Bioinformatics as a critical prerequisite to transcriptome and proteome studies.

Texte intégral

(1)Bioinformatics as a critical prerequisite to transcriptome and proteome studies. Elisabeth Jamet. To cite this version: Elisabeth Jamet. Bioinformatics as a critical prerequisite to transcriptome and proteome studies.. Journal of Experimental Botany, Oxford University Press (OUP), 2004, 55 (405), pp.1977-9. �10.1093/jxb/erh221�. �hal-00157521�. HAL Id: hal-00157521 https://hal.archives-ouvertes.fr/hal-00157521 Submitted on 26 Jun 2007. HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés..

(2) Published in Journal of Experimental Botany 55 (2004) 1977-1979. PMID: 15258172. Bioinformatics as a critical prerequisite to transcriptome and proteome studies Elisabeth JAMET.

(3)

(4) !" #%$ !& &! #' )(+*,-. /0*1!0 23. #546 (

(5) 6 7 08 9:;<$5=>; +? 9:@ BA0A"C

(6) D E C

(7) F G0 !H1I*KJ0 MLN/ J0 / (

(8) L@POGQ>R93S543TVU5U5 WXOGWE D $Y 5=ZY[%\RU]\ = ^ !_"`XW0W1abc A3D EdOGe>W A W0b fg 8 _#`gW0W1abc AD EhOGe1W A bE ? H1!&g_ji!Hk 7lmnoqp

(9) r ? st p . Abstract. U] (

(10) ? n!!. P(

(11) -*,/+H1.u Jvw #xV /0*(

(12) zy !y{/0*{-*0*,/, 7j /0*+ o

(13) !.&

(14) . |*{J0 7 }#n h / J0 #n(+*~ 80r .Hk !*" 7-%

(15) r0r / n3 d"3 y}/ s/h 80r .!I* " n3I* 7 H/

(16) w/+/0(+."# HK !!*0I*0(qp^/ 1/ , #n [* / HZ w/v* / z(+*

(17) 7 V /vH

(18) /+* / H1 7 r0 !Jv. w/v* p', - !!

(19) ! y~,Z -.5 0 ! R -. o

(20) !*,! Z 7/d 8 & *(

(21) ./0/0(+-%J0 7Z

(22) !/+H1 K w#!q 7/o

(23) /0&J Hkn*# 7 7r0 7 /0*1/ 80r Hk !*" 7- # np.

(24) Introduction. ', :*0 !nI*0(!H1/ *" %/ t !*0! ]o

(25) !.&

(26) . :*KJ+ 7

(27) #t F *,J !JZ HK %0I(

(28)

(29) 0!* 7 * yv ! (

(30) / a# r _ &3:p *0

(31) p*0&Hp *0I}p (

(32) /,o3 -* v!*(

(33) !* v-* 7 np " H )c F Hk

(34) ,x

(35) !!

(36) ! y / -*,! [!*0*,/, 7j /0* ( ! 1 ,!&. !*0(

(37) p^T* /v*" #V 3 / (0/, v!3-*,!Iy t K/ *

(38) n r 7/0*0!g! 7wo

(39) VyK 0 ]!H n!!*0*0*0(k 0 3( !*0/

(40) H1 / r #/ 7 G*# .! *+t r w/v* *0 a$0/ J0 y B!p F E

(41) b0bXO

(42) !HK!J+x B!p F E

(43) b0b0Wc F *

(44) n r 7/0H1 x!*0J r0 / 7 G/vH1 PV J. V V j

(45) < *0J & *0 7/0* / (

(46) !*0 " 7/ v r0 -. -zy J0 7 H1*

(47) !J p1 *+t r 7/vHK V J > 7 y J0 nz(

(48) *

(49) !J s/ /+/{ 0 > 8,r0 #jn/

(50) *B/ r !! &1!/

(51) I. ! /0*+Y/ (

(52) !*

(53) 0 gH q

(54) !. o

(55) !*# q / 0 M

(56) /+w/v(

(57) .!! V w/v*1J+J "jn J p-@ /# 7 G/vH1. r0r0 /

(58) ! , # !yK/+* 0 PJ0 !*# 7 .! 7/0* / Br0 / 7 *+ r / HK !J HKjx r ! /0H1 y{ !

