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Crystals of fasciculin 2 from green mamba snake venom.
Preparation and preliminary x-ray analysis
Marie Le Du, P. Marchot, Pierre Bougis, Juan-C Fontecilla-Camps
To cite this version:
Marie Le Du, P. Marchot, Pierre Bougis, Juan-C Fontecilla-Camps. Crystals of fasciculin 2 from green mamba snake venom. Preparation and preliminary x-ray analysis. Journal of Biological Chem- istry, American Society for Biochemistry and Molecular Biology, 1989, 264 (35), pp.21401-21402.
�10.1016/S0021-9258(19)30094-8�. �hal-03261830�
THE JOURNAL OF BIOLOGICAL CHEMISTRY
0 1989 by The American Society for Biochemistry and Molecular Biology, Inc. Val. 264, No . 35, Issue of December 15, PP. 21401-21402,1983 Printed in U. S. A.
Crystals of Fasciculin 2 from Green Mamba Snake Venom
PREPARATION AND PRELIMINARY X-RAY ANALYSIS*
(Received for publication, July 20, 1989)
Marie H. le DuS, Pascale Marchot§, Pierre E. Bougisg, and Juan C. Fontecilla-CampsSn
From the jLaboratoire de Cristallographie et Cristallisation des Macromolecules Biologiques, URA 232, Centre National de la Recherche Scientifique and the SLaboratoire de Biochimie, URA 1179, Centre Nationale de la Recherche Scientifique, U 172 Institut National de la Sante et de la Recherche Medicale, Faculte de Medecine, Secteur Nord, Bd Pierre Dramard, 13326 Marseille, Cedes 15, France
Fasciculin 2 from the venom of the green mamba, Dendroaspis angusticeps, has been crystallized. The crystgls are tetrago?al, with unit cell dimensions a = 48.9 A and c = 82.0 A, space group P 41212 or P 43212.
Density measurements and pseudocentering of the hkO zone indicate that there are 16 molecules in the unit cell.
Venoms from the Elapidae family of snakes contain a series of related small proteins (Mr
-
7000) which display a variety of toxic actions. Of these, the best studied are the cardiotoxins and the a-neurotoxins; the three-dimensional structures of one cardiotoxin and several a-neurotoxins have already been determined by x-ray crystallographic methods (1-4). A third group of toxins, called fasciculins because of the muscular fasciculation they provoke when injected in the mouse (5), are known to be very potent inhibitors of several types of acetylcholinesterases (Kt-
10”’ M ) (6). Fasciculins are of considerable interest since they constitute the only known protein inhibitors of these enzymes.Three fasciculins have been characterized to this date:
fasciculins 1 and 2 from Dendroaspis angusticeps venom and toxin C from D. polylepis venom; the reported amino acid sequences of fasciculin 2 and toxin C indicate that fasciculins are structurally related to cardiotoxins and a-neurotoxins (7, 8). In this paper, we communicate the crystallization and preliminary x-ray analysis of fasciculin 2.
EXPERIMENTAL PROCEDURES
The toxin was purified in the following way: lyophilized D. angus- ticeps venom, purchased from Dr. C. Leakey, (Kenya) was fraction- ated by gel filtration chromatography on Sephadex G-50 (Pharmacia LKB Biotechnology Inc.) in 0.1 M ammonium acetate, pH 6.8. The fractions which induced inhibition of rat brain acetylcholinesterase activity were further separated by two successive ion exchange chro- matography steps on CM-Sepharose CL-GB columns (IBF): 1) a linear gradient of 2 X 1 liters of 0.1 to 1 M ammonium acetate, pH 6.8, on a 2.5- X 22-cm column, and 2) a stepwise elution with 0.15 M ammonium acetate, pH 6.8, on a 1 X 200-cm column. The homogeneity of the purified fraction was controlled both by amino acid analysis and by analytical reversed-phase high performance liquid chromatography (9). Acetylcholinesterase activity was determined by the method of Ellman et al. (10) with acetylthiocholine as substrate and crude rat brain synaptosomal fraction (P2) as the enzyme source (11).
* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “aduertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
ll To whom correspondence and reprint requests should be ad- dressed.
RESULTS AND DISCUSSION
Crystals were originally grown by the hanging drop method (12) at 20 “C using tissue culture plates (Limbro, Catalogue No. FB-16-24-7C). Two p1 of a 1-ml reservoir solution con- taining 35% saturation ammonium sulfate and 5 mM P-octyl glucoside buffered with 0.1 M MES,’ pH 6.0, were mixed with the same volume of a 10 mg/ml protein solution in 0.05 M ammonium acetate. The resulting drop was equilibrated against 1 ml of reservoir solution. Medium sized (0.3 x 0.3 x 0.3 mm’), bipyramidal crystals appeared after 2 to 5 days.
