1 follicles
GCs
Granulosa Cells
Granulosa layer
Perivitelline membrane
Germinal Disc GD
GDR: Germinal Disc Region
= GD + GCs
bmp15 and gdf9 are
expressed in the hen oocyte Their expression decreased after the ovulation, even after
the embryonic genome
activation. They should be used during the follicular maturation
or early embryo development BMP15 inhibits progesterone
induced secretion
BMP15 may have a key role in the female fertility in birds
Preferentially expressed in the ovary
GDF9 : immature ovary
BMP15 : mature ovary Sense probe
100µm
BMP15, 200 IGF-1 LH
BMP15, 50
FSH
B. F2
abc
e
0 5 10 15 20 25 30 35
ab bc ab
abc abc
d
a
(ng/ml/300, 000 cells)
progesterone
C. F1
a be
abe ae d bd
c
be abe
0 20 40 60 80 100 120
BMP15, 200 IGF-1 LH
BMP15, 50
FSH
(ng/ml/300, 000 cells)
progesterone
BMP15, 200 IGF-1 LH
BMP15, 50 FSH
(ng/ml/300, 000 cells)
progesterone
A. F3/4
b ab a
d
c ab ab ab ab
0 4 8 12 16 20
C. BMP15
Ratio BMP15/EF1
0 0.01 0.02 0.03 0.04 0.05 0.06 0.07
0 6.5 12 24
a
b
c
cd
d d
Time after the ovulation (h)
36 48
c c bc
b
c a
0 0.1 0.2 0.3 0.4 0.5 0.6
Ratio GDF9/EF1 (x10-3 )
D. GDF9
0 6.5 12 24
Time after the ovulation (h)
36 48
A. BMP15
F6 F5 F4 F3 F2 F1 F6 F5 F4 F3 F2 F1
Granulosa cells Germinal Disc Region
Ratio BMP15/EF1
0 0.1 0.2 0.3 0.4
a
bc
a ab ab ab a
c bc c c
d
bc c
bc
B.GDF9
Ratio GDF9/EF1 (x10-3 )
0 0.5 1 1.5
2 2.5
F6 F5 F4 F3 F2 F1 F6 F5 F4 F3 F2 F1
Granulosa cells
b
abc
a a a e
d
c c c ac b
Germinal Disc Region
727 bp 757 bp
a a a a a a a a a a a
GDF9
0 2000 4000 6000
O Sp I G L H S B M Lu Hy C-
b
Bmp15
0 100 200 300 400
O Sp I G L H S B M Lu Hy C-
a a a a a a a a a a a b
