No programa preventivo, o treinamento físico aeróbico, de intensidade moderada:
¾ Não alterou o tamanho da placa aterosclerótica, mas reduziu o conteúdo de gordura e aumentou o conteúdo de colágeno das placas;
¾ Evitou o ganho de massa corporal, diminuiu a pressão arterial caudal, evitou o aumento do colesterol total, mas não modificou as frações;
¾ Não provocou alterações nas citocinas plasmáticas TNF-α e IL- 6 e na atividade da MMP-9 plasmática;
¾ Diminuiu a expressão de CD40 na placa aterosclerótica, mas não alterou a expressão de CD40L, a expressão da MMP-9 e a formação da nitrotirosina nas placas.
No programa terapêutico, o treinamento físico aeróbico, de intensidade moderada:
¾ Não alterou o tamanho e o conteúdo de gordura da placa aterosclerótica, mas aumentou o conteúdo de colágeno da placa;
¾ Diminuiu a pressão arterial caudal, mas não provocou a perda de massa corporal, não diminuiu o colesterol total plasmático e não modificou as frações;
¾ Não provocou alterações nas citocinas plasmáticas TNF-α e IL-6 e na atividade da MMP-9 plasmática;
¾ Não alterou a expressão da MMP-9 e a formação da nitrotirosina nas placas ateroscleróticas e devido ao reduzido numero de casos, não foi possível tirar conclusões a respeito da expressão da via de sinalização CD40/CD40L na placa aterosclerótica.
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Anexo 2: Composição da dieta aterogênica
COMPONENTE QUANTIDADE
CARBOIDRATOS 51,56%
PROTEÍNAS (CASEÍNA COMERCIAL) 17%
LIPÍDIOS 18%: Gordura de coco – 12% Óleo de soja – 4,75% Colesterol – 1,25% FIBRAS 5% MISTURA DE MINERAIS 3,5% MISTURA DE VITAMINAS 1%
Anexo 3: Protocolo de treinamento físico em esteira rolante para camundongos
Frequência Duração Velocidade 15 m/min
(dias) (min) Aquecimento Corrida Recuperação
SEMANA 1
1 30 5m/min (10) 10m/min (10) 8m/min (10)
2 30 5m/min (5) 8m/min (5) 12m/min (10) 8m/min (5) 5m/min (5)
3 35 5m/min (5) 8m/min (5) 12m/min (15) 8m/min (5) 5m/min (5)
4 35 6m/min (5) 8m/min (5) 12m/min (15) 8m/min (5) 5m/min (5)
5 40 7m/min (5) 10m/min (5) 15m/min (20) 10m/min (5) 5m/min (5)
SEMANA 2
1 40 7m/min (5) 10m/min (5) 15m/min (20) 10m/min (5) 7m/min (5)
2 40 8m/min (5) 12m/min (5) 15m/min (20) 12m/min (5) 8m/min (5)
3 45 8m/min (5) 12m/min (5) 15m/min (25) 12m/min (5) 8m/min (5)
4 45 8m/min (5) 12m/min (5) 15m/min (25) 12m/min (5) 8m/min (5)
5 50 8m/min (5) 12m/min (5) 15m/min (30) 12m/min (5) 8m/min (5)
SEMANA 3
1 50 8m/min (5) 12m/min (5) 15m/min (30) 12m/min (5) 8m/min (5)
2 55 8m/min (5) 12m/min (5) 15m/min (35) 12m/min (5) 8m/min (5)
3 60 8m/min(5') 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
4 60 8m/min (5) 12m/min(5) 15m/min (40) 12m/min (5) 8m/min (5)
5 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
SEMANA 4
1 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
2 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
3 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
4 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
5 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
SEMANA 5
1 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
2 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
3 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
4 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
5 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
SEMANA 6
1 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
2 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
3 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
4 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)
5 60 8m/min (5) 12m/min (5) 15m/min (40) 12m/min (5) 8m/min (5)