(59) d /+H r r wJ+ n !*,-*0(K/ 5r r J0 H1j *0(

(60) r0 *# 7I*(qp L/

(61) I* / H1 7%!*0!Iy} #% R*0/{H1!J

(62) !#n. n*

(63) ! R 0 ! VyZ/ 0 o}-.

(64) . Zt/ Z *0J/ 0 1!*0*0/ 7 w/v*

(65) r0 /,o

(66) .J0 !JB 0y~J0 7 }#t , "/v*" I* / nzy~&H r0 /o

(67) *( F r !!&!.Iy / r w-*" %H1/

(68) J0 -}/ (

(69) *0&nHB

(70) !"#%$'&()*(+(G*0J-, #./"-$#0gpT* / H1 /0*+ !. 7 !J /. V V j

(71) . (

(72) *,. *,J. 80r0 #n/+*. . aV# . r _ &3:p *0

(73) p *0&Hp*0}p (

(74) /o11 7 }#n "I*

(75) J0 8 p# H1.cp. o

(76) !&

(77) .. 3 *,/vH1.. F. . 21=K. =K$]L T !*,J. . n !*,! 3 B!/

(78) H r !J 7/ #V 7

(79) n

(80) B 8 /0*3*" /0*[ V

(81) k/ (

(82) !*, ,pLN/+* / HK . r0 !J0 7/0*+. . !0 !0

(83) J. !*0J. ov !*# !Iy. !/ 7 !J. n*(. r0 HK y. n !*0 v. aV# . r _ &3:p *0

(84) p *0&Hp*0}p (

(85) /o.;3 n 3c as@ V G*0J (

(86) /, . F E

(87) b0bXOcpkT* / H1 7/0*+ /+* r0 /# 7 * & *0 7/0* r0 /o

(88) J0 !Jh*[t o} !5J0 7 }"n *0! Jv*0( 9Z*0 r0 /, %>, > 0 k/ z(}&* / 0 r0 / r /t !J & *0 7/0* H1 !*# 7/0*0 !J a7 8,r HK !*# 7! / G ! /0*0 -*0*,/, 7j /0*c aV# . r _ &3:p 80r #y}p *0 r0 /, tp / (&c. aV r Z !&. . 4 $. )5 F. aV# . r _ &3:p *0

(89) p *0&Hp*0}p (

(90) /os !*# 2 y}p (

(91) 6!J 7+= !. !/# J

(92) c F. E

(93) b0b C c F. =K$]L T 'T;.

(94) aV# . r _ &3:p

(95) J0/ r np / (.c F XT N;. a# & r _ #.:p z( p / ( 7J# s E(5O gOc *0J xTV@ . aV# . r _ &3:p H r p

(96) /

(97) !0 !HBpH r (qp J0 2 r0 /i& 0! J *}J0 8 p # H1cp '0 r r 1&. r0 /,o

(98) .J0 t/+H1 8 -H r . , / Hkn. !!J0I*0( -*0*,/, 7j /0*+[Z (

(99) J / r 7 zov 0r #/ 7 GI* & ,* 7/v*P , BH1 yu! n xHk 70

(100) # ! 70 * 7 y J0 nz(+* / *<J

(101) 7 *# 7 r0 7 w/v*}pY 8 !H r . k1.:

(102) xHK-*0yP 70

(103) * /vH. 5 $'&()#(+(u *0J. /+H r

(104) .n

(105) #J. r r '/ J0 7 v#n !1#X91*0 r0 /, F =K$^L T F 'T; F 5T N; *,J TV@ p. Proteins rich in particular amino acids. $ -+Z!XV V 0 r0 /, 7 * r0 /,o

(106) J0 *# 7 , *(B 8 !H r . ,'/ qr /

(107) / yK!*0*0/ 7j /v*ZJ / , - t -*,! #> , 1 !m* r . !H1&*0/ !!J+p]' ! d!&"n #K/ V

(108) ! r0 /# 7 *+%,o} R

(109) -*Z-. " zyKJ0 *

(110) !Jq_ 8 7 G* nI* ]!0 " 7 z2- !JZ 0y>

(111) r0 #n !*0 :/ * HK / n? @ / aV* WcRH1/ 3n r 7 !Jk 0y~'y n?F U^y} ?F V F *,J 41! ? .! (

(112) /+*

(113) a>. !&n2" K} *0J>U]!H r / F OGe0e C c 0y J / 8 y r0 /+*

(114) 2&@ /0I*

(115) ? ;.! r0 /# 7 *+%a . @ ;@%c%!, ! 7 )2" !JZ 0y F 0(+ !/v*" 7 *# *@ / *0J@ / ? @ / ??