Larger crystals (0.7 X 0.4 X 0.4 mm3) were subsequently obtained using 12-p1 sitting drops deposited on a spot plate and equilibrated against a 20-ml solution in a small plastic box. In this case, the protein and reservoir solutions were buffered at pH 6.5. Attempts to grow crystals in the absence of 8-octyl glucoside under these conditions were completely unsuccessful.
X-ray precession photogr!phs show that th? crystals are tetragonal, with a = 48.9 A and c = 82.0 A. Systematic absences along the principal axes indicate that the space group is P 41212 or P 43212. Calculation of the VM (13) for n = 1 and n. = 2 (where n is the numb?r of molecules in the! asymmetric unit) gives values of 3.64 A3/dalton and 1.82 A3/dalton, re- spectively. Since both values are within the experimentally observed VM range, the calculation of the actual number of molecules per asymmetric unit required the determination of the crystal density. Due to the instability of fasciculin crystals in Ficoll solutions (14), the procedure described by Stout and Jensen (15) was used instead a mixture of p-xylene and carbon tetrachloride was carefully adjusted so that previously dried crystals immersed in it would neither sink nor float; the density value, obtained from the weight of a known volume of this mixture was 1.256 g cme3. Assuming that the cell parameters were not changed by this procedure and that the fasciculin molecule has a partial specific volume of 0.75 g
~ m - ~ , which is typical for proteins in general (13), the calcu- lated mass of protein per asymmetric unit is 15,160 daltons.
Since fasciculin has a calculated molecular mass of 6,735 daltons, the density measurement indicates the presence of two molecules in the asymmetric unit.
Another indication of the number of molecules in the unit cell was obtained through examinati2n of the precession pho- tographs. At low resolution (d > 12 A), the hkO zone appears to be pseudocentered with reflections of the type h + k = 2n
+ 1 being systematically absent (Fig. 1). Since centering implies doubling of the number of equivalent positions, this observation is also consistent with the presence of 16 mole- cules in the unit cell. Furthermore, the pseudocentering im-
The abbreviation used is: MES, 4-morpholineethanesulfonic acid.
21401
This is an Open Access article under the CC BY license.
21402 Crystals of Fasciculin 2 from Green Mamba Venom
t
a*-c b*
FIG. 1. Precession photograph of the hkO zone of a fascicu- lin 2 crystal (precession angle = 3O, crystal-to-film distance
= 100 mm). Reflections satisfying the condition h + k = 2n + 1 are systematically absent at low (d > 12 A) resolution.
poses restrictions on the way the two crystallographically independent molecules are oriented relative to each other.
We have collected a complete native data set to 2.0 A
resolution on our Xentronics-Nicolet-Siemens area detector.
Heavy atom searches are in progress.
Acknowledgments-We thank Prof. 0. Dolly and Dr. G. T. Tibbs for fruitful discussions and Prof. H. Rochat for his continuous en- couragement.
Note Added in Proof-Similar fasciculin crystals have been re- ported very recently by (Basu et al.) (Basu, S. p., Hannick, L. I., and Ward, K. B. (1989) Toxicon 27,832 (abstr.).
REFERENCES
1. Rees, B., Samama, J. P., Thierry, J. C., Gilibert, M., Fischer, J., Schweitz, H., and Moras, D. (1987) Proc. Natl. Acad. Sci.
U. S. A. 84,3132-3136
2. Tsernoglou, D., and Petsko, G. A. (1976) FEBS Lett. 68, 1-4 3. Walkinshaw, M., Saenger, W., and Maelicke, A. (1980) Proc.
4. Love, R. A., and Stroud, R. M. (1986) Protein Eng. 1, 1-37 5. Rodriguez-Ithurralde, D., Silveira, R., Barbeito, L., and Dajas, F.
6. Harvey, A. L., Anderson, A. J., Mbugua, P. M., and Karlsson, E.
7. Viljoen, C. C., and Botes, D. P. (1973) J. Biol. Chem. 248,4915- 8. Joubert, F. J., and Taljaard, N. (1978) S. Afr. J. Chem. 31,107- 9. Bougis, P., Marchot, P. E., and Rochat, H. (1986) Biochemistry 10. Ellman, G. L., Coutney, K. D., Andres Jr., V., and Featherstone, 11. Blaustein, M. P., and Ector, A. C. (1976) Biochim. Biophys. Acta 12. Wlodawer, A. M., and Hodgson, K. 0. (1975) Proc. Natl. Acad.
13. Matthews, B. W. (1968) J. Mol. Biol. 33, 491-497 14. Westbrook, E. M. (1976) J. Mol. Biol. 103,659-664
15. Stout, G. H., and Jensen, L. H. (1968) X-ray Structure Determi- nation-A Practical Guide, pp. 79-80, Macmillan Publishing Co., New York
Natl. Acad. Sci. U. S. A. 77, 2400-2404
(1983) Neurochem. Int. 5,267-274 (1984) J. Toxicol. Toxin Rev. 3,91-137 4919
110
25,7235-7243
R. M. (1961) Biochem. Phnrmacol. 7,M-95 419,295-308
Sci. U. S. A. 72,398-399
