Estrogen-induced changes in the hepatic metabolism of plasma lipoproteins in the pre-ruminant calf

(1)

HAL Id: hal-00888820

https://hal.archives-ouvertes.fr/hal-00888820

Submitted on 1 Jan 1992

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Estrogen-induced changes in the hepatic metabolism of plasma lipoproteins in the pre-ruminant calf

S Auboiron, Denys Durand, J Lefaivre, Dominique Bauchart, Mj Chapman

To cite this version:

S Auboiron, Denys Durand, J Lefaivre, Dominique Bauchart, Mj Chapman. Estrogen-induced changes

in the hepatic metabolism of plasma lipoproteins in the pre-ruminant calf. Annales de zootechnie,

INRA/EDP Sciences, 1992, 41 (1), pp.117-117. �hal-00888820�

(2)

Estrogen-induced changes ⁱⁿ ^the hepatic ^metabolism

of plasma lipoproteins ^{in the} pre-ruminant ^calf

S Auboiron D Durand J Lefaivre D Bauchart MJ Chapman 3

1

INRA Theix, Unité de Recherche des Métabolismes Energétique

^et

Lipidique;

3

2 Unité Dynamique ^de Digestion, ⁶³¹²² Saint-Genès-Champanelle;

3 I

NSERM _U321, Unité de Recherche

sur

les Lipoprotéines ^et l a thérogénèse, Hôpital ^{de la} Pitié, ⁷⁵⁶⁵¹ ^Paris Cedex, ^France

The hepatic synthesis and secretion of

lipoproteins ^such

^as

VLDL and HDL is

particularly important in lipoprotein

homeostasis in bovine species (Bau-

chart et al, 1989). ^{On the}

^one

hand,

VLDL-mediated hepatic triglyceride

^ex-

port ^{is low} ^and

^can

^be limiting during lipomobilization, ^while

^on

^{the other} hand, HDL, ^which ^accounts ^for

^more

than 75% of total plasma lipoproteins, plays

^a

major ^role ⁱⁿ

^reverse

cholesterol

transport ⁱⁿ ^these species. ^In ^order ^to

stimulate the hepatic production ^of lipo- proteins, estrogen

^was

administered to three 3-week-old male Friesian calves

(55 ± 3 kg body weight) equipped ^with

catheters and electromagnetic ^flow probes for estimation of hepatic lipo- protein fluxes. Animals

were

fed

a con-

ventional milk replacer ^{in which} lipid

and carbohydrate ^content

^was

^reduced

to 30% in order to induce lipomobiliza-

tion. 17 I3-Estradiol (E)

^was

then admin- istered by ^infusion through ^the portal

route (20 pg per kg BW) ^{for 1 h} ^after

the morning ^{meal and} subsequently by

intramuscular injection (200 pg/d per

kg BW)

^over

^the following ⁴ ^{d. Blood} samples

^were

^collected ⁷ ^h ^{after the} morning meal. Total plasma lipoproteins

(d

^<

^1.180 g/ml)

^were

separated by

density-gradient ultracentrifugation ^into

22 subfractions and their respective

chemical composition

^was

^determined by enzymatic ^and immunological

methods.

Estrogen ^treatment ^did ^not signific- antly modify ^the physicochemical prop- erties and density distribution of bovine plasma lipoproteins. ^In spite of restric- tive energy supply, low levels of hepatic

VLDL production

^were

^observed ^under

control conditions (0.09 mg/min per kg BW). Hepatic ^VLDL production

^was

stimulated by estrogen perfusion (EP) (0.32 mg/min per kg BW) ^but strongly

reduced by ^the long-term ^treatment ^of estrogen administered by intramuscular

injection (EI) (-0.62 mg/min per kg BW); ^these findings emphasize ^{the im-} portance ^{of the} ^route of administration and the hormonal dose under

our

di- etary conditions. Estrogen ^treatment

stimulated hepatic production ^of light

HDL (1.060 - 1.091 g/ml) (- 2.71

^vs

1.17 and 2.16 mg/min per kg ^{BW in} control, ÊP ând Êl ^treatment respec-

tively) ^and hepatic uptake ^of heavy ^HDL (1.091 - ^1.180 g/ml) (2.35

^{vs -}

^0.52

and 0.13 mg/min per kg BW). ^Modifica-

tion in the hepatic metabolism of HDL

by estrogen may involve

^a

decrease in

hepatic lipase activity (Hazzard ^et al, 1984) ^and cholesterol esterification by LCAT.

Bauchart D, ^Durand D, Laplaud ^PM, Forgez P, Goulinet S, Chapman ^MJ (1989) J Lipid

Res 30,

1499-1513 3

Estrogen-induced changes in the hepatic metabolism of plasma lipoproteins in the pre-ruminant calf

HAL Id: hal-00888820

https://hal.archives-ouvertes.fr/hal-00888820

Submitted on 1 Jan 1992

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Estrogen-induced changes in the hepatic metabolism of plasma lipoproteins in the pre-ruminant calf

S Auboiron, Denys Durand, J Lefaivre, Dominique Bauchart, Mj Chapman

To cite this version:

S Auboiron, Denys Durand, J Lefaivre, Dominique Bauchart, Mj Chapman. Estrogen-induced changes

in the hepatic metabolism of plasma lipoproteins in the pre-ruminant calf. Annales de zootechnie,

INRA/EDP Sciences, 1992, 41 (1), pp.117-117. �hal-00888820�

Estrogen-induced changes in the hepatic metabolism

of plasma lipoproteins in the pre-ruminant calf

S Auboiron D Durand J Lefaivre D Bauchart MJ Chapman 3

INRA Theix, Unité de Recherche des Métabolismes Energétique

Lipidique;

