1 Supplementary material
Materials
Anti-
Į
-tubulin antibody was purchased from Sigma (#T5168); anti-p38mapk was from BD Transduction laboratories (#612168); antibodies against mTOR (#2972), phospho-S6K1 (#9205), phospho-S6 (#2211), phospho-p38mapk (#9211),I ț B Į
(#9242) and phospho-I ț B Į
(# 9241) were from Cell Signalling; anti-S6K1 was from Dr. G. Thomas (University of Cincinnati, USA); anti-TF was from American Diagnostica Inc. (#4503); Alexa Fluor680- conjugated anti-mouse IgG was from Molecular Probes/Invitrogen (#A21057) and IRDye800-conjugated anti-rabbit IgG was from LI-COR Biosciences (#926-32211); human TNF
Į
was from R&D systems (#210-TA-010); Thrombin from human plasma (#605195) and PD98059 was from Calbiochem; MG-132 was from Alexis; SB202190 was from Tocris;Fluvastatin was kindly provided by Novartis AG. All cell and tissue culture media were purchased from Gibco. Endothelial cell growth supplements were from PromoCell.