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QUANTIFICATION AND STATISTICAL ANALYSIS

Quantitative analysis (cell counting, c-fos expression and axon length) was performed in a double-blind manner using Fiji or Adobe Photoshop and a superimposed grid feature. Statistical analysis was performed using GraphPad Prism 7. P<0.05 was considered as statistically significant (Tables S1-S6).

REFERENCES

Althammer, F., and Grinevich, V. (2017). Diversity of oxytocin neurons: beyond magno- and parvocellular cell types? J Neuroendocrinol.

Althammer, F., Jirikowski, G., and Grinevich, V. (2018). The oxytocin system of mice and men - Similarities and discrepancies of oxytocinergic modulation in rodents and primates. Peptides.

Cetin, A., Komai, S., Eliava, M., Seeburg, P.H., and Osten, P. (2006). Stereotaxic gene delivery in the rodent brain. Nat Protoc 1, 3166-3173.

Ciocchi, S., Herry, C., Grenier, F., Wolff, S.B., Letzkus, J.J., Vlachos, I., Ehrlich, I., Sprengel, R., Deisseroth, K., Stadler, M.B., et al. (2010). Encoding of conditioned fear in central amygdala inhibitory circuits. Nature 468, 277-282.

Dogbevia, G.K., Marticorena-Alvarez, R., Bausen, M., Sprengel, R., and Hasan, M.T. (2015). Inducible and combinatorial gene manipulation in mouse brain. Front Cell Neurosci 9, 142.

Dogbevia, G.K., Robetamanith, M., Sprengel, R., and Hasan, M.T. (2016). Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain. Mol Ther Nucleic Acids 5, e309.

Ehrlich, I., Humeau, Y., Grenier, F., Ciocchi, S., Herry, C., and Luthi, A. (2009). Amygdala inhibitory circuits and the control of fear memory. Neuron 62, 757-771.

Eliava, M., Melchior, M., Knobloch-Bollmann, H.S., Wahis, J., da Silva Gouveia, M., Tang, Y., Ciobanu, A.C., Triana del Rio, R., Roth, L.C., Althammer, F., et al. (2016). A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.

Neuron 89, 1291-1304.

Fadok, J.P., Krabbe, S., Markovic, M., Courtin, J., Xu, C., Massi, L., Botta, P., Bylund, K., Muller, C., Kovacevic, A., et al. (2017). A competitive inhibitory circuit for selection of active and passive fear responses. Nature 542, 96-100.

Frijling, J.L., van Zuiden, M., Koch, S.B., Nawijn, L., Goslings, J.C., Luitse, J.S., Biesheuvel, T.H., Honig, A., Bakker, F.C., Denys, D., et al. (2014). Efficacy of oxytocin administration early after psychotrauma in preventing the development of PTSD: study protocol of a randomized controlled trial. BMC Psychiatry 14, 92.

Gomez, J.L., Bonaventura, J., Lesniak, W., Mathews, W.B., Sysa-Shah, P., Rodriguez, L.A., Ellis, R.J., Richie, C.T., Harvey, B.K., Dannals, R.F., et al. (2017). Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Science 357, 503-507.

Grund, T., Goyon, S., Li, Y., Eliava, M., Liu, H., Charlet, A., Grinevich, V., and Neumann, I.D. (2017).

Neuropeptide S Activates Paraventricular Oxytocin Neurons to Induce Anxiolysis. J Neurosci 37, 12214-12225.

Guenthner, C.J., Miyamichi, K., Yang, H.H., Heller, H.C., and Luo, L. (2013). Permanent genetic access to transiently active neurons via TRAP: targeted recombination in active populations. Neuron 78, 773-784.

Hamamura, M., Leng, G., Emson, P.C., and Kiyama, H. (1991). Electrical activation and c-fos mRNA expression in rat neurosecretory neurones after systemic administration of cholecystokinin. J Physiol 444, 51-63.

Heidbreder, C.A., and Groenewegen, H.J. (2003). The medial prefrontal cortex in the rat: evidence for a dorso-ventral distinction based upon functional and anatomical characteristics. Neurosci Biobehav Rev 27, 555-579.

Herry, C., Ferraguti, F., Singewald, N., Letzkus, J.J., Ehrlich, I., and Luthi, A. (2010). Neuronal circuits of fear extinction. Eur J Neurosci 31, 599-612.

Katoh, A., Fujihara, H., Ohbuchi, T., Onaka, T., Young, W.S., 3rd, Dayanithi, G., Yamasaki, Y., Kawata, M., Suzuki, H., Otsubo, H., et al. (2010). Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary. J Endocrinol 204, 275-285.

Kessler, R.C., and Bromet, E.J. (2013). The epidemiology of depression across cultures. Annu Rev Public Health 34, 119-138.

Kitamura, T., Ogawa, S.K., Roy, D.S., Okuyama, T., Morrissey, M.D., Smith, L.M., Redondo, R.L., and Tonegawa, S. (2017). Engrams and circuits crucial for systems consolidation of a memory. Science 356, 73-78.

Knobloch, H.S., Charlet, A., Hoffmann, L.C., Eliava, M., Khrulev, S., Cetin, A.H., Osten, P., Schwarz, M.K., Seeburg, P.H., Stoop, R., et al. (2012). Evoked axonal oxytocin release in the central amygdala attenuates fear response. Neuron 73, 553-566.