(116) ? U5y 1H1/ F 0 >.

(117) 7q4>! F. 'y nF F / . r #/ 7 * %aR;R@%7c% 0 ! 7 z2" JZ 0yK0(+1/v*" 7 -*" g&* a 0/1! 7 n F GO e0e0W"c ,-*,J : zy

(118) #*

(119) ? ;.! 0 :zy~a.

(120) r /hQ0b cY/ (

(121) G*0z2" !J * r ! n / 0 1a:zy ? cH1/ F 10 . 7 :y. F 3& F / . a 0/1! 7 nF OGe0eWcpq= H1 / r0 /# 7 *+ r0 !J0. 7 !J[ /m,o

(122) d[n(+*,! r r J

(123) 0yx@ \R;N aV# . r _ r n/ p *z 0 qp !p i r / HBp " H1c. ^ (

(124) @. *0J. aV# . r _ &3:p pJ# p Jn o

(125) .! # s^ (

(126) @ c *,J n

(127) /,*( /v*0y{n0/ s ! 0 "/ @ / / :zy. 0 o

(128) } ! !*d /0*(

(129) zy !*0*0/ 7 7 !Jx"1 8 7 *+n* ? &} F @ ;@u/ R ;@p%50&1k*,/# 7

(130) zy[

(131) . !#t / R E0(

(132) W0W0Q0e0bxa O C p D. @ /c F R A (0E D b0Q0baVE0W p A. @ /0c1 *0J[R C (0E

(133) W0b0bxaGOGW p D. @ /0c **,/, 7j 7 !J# 8 7 G*+n* / @q;@%I*k , :91*0 r0 /# F =k$^L T F 'T;{ *,J 5T R;{J0 7 v#n p R C (

(134) W C W0b0bda O C p Q 3 wyc F R C (

(135) W0W0e0W0baGO C p D. Iy,c3-*,JR E0(5O. A W C bda OGQ p D. :zy0c3. . r0 #t *# 7zyB!*0*,/, 7j 7 !J1# R ;@^%*> 0 =k$^L T F 'T; !*0J1'T N; J0 7 }#t F # r 7 zo} / .

(136) *

(137) *0/>* r0 / 7 *+ I*k , :91*0 r0 /# ] *0J TV@ J0 7 }#t #p

(138) \R 0 8 !H r . # r0 /o

(139) J

(140) !J

(141) y ! *# *

(142) n r 7/0H1 J,y /0* r !!m 0y -*}/# & . 4 $%)53aVE

(143) b0b0Wcp$/v*" z(<bXOGb|n,/,K. 0/+HZ/+/0(vy3 7/: 0

(144) D+A b D EK!/# & s/v* w } 5r0 /# 7 * D a/00* F OGe0e D cp *0! F 0 r0 / 7 *10#]/0*0Iy /v*, 70/ 7? zydH1/ F ! *0*,/, %

(145) K!jn & Jh!H1/+*(V V j

(146) r0 /# 7 * ": (0(

(147) , 7 !J 0y , B 0/ p T* , t-HK Z y F !/0*# 7z(POE A n0/K0/+HZ/+/0(vyB 7/. *! * $'&()*(+(. =Z@%OGQ DC0C baVR GO( D E A OGbcjpg50 r0 Hk y t !*,! / , G*0!/

(148) J0 !J r0 /# 7 *[,>/0*0zy /v*

(149) ? @ /[as ,/ 7. . F U%y F >n* F 5 nF c3J0/0H1!* , 3G(

(150) -**0/ :

(151) V &!. !*# /. !jn y !H1/v*(| V j

(152) r0 /# 7 GI* Z#1HK !*# 7/v*, "J* , xT@ J0 7

(153) #t p^3 !Iy F w !/0H r0 st >k@qfg. J0/0H1!I* as@qfgb0bE

(154) W C cJ0 *+*0( r0 /# 7 !#t *00z } 7/ n !J / (

(155) 2.U] @. !HK&zyat" r _ np ? nz qp !3G(+ ?

