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Tropical basil effect on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) oviposition response

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Tropical basil effect on Tuta absoluta Meyrick

(Lepidoptera: Gelechiidae) oviposition response

YAROU B. B.

*

, FINGU MABOLA J. C., VERHEGGEN J. F. and FRANCIS F.

Laboratory of functional and evolutionary Entomology – Gembloux Agro-Bio Tech (University of Liège)

boniyarou1981@gmail.com ; entomologie.gembloux@ulg.ac.be)

Problematic

The tomato leafminer, Tuta absoluta is one of the most important pests of tomato in several countries around the world. The larvae can destroy all aerial parts of plants resulting in severe yield losses.

worldwide distribution

EPPO PQR

In this study, we assess the oviposition deterring effect of tropical basil (Ocimum gratissimum) on this tomato leafminer.

Methodology and results

Behavioral assays

Dual-choice behavioral assays performed in flight tunnels (230 × 45 × 45 cm) with plants (a) and EO (b).

PO: Paraffin oil EO

Plant Compounds Thymol Plants (%) - EO (%) 33,3

p-cymene 33,5 20,4 γ-terpinene 23,6 16,9 Myrcene - 3,9 α-terpinene 7,2 α-thujene 6,7 -

a

b

Plants volatiles and essential oil (EO) analyse

These results suggest a valuable potential of topical basil and associated essential oils as a component of integrated management strategies against the tomato leafminer.

Eighteen and 20 constituents were identify respectively in plants volatiles and EO.

Major compound identified for plants VOC and EO

Plants volatiles were collected with the solid-phase micro-extraction method.

Conclusion

Larvae damage on tomato leaves (a) stems (b), fruits (c)

Because synthetic insecticides lead to resistance and have adverse effects on producers’ health and natural enemies, there is a need for alternatives.

Average number of eggs laid by T. absoluta females

on tomato plant after 48h (Mean of 6 replicates). TB: Tropical basil, PO: Paraffin oil

Acknowledgments

22 May 2018 - Ghent, Belgium

Apparatus of GC-MS analyses

1.0 mg EO 0.5 mg EO

Références

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