Risk assessment of spreading Banana streak viruses through large scale distribution of banana interspecific hybrids

(1)

RISK ASSESSMENT OF SPREADING

· :-1

~

BANANA STREAK V/RUSES

(BSV)

U

.

reurope

THROUGH LARGE SCALE DISTRIBUTION

~

,..,.1

s

~

OF BANANA INTERSPECIFIC HVBRIDS

E

li

sa Javer Higg

inson

'

, Reina Teresa Martinez

l

,

M

.

D.L.

R

eyes

3,

A. Ba

r

ahona

,

M.A.

Fonseca

3

R.M Garz6n

3,

N. Arencibia

3

,

R. Chi

nea

• &

Pierre Yves Teycheney'

'

I

N

ISAV ejave

r @inisav

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cu

,

2

IDIAF, rmartinez@

idiaf.gov.do

,

3

_LAPROSAVS

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_Cuba

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_{LCCV, Cuba}

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_{RAD, Guade}

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9. ...

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...

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Musa b

albisiana

spp host infectious

endogenous

Banana streak virus (eBSV)

..

.

-

....

••

•

• _•

• •

_•

• _•

Viral particles

lnfectious eBSV integrated

~

. . into the genome of the

j

'

_Activating

M. balbisiana parent stress

taeld survey & sample collection

• FHIA 21 (AAABl, MxH (AAB) (DR) FHIA 18 (AAAB .CEMSA % (AAB) {Cuba)

Mealybug vector species present

Agronomical data

• ••

.,

.

Ol,GF lM and MYspBSV forPCR -IC-Mbylndexing

IC30 RT stresses:

• .

.

• interspecifie crosses • temperature differences • micropropagation

S

tudyi

n

g t

h

e

levels of prevalence and molecular

divers

ity of BS

V can provide a hint into the risk of

sprea

ding

BSV

s

through

large

scale distribution

of interspecific

hybrid

s

Cuba and the Dom

in

i

can Re

p

ublic are

1 1 1 1

YYYY

Ti

l

pnmers SSV • specifie

Monkeys

+

primers

important producers

h

ybr

i

ds

in

Coating 0/N 4C0 ~ ~

t

DNAsel Multiplex- PCR ~

...

1!1 eBSGFV-7 VI al

"'

::;

eBSGFV-9

the Car

i

b

ean

--

-

-• •-•~ lnfectious

1 • a .tH H t- . Non-lnfectious PCR markers

...

-

•

• -

te

-

•

lnfectious

_e

0 VI al

"'

eBSOIV-1 eBSOIV-2 ut • .,_ - - Non-lnfectious ~ - Alle w

>

eBSimV-1

m

--

H

i

tt

tl

• --•

-

·

· -

lnfectious o 111 ~~ lnfectious

~

eBSimV-2

eBSVs patterns of studied hybrids

• Low proportion of symptomatic

plants in both cultivars (6%)

FHIA18

CEMSA 3/4

Location

13/2

4 7/21

• Symptoms based diagnostic is not No.

reliable samoles Presence of mealybugs/ location

18/24

Infection (%) Symptomatic 40 32,5% Asymptomatic 631 2,0%

r

• The percentage of ssv infecte"tïjïian"ïsis-~erv simila;in ..

boÏÏ;

··

cultivars CEMSA 3/4 FHIA 18 .,_~._.,;.;..

....

Infection% (sucker) 3.1% 4.1% ...

__ _

lnfecction %

(in vitro culture) 5% 5.4%

No. of • 3,2% of infected plants were colonized by

identified

o

.

O.brevipes P. citri mealybugs. There is no relationship

Brevipes

+

P.minor between presence/absence mealybugs and sa moles

infection%

26 23 2 1

No. lndexing Total % eBSVs oattern sa moles B50LV BSGFV BSIMV BSMYVinfected 0 eBSOLV eBSGFV eBSimV

CEMSA3/4 248 8

0

8 3.2 Oll GF7 - - - - - -FHIA18 423 17 1 0

0

18

4.3

Oll/ GF7/ l OL2? GF9 Total 671 25

0

26 % 96,1 3,8%

0

%

1. Overalllow% of infection by BSV 2. BSOLV causes the 96,1% of ali infections 3. BSIMV and BSMYV not detected

4. FHIA 18 has both alleles for eBSGFV and eBSOLV: true AAAB? Technical problem with PCR?

Chabannes et al., (2013).

J. Virol,

doi:10.1128/JVI.00899 -13

Pseudostem splitting was the

most commonly observed

Symptoms /location symptom FHIA-21 MXH 18/21 10/21 FHIA-21 MXH Total

%

G

enotype No . 1 samp es AAAB 88 AAB 88 176 lndexing BSOLV BSIMV 22

0

1

0

26

0

100

0

-

-mealybugs

~

/location 17/21 13/21 Infection (%) 25 1.1 14.8% eBSV patterns eBSOLV eBSGFV Oll 1 GF7 OL1 1 GF7

1. A significant proportion of FHIA-21 (25%) is infected with BSOLV, the level of infection in MXH is very low (1,1%).

2. Good correlation between indexing results and eBSOLV patterns for both hybrids.

3. BSIMV not detected eBSIMV pattern has not been established yet. ....-...;_---,