(156) *&@qf $M = c]!. # zyI*

(157) J0.! 7 !Jk*> 0 =K$YL T F 5TV; !*0J 'T N; J0 7

(158) #t ,p. Proteins having several biological activities. :* 8 !H r . r0 /,o

(159) J0 J 0y r0 /# 7 GI* !H .Iy *,!/+J0*( r 7 zo

(160) > r r0 /# 7 #n #p T r r wJ+#t B r . !/0H 0r s t 3

(161) / ^ 0 VydHZ -H> } >n, *(h 0 kTV@q;Nb

(162) b0e0b0b0QJ0/0H1!* / aV# . r _ &3:p

(163) p !p T7*# 7 @ / !G*3cp / s / , -H. r0 Jv. 7 !J /

(164) n ! / y. r0 /# 7 *+1 0yx@ \N;N !*0J % (

(165) @%p5fg/

(166) / , !H aVR GO(

(167) b0e0Q A b F R A (

(168) b0Q0b0W0b F R 7W( A"C0C b0b *0JdR 7W( D O "

(169) E

(170) bcR1 kJ0 !*# 7 . "J* r0 /# 7 G/0H .hV Jv 3/0* asLN/ J0 . #. 4 $ )5. F E

(171) b0b0W 0 LN/ J0 . 4 $ )5. 5 $'&()*(+(. r0 HK yd! !.'Z#. F} * r

(172) sn

(173) "J " ncp/ %/ 0 !H r0 #t !*# 7Iy. *0*0/ 7j 7 !J #d!0/ / r V * -. !/

(174) J / * !. !/+J1=1

(175) *0J0*0( ?V !. 7 !J r0 /# 7 GI*

(176) d*

(177) =k$^L T !*,J TV@ J0 7 v#n pd3 !Iy F 0 t r

(178) /# 7 *+{ 0/+HZ/v/0( 7/ /

(179) !!/. 0/ / r "V * !& /+J 1 =K ?

(180) *

(181) J0*0( r0 /, 7 * , K#>n,/,Z* 7/,o} B r0 /# 7 !#n B zo

(182) 7y as

(183) 0

(184) !H . 4 $)#5. F E

(185) b0b0bcp050 >G*0*0/ 7 /0*B/ . 5 $ &()#(+(. r0 /# 7 * * , R=B$^L T!*0J TV@ . J0 7 v#n X r0r ! X 7/>

(186) Hkn. !!J0*0(qp\:*3 0 3!/v*" y F !&0 0 n r0 / 7 *+] !/ 7 ! y.

(187) *0*0/ 7j 7 !J[#> rr0 /, 7 #n #*91*0 r0 / 3#Z !IY,1*d 0. 5 $ &()#(+(. 5T N; *0Jx5T;. J0 7 v#n p. Proteins containing several functional domains. @ /# 7 * !/0*# 7GI*0*0( n o} ! &

(188) * w/v*

(189) !KJ0/+H1!* [HK y } P 0r /

(190) . -H 1, !* # n/ n !*,! k!/

(191) H r n/0*h/ R& *0 7/0*0!%J0/+H1!*[t ! !d *0/# M! & &yd*# 7 7r0 7 !J p

(192) V 8 !H r & [k , r0 / 7 * -*,!/

(193) J0 !J 0y<R 7W(0E0E

(194) b D b pTV Z& r0 # !*# zy !*0*0/# 7 7 JP# !! r s/ r0 /# 7 *

(195) *

(196) #n !. 7 !Jx* , k=B$^L T F 'TV; !*0Jx%T N; J0 7

(197) #t ,> v !" n h /v*" 7-* K r0 / I. *,!Hk J Z 1 /vHK!* / 9Z*

(198) *0/>* f *0 7/0* 3 1 9>fE D as@qfgbXO D+A Qc !&&y. @qfXR. #jt/+! 7 !JZ1 > 0 r0 /# 7 *-

(199) *

(200) #n >J0/

(201) H1!I*1@XfgR as@qfgb0b0b D ec%*0/ r0 "n *" %I*1 0 r0 /, 7 *}p ', r0 /# 7 GI*

(202) # 0 / 1*0/ r0 !J 7

(203) . & *

(204) w/v*d ^ , H1/+HZ -*" p n !/v*,Jh 8 !H r . kV 0 / [r0 / 7 *+P v !/+*(

(205) *0( 7/ !HK&Iy / aVR GO(

(206) Q. A b F R GO(

(207) Q.

