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Shock and Enhanced Thermal Regime to Control the Growth of a Persistent Legionella pneumophila Strain

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Titre:

Title:

Combination of heat shock and enhanced thermal regime to control the growth of a persistent Legionella pneumophila strain:

suppléments Auteurs:

Authors: Émilie Bédard, Inès Boppe, Serge Kouame, Philippe Martin, Linda Pinsonneault, Louis Valiquette, Jules Racine et Michèle Prévost Date: 2016

Type: Article de revue / Journal article

Référence:

Citation:

Bédard, É., Boppe, I., Kouame, S., Martin, P., Pinsonneault, L., Valiquette, L., ...

Prévost, M. (2016). Combination of heat shock and enhanced thermal regime to control the growth of a persistent Legionella pneumophila strain. Pathogens, 5(2). doi:10.3390/pathogens5020035

Document en libre accès dans PolyPublie

Open Access document in PolyPublie

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PolyPublie URL: https://publications.polymtl.ca/3603/

Version: Matériel supplémentaire / Supplementary material Révisé par les pairs / Refereed Conditions d’utilisation:

Terms of Use: CC BY

Document publié chez l’éditeur officiel Document issued by the official publisher

Titre de la revue:

Journal Title: Pathogens (vol. 5, no 2) Maison d’édition:

Publisher: MDPI URL officiel:

Official URL: https://doi.org/10.3390/pathogens5020035 Mention légale:

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Supplementary Materials: Combination of Heat

Shock and Enhanced Thermal Regime to Control the Growth of a Persistent Legionella pneumophila Strain

Emilie Bédard, Inès Boppe, Serge Kouamé, Philippe Martin, Linda Pinsonneault, Louis Valiquette, Jules Racine and Michèle Prévost

Figure S1. Temperature measured at the water heater outlet (blue) and on the return loop prior to the water heater (orange) for systems A and B.

30 35 40 45 50 55 60 65

Temperature (°C)

Date

30 35 40 45 50 55 60 65

Temperature(°C)

Date

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S2

Table S1. Mean temperature measured on horizontal and vertical pipes monitored for hot water and recirculation.

System Riser Floor Duration (d) Hot Water Temp. (°C) Mean ± SD Mean Recirc. Temp. (°C) Mean ± SD

Temp. Loss (°C) Horizontal Loop Vertical Horizontal Vertical Horizontal

A na

2 17 58.7 ± 0.09 55.8 ± 0.21 53 ± 0.50 55.9 ± 0.36 0 3 30 57.1 ± 0.28 57.4 ± 0.12 48.8 ± 0.55 54.8 ± 0.14 2.6 4 17 58.4 ± 0.08 58.7 ± 0.09 50.7 ± 0.78 52.4 ± 1.06 6.3 5 17 58.5 ± 0.09 58.6 ± 0.09 48.6 ± 0.91 52.5 ± 1.81 6.1 6 17 58.8 ± 0.08 56.3 ± 0.08 47.7 ± 0.99 50.9 ± 0.79 5.4 7 17 58.3 ± 0.09 58.2 ± 0.26 48.2 ± 1.34 39.8 ± 4.12 18.4 8 17 58.9 ± 0.09 58.9 ±0.10 52.0 ± 0.97 44.9 ± 1.02 14.0 9 17 59.0 ± 0.11 58.4 ± 0.15 53.0 ± 0.55 53.1 ± 0.54 5.3 10 17 59.2 ± 0.18 58.5 ± 0.57 58.3 ± 0.16 50.1 ± 0.57 8.4

B

12N 2 24 54.8 ± 2.56 57.4 ± 2.71 54.2 ± 2.46 51.9 ± 2.55 5.5 3 24 55.1 ± 2.58 58.5 ± 2.87 54.7 ± 2.58 55.5 ± 2.60 3 11R 2 24 58.3 ± 2.76 57.6 ± 2.72 49.5 ± 2.05 53.4 ± 2.42 4.2

3 24 - 56.2 ± 2.65 - 53.5 ± 2.47 2.7

11J

3 24 57.5± 2.77 57.3 ± 2.75 53.6 ± 2.45 55.8 ± 2.60 1.5 4 24 57.9 ± 2.78 58.0 ± 2.79 50.3 ± 2.51 55.5 ± 2.56 2.5 5 24 - 58.1 ± 2.80 - 55.3 ± 2.56 2.8 6 24 - 57.9 ± 2.72 - 54.4 ± 2.64 3.5 4P

2 24 - 56.5 ± 2.70 - 53.2 ± 2.41 3.3 5 24 55.5 ± 2.58 57.2 ± 2.67 51.4 ± 2.27 51.1 ± 2.69 6.1 6 24 55.4 ± 2.60 56.8 ± 2.67 43.1 ± 1.59 52.5 ± 2.34 4.3 SS & 1 3 56.9 ± 0.96 56.5 ± 1.38 53.6 ± 1.53 44.1 ± 1.04 12.4

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S3

Table S2. Faucet characterization and sampling plan.

System Riser Floor ID Type of Faucet Connection Material Mitigated Sampling

T° Profiles Cu-Cold Water Cu-Hot Water Lp

A

10 A Manual Flexible piping x x x

10 B Manual Flexible piping x x x x

9 C Foot-operated Copper x x x x

8 D Manual Flexible piping x x x x

7 E Manual Copper x x x x

7 F Manual Flexible piping x x x x

n.a. 7 G Manual Copper x x x

6 H Electronic Flexible piping x x x x

5 I Electronic Flexible piping x x x x x

5 J Manual Flexible piping x x x x

4 K Electronic Flexible piping x x x

3 L Electronic Flexible piping x x x x

1 M Manual Flexible piping x x

B

6 4P N Electronic Flexible piping x x x

6 11J O Manual Flexible piping x

5 11J P Foot operated Flexible piping x x

4 11J Q Manual Copper x x x x

4 4P R Manual Flexible piping x x x

4 4P S Manual Flexible piping x x x

3 11R T Manual - x x

2 11R U Manual Flexible piping x x x

2 12N V Manual Flexible piping x x x

1 - W Manual Copper x x x

SS - X Manual Flexible piping x x x

Figure

Figure S1. Temperature measured at the water heater outlet (blue) and on the return loop prior to the  water heater (orange) for systems A and B
Table S1. Mean temperature measured on horizontal and vertical pipes monitored for hot water and recirculation
Table S2. Faucet characterization and sampling plan.

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