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The tumorigenic diversity of the three PLAG family members is associated with different DNA binding capacities.

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Academic year: 2021

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Figure

Fig. 1. PLAGL1 binds a different consensus sequence to PLAG1 and PLAGL2. EMSAs were performed with recombinant PLAG proteins produced in vitro in reticulocyte lysates
Fig. 2C). This stimulation is completely abolished when the core and cluster are mutated (mCOmCLU)
Fig. 3. The mitogenic stimulation of NIH3T3 cells overexpressing PLAG1 and PLAGL2. Mock-, PLAG1-, and PLAGL2-expressing NIH3T3 cells were grown in DMEM supplemented with either 5% (A) or 1% (B) FCS and counted daily
Fig. 4. Overexpressed PLAG1 and PLAGL2 proteins are able to transform NIH3T3 cells. A, the mock-, PLAG1-, and PLAGL2-expressing NIH3T3 cells were used for focus-forming assay in medium containing 1% FCS or 10%
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