(208)

(209).

(210)

(211).

(212) I* ? {aHk!*0*,/t ?

(213) *

(214) Jv*0(0cP. ! * . D b *0J[R GO(5O D e0b0bcpgTs 3&1HK !*# 7/0*0 !J[*d , =B$^L T F 5TV; F 5T R;. *0JhxTV@ J0 7 v,t # 0 , y n0//, n&HK&. yh 7/[ s' r0 /# 7 *

(215) *

(216) ,t / ".<aT<_>E. . A e

(217) b D Qcp150&Pn.Hk&. y J0/

(218) #x 8 V h 0. 4 .

(219) * ? &

(220) aHK!*0*0/ n ?.

(221) *

(222) Jv*0(0c%& *1J0/

(223) H1!I*as@qfgbXO C A Wc F g*0/ >

(224) r0 /# 7 *

(225) I*

(226) "n 3J0/+H1!*ka@Xfgb0b0b D ec%/ 0 5. . ".. r0 /# 7 !* !*# *HK-*y~HK !HZ } / , . " * !H1&yqp !HK !HK h V . / R GO( A W0b0Q0b 0 -*0*,/, 7j 7 !J# r0 /# 7 * + *

(227) -w 7 !J p550 h !*,!/+J0 J r0 /# 7 *01 . ( HK >. " *k } 7>J0/0H1!I*a@ f b0bXOGW0ec!*0JB*0/ r0 /# !*-

(228) *

(229) ,t >J0/+H1!* p}', 1-*0*,/, 7j /0*B/ (

(230) *0 R GO(

(231) Q.

(232)

(233). A b[ *,J[R GO( A W0b0Q0b[H . !J , ,/ >/ r0 / 7 /0H1.V J#y J0st jn*+(. 0 r0 #t !*0! 1/ %r 7 o} r0 /# 7 *

(234) *

(235) ,t # I*~" !IX - r0 r /0*+ a7$M0zo}n =kJ0H> }. 4 $ )#5. 4 $ )#5. F E

(236) b0bE. F E

(237) b0b0Wcp%50 y !Iy / *0J r 7 wo} d. ! 7I*

(238) >1 7 !/+H r . 7 !zy Jv !*#.

(239) /+/0(

(240) &- *, w/v*

(241) p.

(242) Conclusion. :. , HK !*# 7/v*, !J HKsn. "!J0*(d!*0*0/# 7 /v*

(243) / z(+*

(244) 7 /0H Hk*# 7 r0 7 w/v*/ n -*

(245) " !/0H r n/0* / J/0H1!*[n !0 p

(246) " & 5!*,J .!!X

(247) /+* / HK 71!*0!Iy}n/ N 1 = *0J3s/ 5r0 /# 7 * t !*0! X , / r0r ! X 7/>

(248) !*K n/

(249) 7 !HK !*# q

(250) / Z 7 I*0( *+t r 7/0H1 -*,!Iy / Jn nI*0( # n 0/ H. 0r / 7 G/vH1.-*,!Iy npP^

(251) . &H r / 7 *,! 3/ !/0H r *( # n]/ 7!*

(252) !JZ1 KJ0 !*# g

(253) /+* / HK 7 t/ Z #]s5K !. ,/,1* *[ 0 h !HK !H *,/+* J0 7 v# d # r -.!&y J0 nz(+*

(254) J s/m!/0. ! I*" 7 ( !RHK !H> !*

(255) r0 /# 7 *+Ya# r _ HK !H1*0/+*}p v/, 7G*0 qp *0 ?