2 Unité Dynamique de Digestion, 63122 Saint-Genès-Champanelle;

3 I

NSERM U321, Unité de Recherche

les Lipoprotéines et l a thérogénèse, Hôpital de la Pitié, 75651 Paris Cedex, France

The hepatic synthesis and secretion of

lipoproteins such

VLDL and HDL is

particularly important in lipoprotein

homeostasis in bovine species (Bau-

chart et al, 1989). On the

hand,

VLDL-mediated hepatic triglyceride

port is low and

be limiting during lipomobilization, while

the other hand, HDL, which accounts for

than 75% of total plasma lipoproteins, plays

major role in

cholesterol

transport in these species. In order to

stimulate the hepatic production of lipo- proteins, estrogen

administered to three 3-week-old male Friesian calves

(55 ± 3 kg body weight) equipped with

catheters and electromagnetic flow probes for estimation of hepatic lipo- protein fluxes. Animals

fed

ventional milk replacer in which lipid

and carbohydrate content

reduced

to 30% in order to induce lipomobiliza-

tion. 17 I3-Estradiol (E)

then admin- istered by infusion through the portal

route (20 pg per kg BW) for 1 h after

the morning meal and subsequently by

intramuscular injection (200 pg/d per

kg BW)

the following 4 d. Blood samples

collected 7 h after the morning meal. Total plasma lipoproteins

(d

1.180 g/ml)

separated by

density-gradient ultracentrifugation into

22 subfractions and their respective

chemical composition

determined by enzymatic and immunological

methods.

Estrogen treatment did not signific- antly modify the physicochemical prop- erties and density distribution of bovine plasma lipoproteins. In spite of restric- tive energy supply, low levels of hepatic

VLDL production

observed under

control conditions (0.09 mg/min per kg BW). Hepatic VLDL production

stimulated by estrogen perfusion (EP) (0.32 mg/min per kg BW) but strongly

reduced by the long-term treatment of estrogen administered by intramuscular

injection (EI) (-0.62 mg/min per kg BW); these findings emphasize the im- portance of the route of administration and the hormonal dose under

di- etary conditions. Estrogen treatment

stimulated hepatic production of light

HDL (1.060 - 1.091 g/ml) (- 2.71

1.17 and 2.16 mg/min per kg BW in control, EP and El treatment respec-

tively) and hepatic uptake of heavy HDL (1.091 - 1.180 g/ml) (2.35

0.52

and 0.13 mg/min per kg BW). Modifica-

tion in the hepatic metabolism of HDL

by estrogen may involve

decrease in

hepatic lipase activity (Hazzard et al, 1984) and cholesterol esterification by LCAT.

Bauchart D, Durand D, Laplaud PM, Forgez P, Goulinet S, Chapman MJ (1989) J Lipid

Estrogen-induced changes ⁱⁿ ^the hepatic ^metabolism

of plasma lipoproteins ^{in the} pre-ruminant ^calf

2 Unité Dynamique ^de Digestion, ⁶³¹²² Saint-Genès-Champanelle;

NSERM _U321, Unité de Recherche

les Lipoprotéines ^et l a thérogénèse, Hôpital ^{de la} Pitié, ⁷⁵⁶⁵¹ ^Paris Cedex, ^France

lipoproteins ^such

chart et al, 1989). ^{On the}

port ^{is low} ^and

^be limiting during lipomobilization, ^while

^{the other} hand, HDL, ^which ^accounts ^for

major ^role ⁱⁿ

transport ⁱⁿ ^these species. ^In ^order ^to

stimulate the hepatic production ^of lipo- proteins, estrogen

(55 ± 3 kg body weight) equipped ^with

catheters and electromagnetic ^flow probes for estimation of hepatic lipo- protein fluxes. Animals

ventional milk replacer ^{in which} lipid

and carbohydrate ^content

^reduced

then admin- istered by ^infusion through ^the portal

route (20 pg per kg BW) ^{for 1 h} ^after

the morning ^{meal and} subsequently by

^the following ⁴ ^{d. Blood} samples

^collected ⁷ ^h ^{after the} morning meal. Total plasma lipoproteins

^1.180 g/ml)

density-gradient ultracentrifugation ^into

^determined by enzymatic ^and immunological

Estrogen ^treatment ^did ^not signific- antly modify ^the physicochemical prop- erties and density distribution of bovine plasma lipoproteins. ^In spite of restric- tive energy supply, low levels of hepatic

^observed ^under

control conditions (0.09 mg/min per kg BW). Hepatic ^VLDL production

stimulated by estrogen perfusion (EP) (0.32 mg/min per kg BW) ^but strongly

reduced by ^the long-term ^treatment ^of estrogen administered by intramuscular

injection (EI) (-0.62 mg/min per kg BW); ^these findings emphasize ^{the im-} portance ^{of the} ^route of administration and the hormonal dose under

di- etary conditions. Estrogen ^treatment

stimulated hepatic production ^of light

1.17 and 2.16 mg/min per kg ^{BW in} control, ÊP ând Êl ^treatment respec-

tively) ^and hepatic uptake ^of heavy ^HDL (1.091 - ^1.180 g/ml) (2.35

^0.52

and 0.13 mg/min per kg BW). ^Modifica-

hepatic lipase activity (Hazzard ^et al, 1984) ^and cholesterol esterification by LCAT.

Bauchart D, ^Durand D, Laplaud ^PM, Forgez P, Goulinet S, Chapman ^MJ (1989) J Lipid

Hazzard WR, Haffner SM, ^Kushwaha RS, Applebaum-Bowden ^D, ^{Foster DM} (1984)