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A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas

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Academic year: 2021

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Figure 1. Characterization of raa1-314B and raa1-L137H. (A) Immunoblot analysis of total cell extracts from the wild-type (WT), the mutants raa1-314B and raa1-L137H, and the mutants rescued with the genomic DNA (cosmid #2), or the midigene construct
Figure 2. Expression of Raa1 in the mutants. (A) Schematic representation of the Raa1 mRNA with the fragments amplified in the RT–PCR analysis
Figure 3. Deletion analysis of Raa1. (A) Immunoblot analysis with anti-HA antibodies of total cell extracts from transformants of raa1-L137H (lanes 1–10) or raa1- raa1-314B (lanes 11–16) with the midigene constructs depicted in panel C
Figure 5. Raa1 is part of a large RNase sensitive complex. (A) Subcellular localization of Raa1

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