(256) /+ !* p J0 2 cRa G#Z!0

(257) . 4 $ )5. F E

(258) b0b0WcpvT %I*" 7 ( 7 ". J0 7 /0H O0O *+ ? Hk !H> !*0 r0 !Jv. w/v* %!*,J 1

(259) n(

(260) *0! r r J0 r0 !Jv. 7/0*+%!*,J &. V 7 " 0 [ y r m/ J0n r -*,!& B 0 BH1 y v m/ t o

(261) !J| v Z ! *P 0 # npM / G/,ov nF 0 ! o

(262) !*0 /

(263) /+* / HK 7 r0 Jv. 7/0*+ 7/

(264) /

(265) /v(0."!%J+ 7 0/ J

(266) B!

(267) !0

(268) J~Z0 !*

(269) o

(270) r /nz

(271) & : 7/ r0 o

(272) !*# YH 70

(273) #p

(274) T*0J

(275) ! !J F

(276) w/+/0(+"!5J

(277) 7 r0 /,ov !J 7/B

(278) 1 #n !*" !q 7/H r0 /,o} 0 ! Vy~/ (

(279) !*0/

(280) H1 k!*0*0/ 7j /v*

(281) !! !*# 7zy[n,/,*B 0y[y}V 7 !H1 7 t !*

(282) !I*0(~/ M& & ? . -*( 21N=k a !". 4 $ )#5. F E

(283) b0bE0c F n / /+(

(284) /v* ! !/ J0 : 7I.&*0(~ y a

(285) >!H1!J

(286) . E

(287) b0b0Wc F *0J 0r #/ 7 /0H1&"%a$M,/ J, y. 4 $ )#5. F E

(288) b0bXO F L/ J0 . #. 4 $()#5. 4 $ )#5. F. F E

(289) b0b0Wcp. Acknowledgements. ', > ,/ ]( 7 &

(290) 7/@ / ;N - @ /0*# ? U] 2"" / 7!! !!J0*0(/ 0 >HK!* r p ', .

(291) / s/ y1

(292) r0r / 7 !J: 0y3 0 . . + 4 " $! (). . 3$* 4 . 4 +$* 4 $#+() 4 )* 4 & 4 & 4 4 +$* (

(293) 4. F %/ / n F f *0! p. *0J:

(294) .

(295) References.

(296)

(297) "!#

(298) %$&

(299) '()$+*,-+-./01

(300)

(301) 23&456878:9

(302) ;' !#<-+9=!#?>)+A@BDCE9

(303) %-<FCG,CG

(304) H78 -ICKJL?MN7OP :FQ"SRUTVQ-+NW3-XY Z+6[+MP\>23] E

(305) b0b C p 9*0@ / _ 0 M9*0zo} -@ /# 7 GI* *0/1 !J(}

(306) #t p ) 4 *( 4 4 ( &5^_ F 1~O0O A? 1hO0OGe

(307) p

(308) %Z+N

(309) %:1645<JL*,7`;)<>)Pab 7c78(<6[6d:9-+P eCE&JL+-<1?f'+Z&

(310) A7)4gfCE-+6[

(311) 1

(312) P7 'e;6dh+<1

(313)

(314) %i8j`<9Nk3CEjle'+Z<%7lem0-f7bic>)A@ n1.B] E

(315) b0b0W p+@ /# 7 /0H1.,N/ /

(316) /t !Iy v/ *,J" !^Z-. r0 /, 7 *

(317) >/

(318) .J0/ r nR

(319) !I.!*

(320) ! !I r !*+n/

(321) *d

(322) #_'B ."# *,!Iy pHo ) 4 $ "!& 4 "#`_Lp F W C E5O ? WE p. Y+VW36[q2&`MBZ+ *.r+st23:*,-uFJLlr+

(323) H786[L-vQw\ux>)lt,-+PysJm0l'P z m0 2L r+6{B] E

(324) b0bEqp@ /# 7 /0H1& !*,-y n{/ , !"* $'&()#(+( ! !IxZ-.p o ) 4 $'"!& 4 "#w_^ F OGQ A"C

(325) ? OGQ D+A p Y++Z++

(326) |k}J&23w) $+6~78$m0(

(327) 16ckqQ(CEl(P7c78

(328) % : )J&2&] E

(329) b0bXO"pX 7 0*( r r wJ+ HKjK r ! s/ G*0J(

(330) !*,/+H1& 1=k J0 7 }# "p 0o ` *+ $ 4 &(+()# . F OGQ ?j E0Eqp. JL+-CK;] OGe0e D p V j 0 G1!0 7 z2" 7/0* / (

