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Opposing and uncoupling effects of mTOR and S6K1 in the regulation of endothelial tissue factor expression

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Academic year: 2022

Partager "Opposing and uncoupling effects of mTOR and S6K1 in the regulation of endothelial tissue factor expression"

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1 Supplementary material

Materials

Anti-

Į

-tubulin antibody was purchased from Sigma (#T5168); anti-p38mapk was from BD Transduction laboratories (#612168); antibodies against mTOR (#2972), phospho-S6K1 (#9205), phospho-S6 (#2211), phospho-p38mapk (#9211),

I ț B Į

(#9242) and phospho-

I ț B Į

(# 9241) were from Cell Signalling; anti-S6K1 was from Dr. G. Thomas (University of Cincinnati, USA); anti-TF was from American Diagnostica Inc. (#4503); Alexa Fluor680- conjugated anti-mouse IgG was from Molecular Probes/Invitrogen (#A21057) and IRDye800-conjugated anti-rabbit IgG was from LI-COR Biosciences (#926-32211); human TNF

Į

was from R&D systems (#210-TA-010); Thrombin from human plasma (#605195) and PD98059 was from Calbiochem; MG-132 was from Alexis; SB202190 was from Tocris;

Fluvastatin was kindly provided by Novartis AG. All cell and tissue culture media were purchased from Gibco. Endothelial cell growth supplements were from PromoCell.

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