• SpermatocritSpermatocrit
Effects of chronic confinement stress on
sperm quality in Eurasian perch (Perca
fluviatilis)
C
E
F
R
A
T. Tomson
1,3, S. Esnault
1, C. Prignon
1, J.Ponthier
2, S. Deleuze
2, C. Mélard
1and C.Rougeot
11University of Liège, Aquaculture Research and Education Center (CEFRA), Chemin de la Justice, 10,
B-4500 Tihange, Belgium. C.Rougeot@ulg.ac.be; http://www.cefra.ulg.ac.be
2Equine Clini, Veterinary Medecine Faculty, ULg, Bld de Colonster 20, B-4000 Liège, Belgium 3ASBL CERER Pisciculture, Chemin de la Justice, 10, B-4500 Tihange, Belgium
Introduction
Introduction
Poster presented at 2nd FVM ScientificMeeting October 19, 2012
Liège (Belgium)
Materials and methods
Materials and methods
Results and discussion
Results and discussion
Conclusions
Conclusions
References
References
Rougeot, C., Nicayenzi, F., Mandiki, S.N.M, Rurangwa, E., Kestemont, P., Mélard, C. 2004. Comparative study of the reproductive characteristics of XY male and hormonally sex-reversed XX male Eurasian perch, Perca fluviatilis. Theriogenology 62 : 790-800.
Schreck, C.B. 2010. Stress and fish reproduction : the role of allostasis and hormesis. General and Comparative Endocrinology 165, 549-556.
Schreck, C.B., Contreras-Sanchez, W., Fitzpatrick, M.S. 2001. Effects of stress on fish reproduction, gamete quality, and progeny. Aquaculture 197, 3-24. Ackowledgements: study supported by FRS - FNRS (FRFC n°2.4570.06) and SPW Emploi
• Sperm concentration, motily and velocity did not change during the final maturation period of males breeders Sperm concentration, motily and velocity did not change during the final maturation period of males breeders except for the spermatocritexcept for the spermatocritwhere where isolated males showed lower values whatever the sampling time (p<0,05)
isolated males showed lower values whatever the sampling time (p<0,05)
• Sperm quality, mainly motility and velocity, was not significantly modified by confinement stress.Sperm quality, mainly motility and velocity, was not significantly modified by confinement stress.
Sperm concentration and motility
Sperm concentration and motility
Sperm collection and CASA measurement
Sperm collection and CASA measurement
• 3 males per batches3 males per batches • CASA measurement : CASA measurement :
• Motile sperm (%) Motile sperm (%) • Progressive sperm (%)Progressive sperm (%) • Velocity:Velocity:
• VAPVAP • VCLVCL • VSLVSL • Sperm concentrationSperm concentration
• Counted with BCounted with Büükerker’’s cells cell • SpermatocritSpermatocrit
Sperm velocity
Sperm velocity
• VAP, VCL and VSL are closed to VAP, VCL and VSL are closed to those obtained in a previous those obtained in a previous study (Rougeot et al., 2004). study (Rougeot et al., 2004). • Confinement Confinement did did not not
significantly (p>0.05) changed significantly (p>0.05) changed the sperm velocity parameters the sperm velocity parameters in perch breeders during the in perch breeders during the final maturational period. final maturational period. • Velocity parameters were not Velocity parameters were not
different between the two different between the two sampling times
sampling times • Mature breeders > 2 years oldMature breeders > 2 years old
• 3 confinement levels in duplicate: 3 confinement levels in duplicate: • Natural chilling process (from 4°C to Natural chilling process (from 4°C to
15°C) in Recirculating Aquaculture 15°C) in Recirculating Aquaculture System
System
• 2 dates of prelevement: early March 2 dates of prelevement: early March and last March
and last March
Experimental design
Experimental design
Perch,
Perch, Perca fluviatilisPerca fluviatilis seems highly sensitive to aquacultural stressors (Acerete et al., 2004; Jentoft et al., 2005). In fish, stress seems highly sensitive to aquacultural stressors (Acerete et al., 2004; Jentoft et al., 2005). In fish, stress affects all life functions and particularly reproduction and gamete quality (Sheck, 2001; 2010). The aim of this study is to assess affects all life functions and particularly reproduction and gamete quality (Sheck, 2001; 2010). The aim of this study is to assess if chronic confinement, encountered in intensive rearing system, may modify the sperm qualityduring the reproductive period. if chronic confinement, encountered in intensive rearing system, may modify the sperm qualityduring the reproductive period.
• Sperm concentration ranged from 61.5 to 70.5 Sperm concentration ranged from 61.5 to 70.5 x10
x1099cells/ml and were not significantly different between cells/ml and were not significantly different between
the confinement level (p>0.05) the confinement level (p>0.05)
• The MOT (%) and progressive MOT (%) significantly The MOT (%) and progressive MOT (%) significantly (p>0.05) decreased with time after spermatozoa (p>0.05) decreased with time after spermatozoa activation
activation
• Sperm concentration and motilty (%) were not different Sperm concentration and motilty (%) were not different between the two sampling times
between the two sampling times
0 10 20 30 40 50 60 V A P ( µ m /s ) 15s 30s 45s isolé 4m 1m 40 50 60 70 80 90 100 110 120 V C L ( µ m /s ) 15s 30s 45s isolé 4m 1m 0 5 10 15 20 25 30 35 40 V S L ( µ m /s ) 15s 30s 45s isolé 4m 1m 40 50 60 70 80 90 100 110 120 V C L ( µ m /s ) 15s 30s 45s isolé 4m 1m T1 T1 T2T2 12♂ 12♂/12♀/12♀ 12♂/12♀12♂/12♀ 12♂/12♀ 12♂/12♀ 3♂/3♀ 3♂/3♀ 3♂/3♀3♂/3♀ 1♂ 1♂ 1♂1♂ 1♂1♂
T1
T1
T2
T2
1600l
1600l
500l
500l
144l
144l
Comparative sperm VAP, VSL and VCL of perch breeders reared at 3 confinement levels during the
Comparative sperm VAP, VSL and VCL of perch breeders reared at 3 confinement levels during the
reproductive period reproductive period 12♂/12♀ 12♂/12♀ 12♂/12♀12♂/12♀ 3♂/3♀ 3♂/3♀ 3♂/3♀3♂/3♀ 1♂ 1♂ 1♂1♂ 1♂1♂