HAL Id: inserm-00176537
https://www.hal.inserm.fr/inserm-00176537
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Phosphate is a specific signal for ATDC5 chondrocyte
maturation and apoptosis-associated mineralization:
possible implication of apoptosis in the regulation of
endochondral ossification.
David Magne, Gilles Bluteau, Corinne Faucheux, Gaby Palmer, Caroline
Vignes-Colombeix, Paul Pilet, Thierry Rouillon, Joseph Caverzasio, Pierre
Weiss, Guy Daculsi, et al.
To cite this version:
David Magne, Gilles Bluteau, Corinne Faucheux, Gaby Palmer, Caroline Vignes-Colombeix, et al.. Phosphate is a specific signal for ATDC5 chondrocyte maturation and apoptosis-associated mineraliza-tion: possible implication of apoptosis in the regulation of endochondral ossification.. Journal of Bone and Mineral Research, American Society for Bone and Mineral Research, 2003, 18 (8), pp.1430-42. �inserm-00176537�
0.5 mM PFA 0 20 40 60 80 100 µ g C a/ m g p ro t 0 50 100 150 µ g C a/ m g p ro t 4 mM Pi - + - + - + 8 H 24 H 48 H * * * 0 200 400 600 800 1000 1200 1 2 4 7 10 µ g C a/ m g p ro t Pi (mM) * * * 4 mM Pi - + - +
c
0 10 20 30 40 50 60 70 µ g C a/ m g p ro t * * 1 1.5 2.5 5 Ca (mM) 4 mM Pi - + - + - + - +d
- + - + NSa
b
maturation maturation
a
b
Wavenumbers (cm-1 ) 960 1140 Labile PO4 HPO4 2-A B S Wavenumbers (cm-1) 960 1140 A B S HPO4 2-Labile PO43-d
maturation maturationColl X GAPDH PFA 1 4 1 4 Pi (mM) -RT
b
a
Pi MW 0 1h 2h 24h 48h Coll X (583 pb) OPN (239 pb) 30’ GAPDH (418 pb) 4 mM 1 mM 4 mM 1 mM 1 mM 4 mM -RT 0 20 40 60 80 100 120 µ g C a/ m g p ro t 2.4 mM Ca * CHX 4 mM Pi - - + + - - + + - + - +c
nodule top bottom 0 20 40 60 80 100 % M T S a ct iv it y 4 mM Pi 2.4 mM Ca 5 µM CHX - + - - + + - - + - + - - - + - + * * ** 0 20 40 60 80 100 120 % M T S a ct iv it y 4 mM Pi + 2.4 mM Ca PFA - - + + - + - + **
b
+ *0 0,2 0,4 0,6 0,8 1 1,2 CT 4 mM Pi 2.4 mM Ca 4 mM Pi + 2.4 mM Ca b cl -2 /b a x (c o rr e ct ed f o r G A P D H ) Bax Bcl2 GAPDH 2.4 mM Ca 4 mM Pi MW -RT - + - + - - + + 175 pb 215 pb 418 pb
