Mattheu Chabannes
CIRAD Département BIOS
Eva Marazuela
Fundación PROMIVA
Marisé Borja
Universidad Complutense de Madrid/Fundación PROMIVA
Expression of an
Expression of an Arabidopsis Arabidopsis Aspartic protease
Aspartic protease in in Pelargonium Pelargonium
CRISP Project HPRN-CT-2000-00093
Phenotypic alterations of over-expressing CDS10 Arabidopsis plants
Wt plants CDS10 plants
Smaller rosette
Smaller and
asymmetric leaves
Two independent CDS10 flowering plants
Bushy, multi-branching phenotype
Plants are always dwarf (10 cm high maximum)
Pelargonium Boda Gitana Salmon biolistic transformation
Embryogenesis regeneration
Pelargonium Boda Gitana Salmon biolistic transformation
NPTII selection
CDS10-Boda Gitana Salmon
Bushy, multi-branching phenotype is observed in vitro
Boda Gitana Salmon plants
Wt CDS10 over-expression
Bushy, multi-branching phenotype is observed ex vitro
Boda Gitana Salmon leaves
Wt CDS10 over-expression
- No variations are
observed in the shape and size of the leaves - Peduncles are longer and thinner
Boda Gitana Salmon flowers
Wt CDS10 over-expression
- Number of petals is reduced to 5 like in simple varieties
Pelargonium biolistic transformation
Mirada Pink Mirada Violet
Pelargonium biolistic transformation
Mirada Pink Mirada Violet
Pelargonium Mirada violet biolistic transformation
NPTII selection
Pelargonium Mirada violet
Wt CDS10 over-expression
Pelargonium Mirada Pink
Wt CDS10 over-expression
Pelargonium Mirada Pink-Wild Type
Pelargonium Mirada Pink-
CDS10 over-expression
Pelargonium Mirada Violet
CDS10 over-expression
Mirada Pink and Violet flowers
Wt CDS10 over-expression Wt CDS10 over-expression
Mirada Pink Mirada Violet
- Number of petals is reduced to 5 in both varieties
49.8 5
12.31 T-7
51.3 5
4.86 Wt
Mirada Pink
49.6 5
11.71 T-22
53.2 12.32
4.36 Wt
Mirada Violet
67.2 13.39
9.6 T-140
65.2 5
10.9 T-138
63.4 5
12.10 T-147
59.1 5
15.57 T-129
56.8 5
13.00 T-131
71.3 21.66
5.57 Wt
Boda Gitana Salmon
height (cm) Number
of petals Number of
branches Line
Wild type vs CDS10 overexpressing lines
Conclusions
+ The over expression of At -CDS10 in Pelargonium transgenic lines induces an increase in the number of branches, longer
pedicels and a reduction in plant height.
+ At-CDS-10 over expression also induces the decrease in the number of petals in two double flowered varieties transgenic plants .
+ Even if the original A. thaliana busy phenotype was of interest for getting a higher number of cuttings/plant and a compact
phenotype, the pleiotropic effects of the over-expression of the CDS 10 gene on the flowers are non favourable in the case of Pelargonium.
