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DNA replication stress triggers rapid DNA replication fork breakage by Artemis and XPF

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Academic year: 2021

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Fig 1. Brief replication stress results in rapid replication fork breakage. (A) Quantification by neutral comet assay of DSBs induced, in response to treatment with various doses of HU for up to 24 hours
Fig 2. Rapid replication fork breakage (RRFB) is independent of MUS81. (A) Western blot of whole cell extracts of RKO cells showing depletion of Mus81 by siMus81 compared to a control, siLUC
Fig 3. ARTEMIS and XPF are involved in RRFB. (A) Quantification by neutral comet assay of DSBs induced in response to treatment with 4 mM HU for 4 hours in control cells (siLUC) and in cells depleted of one of various endonucleases
Fig 4. Regulation of ARTEMIS and XPF dependent RRFB. (A) Purification of fork-associated proteins by native- native-iPOND in response to HU (4mM– 4hours)
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