A part of the VOCs are constantly emitted by the strain during their growth
VOCs specifically emitted by the toxigenic strain have been identified. They are potentiel biomarkers to develop
specific sensors
VOCs emitted by toxigenic and non toxigenic strains are
different l.josselin@uliege.be https://be.linkedin.com/in/ laurie-josselin-a0388416b https://www.researchgate.net /profile/Laurie_Josselin
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VOCs emitted by toxigenic,non toxigenic and both strainsCan fungal volatile organic compounds be used to
develop aflatoxin-specific sensors?
Laurie Josselin
1, Marthe De Boevre
2, Caroline De Clerck
1, Moretti Antonio
3, Soyeurt Hélène
1and
Marie-Laure Fauconnier
11 2 3
For the moment, mycotoxins are analyzed by HPLC-MS/MS a long, expensive and descructive method. The purpose of the present
work is to identify volatile organic compounds (VOCs) markers that are produce together with mycotoxins by the fungi in order
to develop specific sensor.
B
C o n ce n tr a ti o n (M )Retention time (min) 1 0.5 10 20 0 C o n ce n tr a ti o n (M )
Retention time (min) 1 0.5 10 20 0 100 50 A b o n d a n ce (% ) m/z 0 100 50 A b o n d a nce (% ) m/z 0 100 50 A b o n d a n ce (% ) m/z 0
C
see CA
Foodstuff (corn, wheat, rice, etc.)
can be contaminated by several
filamentous fungal species (Aspergillus, Fusarium and Penicillium) in pre or
post-harvest conditions.
1
Fungi grow in field or during storage. They produce secondary metabolites named mycotoxins.2
Mycotoxines3
Mycotoxin's contamination can cause severe illnesses upon chronic exposure and can even lead to death after acute exposure.
5
4
Mycotoxins are thermoresistant, even after hot or cold treatment they are still toxic.
Context
Method
Objective
Results
Outline
GHENT UNIVERSITY see CSeparation and analysis of trapped VOCs
C o m m o n (4 9 % ) N o n T ox ig e n ic (3 5 % ) Tox igen ic (16%) SENSOR FUNGI VOCs ASPERGILLUS SP. VOCs 1-octen-3-ol 3-methylbutan-1-ol octan-3-one 2-methylbutanal VOCS specific to toxigenic strain 2-methylbutanal Undecan-2-one 3-methylbutanal
Increase sensitivity of the method
Correlate VOCs emission and mycotoxin production
Consiglio Nazionale delle Ricerche