(331) G*0 ~!/ 7 # r /0*0J0*( 7/ G/# & s/v* z } D 0r /# 7 GI* * *

(332) (

(333) !*0. r !*" n 0y !*# 7n !*+t (} !*0 p () 4 ()( ()#.,^L E

(334) e0Q ? 0W b D p H>;]=k F. D _ !J ! !J. )*(+$. !65)JLlP abNW36H$k}MB)j`N-E"Qj`

(335) %e$&+*CE y-+Z+

(336) NW}MB`/0 :Z*.-+-Kt5 / NWf : `(+ 7O.Rl23P V@ r+

(337) %92&>)] E

(338) b0bEqpqf ? . -*( [HK jt -*(

(339) ;=K n !*

(340) ! ( zy1&H r0 /o

(341) R(

(342) !*0/0HK *0*0/ 7j /v*}pP 4 + 4 ()# .,^ F b0bE

(343) e+pO ? b0bE

(344) e

(345) psOEqp t,: 6O@A6H$&BCKJLY>)*.+

(346) H7.Qj`] OGe0e C pM 8 7 *

(347) n&*_ r 7 zo} mH1/ F>& *0 7/0*0!kn 7 F r / ? G*+n 7/0*0!}!/+J0 " F *0J r y

(348) /0(

(349) !*yqpL & 4 )(+$N( +()+ O A Q ? QEqp CE<

(350) %$&L*.2&t,-3Z<\MB$3-<Kj`B2& 78q/0] E,b0b0b pY@ /# 7 !#t x! zo

(351) y / $'= 1 C O F . 0w/ / r #V ~* !. !/+J1=K ?

(352) *

(353) J0*( r0 /# 7 GI* F n/+. 7 !J /+H o0 ( 4 $$ 4 )p F O A? O

(354)

(355) p.

(356) Ju 7/

(357) !!/ ! !Gp. MBZ+ *.r+q`t,Y+zW36dG2L"!#*. 78-{JCu2L *.-GFJLY2L r<6B] E

(358) b0b0W p^@ /# 7 /0H1. ,!*(} #3I*h , B 8 !! ! . HK 8 /

(359) .J0/ r nV3! !.M r -*

(360) n/+*d

(361) #3I*

(362) J #J 0y *(

(363) ! !.&! 7/ p ! $ 4 w^ F OGb C Q ? OGb A e

(364) p m

(365) %+ 7c7tQCG9P P7c70Q(B] E

(366) b0bXOp#;N n k!*,J U]/+ U*g_-=$]L T%

(367) ( !*0 ? -*" 7 !J #t/

(368) #p ) 4 *( 4 4 ( &5_L F OGW0Q ? O C b

(369) p.

(370) 23$3Ul23+-+:Z+

(371) W3- Z<1

(372) 45

(373) %aca;) /0 6[+

(374) 5t,(N78-+;)Q6d:*,-<CE /

(375) * *.

(376) F'e/ <99 et,+-@ ] _LP^ ] R;[%d=K\M= F k*0/o

(377) !%J0 7 }"n /

(378) .J0/ r ns%*# 7 G( !+H1 !H> !*0 r0 /# 7 * p )*(+$ !&". #() . ^+ F O D0? E D p 23<NP 78

(379) lBCE] OGe0e0W p V j 0 3 *0J *0 7/0*k/ Xr &!*" %! ! 1!& r0 /# 7 * p& & 4 )*(+$ 4 )#) e ? E

(380) W+p j

(381) %:-+P7c78(>)-+:- QY(6[Z+L

(382) K45] E

(383) b0b0W p ! !IK! ? / !*" 7 !JP(

(384) *0/+H1. r0r0 /

(385) ! o

(386) !!K*, !*0J *, 80r ! 7 Jm!/0H r . 8 Vy / , t/ 7 *0*0(P* r !-

(387) "p< +() o 4 4 +$')P $'(+". p F O

(388) "E5O ? O

(389) WE p \*,Z+ 7P ] E

(390) b0b0W p ]H r .!!!*0!y n1/ G* r w/v*,-Y! 7wo

(391) Vy* ,

(392) .J0/ r n (

(393) *,/+H1 p 4 + 4 ^LP_ F

(394) C E ?

(395) C

(396) D p.

(397)