Characterization of muscle glyceraldehyde-3-phosphate dehydrogenase isoforms from euthermic and induced hibernating Jaculus orientalis

In conclusion, our results reveal the possible existence of two different BDH populations in jerboa liver associated to either light or heavy mitochondria: one would be associated

National Center for Energy Sciences and Nuclear Techniques 18 PUBLICATIONS     165 CITATIONS    .

Tissue distribution of the cytosolic and mitochondrial glycerol-3-phosphate dehydrogenase (cGPDH and mGPDH) activities in jerboa (Jaculus orientalis), a hibernator, shows the

coli DH5a (pClost1) was obtained by partial diges- tion with BamHI and NdeI endonucleases and the re- sulting DNA (approximately 1.5 kb) fragment was ligated into plasmid

Their results revealed that neither C149S nor C149S/C281S mutants aggregated, proposing that the active site cysteine of GAPDH plays an essential role in amyloid binding,

RT-PCR amplification using primers constructed from two highly conserved GAPDH regions pro- duced a single cDNA fragment of the expected size ( approx. 0.5 kb ) comprising

(prehibernating) leads to a decrease of BDH-specific activ- ity in both directions, whereas BDH activity is stimulated by combination of cold and food deprivation (hibernat- ing)

1 GAPDH purification steps from human erythrocytes with traditional procedure Proteins were resolved by SDS-PAGE and stained with Coomassie Brilliant Blue (A) or subjected to