• Replacement of an old camera for a better one.
• Tilt of the beam splitter to separate the signal from the back-reflection of the cube.
• LabVIEW sub-programs for scan, data averaging
and export. Python implementation for visualisation.
• Observation of a biological sample in water.
PIMS O.C.T. (Optical Coherent Tomography)
www.institutoptique.fr
Project carried out by Emmanuel BOURGON, Ruiyang HUANG, Zhangdi CHEN, Zijun XIAO, Hamza KHITOUS Under supervision of Nathalie WESTBROOK, Thierry AVIGNON, and Julien MOREAU
Abstract
Prospect
• Improve the stability of the setup and parasitic light elimination.
Find the optimum number of images to average (shot-noise vs mechanical stability).
• Test the performances and faisability for using during a labwork of different biological samples.
Scheme
Fig.1 OCT Scheme
The OCT is based on a Michelson interferometer but using a white lamp to have an ultra-short coherence length. The idea is to limit the interferences to a µm slice inside the sample.
Fig.2 Optical Setup
1 Köhler Illumination with an halogen lamp 2 Sampling arm with a motorized z-scan
3 Reference arm: glass slide with low reflection 4 Full-field Camera
1
2 3
4
Performance s
• Longitudinal resolution (z-axis):
4 μm in air; 3 μm in water
• Transverse resolution (xy-plane):
~1 μm /pixel
• Field of view in xy-plane:
Φ500μm
Fig.4 3D-Reconstruction of a tadpole's eye
Fig.3 xy image of a tadpole's eye
• Obtain interference fringes with a glass slide sample.
• Place the biological sample; scan the z-axis to find the upper surface. Launch automated z-scan acquisition with a given number of averaged images.
• Process xy images (en face) to reconstruct the 3D model of the sample.
Measurement p rocess
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