Biological variations during a race cycling

Biological variations during a race cycling

Biological variations during a race cycling

Fig. 5 Evolution of hsTnT

Background

Background

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_:

The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the

literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released

during an international cycling race.

Materials and Methods

Materials and Methods

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Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3

hours (T3) after an international cycling race of 176 kilometers in Belgium for the determination of cardiac

and metabolic biomarkers:

red blood cells (RBC)

haemoglobin (HgB)

creatinin (Cr)

highly sensitive troponin T (hsTnT)

myoglobin (MYO)

NT-proBNP

All automated assays were performed according to the manufacter’s specifications.

For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used.

Conclusions:

Our results show that stress generated by a cycling race could be the cause for the different metabolic

variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial

tissue. Its increase can then be considered as being of interest.

Acknowledgement: This experimentation was partially financed by “Adeps 2010” grants (Léon Frédéricq Funds).

Results:

Results:

•RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002) (Fig. 1 and

2).

• Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3

p=0.0001)(Fig.3). These changes might be related to renal flow depletion during exercise.

•MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3,

however the T3 level stay higher than T0 (p=0.014) (Fig.4).

•The stress delivered from the physical activity performed during the race induced a significant variation of

hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of

the exercise (T0-T3: p<0.0001) (Fig.5) .

•The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the

same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0

and T1 and between T0 and T3 for NT-proBNP (Fig.6).

•These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about

cellular necrosis or irreversible injury.

Fig.6 Evolution of NT-proBNP

Legend:

These figures represents

the kintetic of the blood

biomarkers (cardiac and

metabolic) released during

an international cycling race

R 1 ; M o y . M o i n d r e s C a r r é s E f f e t c o u r a n t : F ( 2 , 2 6 ) = 2 1 , 0 8 4 , p = , 0 0 0 0 0 D é c o m p o s i t i o n e f f i c a c e d e l ' h y p o t h è s e L e s b a r r e s v e r t i c a l e s r e p r é s e n t e n t l e s i n t e r v a l l e s d e c o n f i a n c e à 0 , 9 5 T 0 T 1 T 3 R 1 - 0 , 0 1 0 , 0 0 0 , 0 1 0 , 0 2 0 , 0 3 0 , 0 4 0 , 0 5 0 , 0 6 0 , 0 7 V D _ 1 R 1 ; M o y . M o i n d r e s C a r r é s E f f e t c o u r a n t : F ( 2 , 2 6 ) = 6 , 9 9 5 9 , p = , 0 0 3 7 1 D é c o m p o s i t i o n e f f i c a c e d e l ' h y p o t h è s e L e s b a r r e s v e r t i c a l e s r e p r é s e n t e n t l e s i n t e r v a l l e s d e c o n f i a n c e à 0 , 9 5 T 0 T 1 T 3 R 1 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 1 0 0 1 1 0 1 2 0 1 3 0 V D _ 1 R 1 ; M o y . M o i n d r e s C a r r é s E f f e t c o u r a n t : F ( 2 , 2 6 ) = 4 0 , 6 9 5 , p = , 0 0 0 0 0 D é c o m p o s i t i o n e f f i c a c e d e l ' h y p o t h è s e L e s b a r r e s v e r t i c a l e s r e p r é s e n t e n t l e s i n t e r v a l l e s d e c o n f i a n c e à 0 , 9 5 T 0 T 1 T 3 R 1 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 1 0 0 1 1 0 1 2 0 1 3 0 1 4 0 V D _ 1

Fig.4 Evolution of Myoglobin

R 1 ; M o y . M o i n d r e s C a r r é s E f f e t c o u r a n t : F ( 2 , 2 6 ) = 7 , 0 7 0 4 , p = , 0 0 3 5 3 D é c o m p o s i t i o n e f f i c a c e d e l 'h y p o t h è s e L e s b a r r e s v e r t i c a l e s r e p r é s e n t e n t l e s i n t e r v a l l e s d e c o n f i a n c e à 0 , 9 5 T O T 1 T 3 R 1 4 , 5 4 , 6 4 , 7 4 , 8 4 , 9 5 , 0 5 , 1 5 , 2 5 , 3 V D _ 1

Fig. 1 Evolution of Red Blood Cells

R 1 ; M o y . M o in d r e s C a r r é s E f f e t c o u r a n t : F ( 2 , 2 6 ) = 7 , 6 4 7 7 , p = , 0 0 2 4 4 D é c o m p o s it io n e f f ic a c e d e l'h y p o t h è s e L e s b a r r e s v e r t ic a le s r e p r é s e n t e n t le s in t e r v a lle s d e c o n f ia n c e à 0 , 9 5 T 0 T 1 T 3 R 1 1 4 , 0 1 4 , 2 1 4 , 4 1 4 , 6 1 4 , 8 1 5 , 0 1 5 , 2 1 5 , 4 1 5 , 6 1 5 , 8 V D _ 1

Fig. 2 Evolution of Haemoglobin

R 1 ; M o y . M o i n d r e s C a r r é s E f f e t c o u r a n t : F ( 2 , 2 6 ) = 2 9 , 2 3 3 , p = , 0 0 0 0 0 D é c o m p o s i t i o n e f f i c a c e d e l 'h y p o t h è s e L e s b a r r e s v e r t i c a l e s r e p r é s e n t e n t l e s i n t e r v a l l e s d e c o n f i a n c e à 0 , 9 5 T 0 T 1 T 3 R 1 7 , 5 8 , 0 8 , 5 9 , 0 9 , 5 1 0 , 0 1 0 , 5 1 1 , 0 1 1 , 5 1 2 , 0 1 2 , 5 1 3 , 0 V D _ 1

Fig. 3 Evolution of Creatinin

Le Goff C.

Le Goff C.

1

1

, Kaux J.-F.

_{, Kaux J.-F.}

3

3

, Goffaux S.

_{, Goffaux S.}

3

3

, Melon P.

_{, Melon P.}

2

2

, Fillet M.

_{, Fillet M.}

4

4

, Chapelle J.-P

_{, Chapelle J.-P}

1

1

.

_.

1

Department of Clinical Chemistry, University Hospital of Liege, Belgium

2

Department of Cardiology, University Hospital of Liege, Belgium

3

Department of Motility Sciences, University of Liege, Belgium