Dried whey protein fractal aggregates for substituting texturizing additives in dairy products

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HAL Id: hal-02634107

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Dried whey protein fractal aggregates for substituting texturizing additives in dairy products

Domitille de Guibert, Martin François, Anna Kharlamova, Ali Kerjouh, Cécile Le Floch-Fouéré, Guillaume Delaplace, Romain Jeantet

To cite this version:

Domitille de Guibert, Martin François, Anna Kharlamova, Ali Kerjouh, Cécile Le Floch-Fouéré, et al..

Dried whey protein fractal aggregates for substituting texturizing additives in dairy products. 15th International Hydrocolloids Conference, Mar 2020, Melbourne, Australia. �hal-02634107�

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Dried whey protein fractal aggregates for substituting texturizing additives in dairy products

D de Guibert¹, F Martin¹, A Kharlamova², A Kerjouh¹, C Le Floch Fouéré¹, G Delaplace³, R Jeantet¹

1 STLO, INRA, AGROCAMPUS OUEST, 35042 Rennes, France

2 IMMM, UMR-CNRS, 72085 Le Mans, France

3 UMET, INRA, PIHM Team, 59651 Villeneuve d'Ascq, France

*E-mail address: domitille.deguibert@inra.fr

PROFIL

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Science &

Technology of Milk &

Egg

Rennes

A multidisciplinary and multiscale approach, reinforced by two high-calibre facilities:

Dairy Platform Biological Resource Centre

q Structuration / destructuration mechanisms of food matrix:

from structural characterisation to digestion

q Dairy processing and cheese making:

toward sustainable dairy systems

q Microbial interaction:

food matrix and host cell

Please visit http://www6.rennes.inra.fr/stlo_eng

83 standing fellow workers 25 PhD students

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C

ONTEXT

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International Hydrocolloids Conference 2020 / Domitille de Guibert (INRAE) 4 March 2020

Background & RQ Strategy Results / discussion Conclusion

Industrial expectation

• Target regular products properties by using additives

• Use less additives as possible (Clean label)

• Add value to milk protein (technofunctional interest)

PROFIL

2014-2019 Joelle LEONIL

Consumer expectation

• Good organoleptic quality

• More natural and healthy products

Fractal aggregates : Whey protein aggregates

Adapted from Nicolai 2011

WPI, 80°C,

pH 8, NaCl • Repeated pattern

• Soluble

• Low density

activated

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PROPERTIES OF FRACTAL AGGREGATES AND RESEARCH QUESTION

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Increase

viscosity Gel Bind fat droplet in Resist to heating

emulsions Properties of fractal aggregates

/

Limit syneresis

Production at laboratory scale

Incorporation into dairy products

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Produce aggregates at pilot scale and vary their size

2

Stabilize aggregates by concentration and drying

3

Check the functionality

De Guibert D, Martin F, Hennetier M, Gu YY, Le Floch Foueré C, Delaplace G, Jeantet R.

2020. Flow process and heating conditions modulate the characteristics of whey protein aggregates. J Food Eng 264, article 109675, 1-10

Research question

What is the impact of the concentration and drying operations on the cold gelation functionality of fractal aggregates

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Pilot scaleIndustrial scale

100L

400L

Vol : 40L

Vol : 400L

5 kg water.h^-1

100 kg water.h^-1

A

GGREGATES PRODUCTION AND STABILIZATION

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Asymmetrical Flow Field-Flow Fractionation (A4F)

S

IZE CHARACTERIZATION BY A4F

International Hydrocolloids Conference 2020 / Domitille de Guibert (INRAE) 4 March 2020 .06

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S

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.08 Apparent Mw, Rg, Rh

for each population

S

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Asymmetrical Flow Field-Flow Fractionation (A4F)

S

Concentration (mg.mL^-1)

Elution time (min)

Gyration radius (nm)

Non-aggregated proteins

Fractal aggregates

International Hydrocolloids Conference 2020 / Domitille de Guibert (INRAE) 4 March 2020 .06

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C

OLD GELATION PROPERTIES OF FRACTAL AGGREGATES

Cold Gelation

Principle

Reduce electrostatic repulsion to form gel witout the use of heating

Interest

Specific food application (fresh products ; preserve vitamins)

Kharlamova et al (2018)

R = [Ca²⁺]/[Proteins]

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COLD GELATION PROPERTIES OF PILOT & LABORATORY SCALE AGGREGATES

Laboratory scale aggregates

Pilot scale aggregates tan(δ) = ^!_!^""_"

Conclusion :

Pilot aggregates have cold gelation properties similar to those of laboratory

scale aggregates

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COLD GELATION PROPERTIES OF PILOT & INDUSTRIAL SCALE AGGREGATES

Pilot scale aggregates

Industrial scale aggregates

Conclusion :

Industrial aggregates have lower cold gelation properties to those of pilot

aggregates

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S

IZE INCREASE OF THE AGGREGATES AT INDUSTRIAL SCALE

Pilot scale aggregates

Industrial scale aggregates

Conclusion :

The decrease in the functional properties of aggregates produced on an industrial scale

is explained by the increase in their size during production

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Mean residence time

60min 4min

Pilot scale aggregates Industrial scale aggregates

Fraction with high residence time

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CONCLUSION

Research question

What is the impact of the concentration and drying operations on the cold gelation functionality of fractal aggregates

Pilot scale

Industrial scale

Similar cold gelation properties

Lower cold gelation properties

Size increase

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Thank you for your attention

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