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Enz yme -b ased A ssa y (Log MPN mL

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(1)

-1 0 1 2 3 4 5 6 7 8

-1 0 1 2 3 4 5 6 7 8

Enz yme -b ased A ssa y (Log MPN mL

-1

)

Culture (Log CFU mL

-1

)

Pseudalert = 0.2293+0.9843*x; 0.95 Pred.Int.

-1 0 1 2 3 4 5 6 7 8

culture -1

0 1 2 3 4 5 6 7 8

Pseudalert

y = 0.98 x + 0.23 R

2

= 0.99

Fig. S1 a)

-1 0 1 2 3 4

-1 0 1 2 3 4

Enz yme -b ased A ssa y (Log MPN mL

-1

)

Culture (Log CFU mL

-1

)

Pseudalert CL = 0.1213+0.9721*x; 0.95 Pred.Int.

-1 0 1 2 3 4

Culture CL -1

0 1 2 3 4

Pseudalert CL

y = 0.97 x + 0.12 R

2

= 0.99

b)

0 1 2 3 4

0 1 2 3 4

Enz yme -b ased A ssa y (Log MPN mL

-1

)

Culture (Log CFU mL

-1

)

Pseudalert CU = 1.741+0.3972*x; 0.95 Pred.Int.

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0

Culture CU 0.0

0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5

Pseudalert CU

c)

(2)

Fig. S1. Log transformed data for paired measurements using culture and the enzyme-based assay in absence of chlorine or copper, same data as presented in Fig. 2 (a), in presence of free chlorine (b) and copper ions (c), same data as presented in Fig. 5. Linear regression is

represented by the black line with the equation and R

2

value. Prediction interval (95%) is

represented by the dotted lines. Error bars indicate standard deviation.

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