Enzymatic Synthesis of Dithiolopyrrolone Antibiotics Using Cell-Free Extract of Saccharothrix algeriensis NRRL B-24137 and Biochemical Characterization of Two Pyrrothine N-Acyltransferases in This Extract.

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Eprints ID : 10818

To link to this article : DOI : 10.4014/jmb.1309.09035

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To cite this version :

Saker, Safwan and Almousa Almaksour, Ziade

and Chorin, Anne-Claire and Lebrihi, Ahmed and Mathieu, Florence

Enzymatic Synthesis of Dithiolopyrrolone Antibiotics Using

Cell-Free Extract of Saccharothrix algeriensis NRRL B-24137 and

Biochemical Characterization of Two Pyrrothine

N-Acyltransferases in This Extract. (2014) Journal of microbiology

and biotechnology, vol. 24 (n° 1). pp. 26-35. ISSN

1738-8872

Any correspondance concerning this service should be sent to the repository

administrator:

staff-oatao@listes-diff.inp-toulouse.fr

Enzymatic Synthesis of Dithiolopyrrolone Antibiotics Using Cell-Free

Extract of

Saccharothrix algeriensis NRRL B-24137 and Biochemical

Characterization of Two Pyrrothine N-Acyltransferases in This Extract

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Introduction

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Materials and Methods

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The semi-synthetic medium (SSM) [2] containing 15 g/l of D (+)

glucose (Acros) was used. Sa. algeriensis precultures were prepared in 250 ml Erlenmeyer flasks containing 50 ml of culture medium inoculated with spores (2 × 107

CFU/ml). Cultures grown in 500 ml Erlenmeyer flasks containing 100 ml of SSM were inoculated with 5 ml of 48 h aged preculture. The culture was incubated at 30°C at 240 rpm for 72 h on a rotary shaker (New Brunswick Scientific Company, NJ, USA). .0//23"00*45,"-&,*!"06-"-,'#(*7."%$0*45,"-&,8

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Discussion

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Acknowledgments

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