Koohi, M.K., Ivell, R., and Walther, N. (2005). Transcriptional activation of the oxytocin promoter by oestrogens uses a novel non-classical mechanism of oestrogen receptor action. J Neuroendocrinol 17, 197-207.

LeDoux, J. (2007). The amygdala. Curr Biol 17, R868-874.

Letzkus, J.J., Wolff, S.B., Meyer, E.M., Tovote, P., Courtin, J., Herry, C., and Luthi, A. (2011). A disinhibitory microcircuit for associative fear learning in the auditory cortex. Nature 480, 331-335.

Li, H., Penzo, M.A., Taniguchi, H., Kopec, C.D., Huang, Z.J., and Li, B. (2013). Experience-dependent modification of a central amygdala fear circuit. Nat Neurosci 16, 332-339.

Liu, X., Ramirez, S., Pang, P.T., Puryear, C.B., Govindarajan, A., Deisseroth, K., and Tonegawa, S.

(2012). Optogenetic stimulation of a hippocampal engram activates fear memory recall. Nature 484, 381-385.

Lutcke, H., Murayama, M., Hahn, T., Margolis, D.J., Astori, S., Zum Alten Borgloh, S.M., Gobel, W., Yang, Y., Tang, W., Kugler, S., et al. (2010). Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice. Front Neural Circuits 4, 9.

Martin, S.J., Grimwood, P.D., and Morris, R.G. (2000). Synaptic plasticity and memory: an evaluation of the hypothesis. Annu Rev Neurosci 23, 649-711.

Mobbs, D., Hagan, C.C., Dalgleish, T., Silston, B., and Prevost, C. (2015). The ecology of human fear:

survival optimization and the nervous system. Front Neurosci 9, 55.

Naumann, T., Hartig, W., and Frotscher, M. (2000). Retrograde tracing with Fluoro-Gold: different methods of tracer detection at the ultrastructural level and neurodegenerative changes of back-filled neurons in long-term studies. J Neurosci Methods 103, 11-21.

Penzo, M.A., Robert, V., Tucciarone, J., De Bundel, D., Wang, M., Van Aelst, L., Darvas, M., Parada, L.F., Palmiter, R.D., He, M., et al. (2015). The paraventricular thalamus controls a central amygdala fear circuit. Nature 519, 455-459.

Reijmers, L.G., Perkins, B.L., Matsuo, N., and Mayford, M. (2007). Localization of a stable neural correlate of associative memory. Science 317, 1230-1233.

Rhodes, C.H., Morrell, J.I., and Pfaff, D.W. (1981). Immunohistochemical analysis of magnocellular elements in rat hypothalamus: distribution and numbers of cells containing neurophysin, oxytocin, and vasopressin. J Comp Neurol 198, 45-64.

Ryan, T.J., Roy, D.S., Pignatelli, M., Arons, A., and Tonegawa, S. (2015). Memory. Engram cells retain memory under retrograde amnesia. Science 348, 1007-1013.

Schilling, K., Luk, D., Morgan, J.I., and Curran, T. (1991). Regulation of a fos-lacZ fusion gene: a paradigm for quantitative analysis of stimulus-transcription coupling. Proc Natl Acad Sci U S A 88, 5665-5669.

Somponpun, S.J., and Sladek, C.D. (2003). Osmotic regulation of estrogen receptor-beta in rat vasopressin and oxytocin neurons. J Neurosci 23, 4261-4269.

Stachniak, T.J., Ghosh, A., and Sternson, S.M. (2014). Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus-->midbrain pathway for feeding behavior. Neuron 82, 797-808.

Swanson, L.W., and Kuypers, H.G. (1980). The paraventricular nucleus of the hypothalamus:

cytoarchitectonic subdivisions and organization of projections to the pituitary, dorsal vagal complex, and spinal cord as demonstrated by retrograde fluorescence double-labeling methods. J Comp Neurol 194, 555-570.

Swanson, L.W., and Sawchenko, P.E. (1983). Hypothalamic integration: organization of the paraventricular and supraoptic nuclei. Annu Rev Neurosci 6, 269-324.

Theodosis, D.T., Montagnese, C., Rodriguez, F., Vincent, J.D., and Poulain, D.A. (1986). Oxytocin induces morphological plasticity in the adult hypothalamo-neurohypophysial system. Nature 322, 738-740.

Tobin, V., Leng, G., and Ludwig, M. (2012). The involvement of actin, calcium channels and exocytosis proteins in somato-dendritic oxytocin and vasopressin release. Front Physiol 3, 261.

Tovote, P., Fadok, J.P., and Luthi, A. (2015). Neuronal circuits for fear and anxiety. Nat Rev Neurosci 16, 317-331.

van den Pol, A.N. (2012). Neuropeptide transmission in brain circuits. Neuron 76, 98-115.

Viviani, D., Charlet, A., van den Burg, E., Robinet, C., Hurni, N., Abatis, M., Magara, F., and Stoop, R.

(2011). Oxytocin selectively gates fear responses through distinct outputs from the central amygdala. Science 333, 104-107.

Yang, H.P., Wang, L., Han, L., and Wang, S.C. (2013). Nonsocial functions of hypothalamic oxytocin.

ISRN Neurosci 2013, 179272.

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