Pseudomonas entomophila a été découverte dans l'étude du système immunitaire de la drosophile. En démontrant sa virulence envers plusieurs insectes, son caractère particulier entomopathogène a été révélé. Cette souche est très pathogène chez la drosophile car il tue rapidement la larve et l’adulte après ingestion. Il semble que Pe ait sélectionné des systèmes particuliers pour manifester sa virulence chez les insectes. Grâce à l'expérience de l'infection de la drosophile par le surnageant de culture de Pe, on a découvert que la métallo-protéine AprA est un facteur de virulence important. Cependant le mutant aprA peut persister dans l'intestin de la drosophile et provoque toujours une réponse immunitaire et une cessation d’ingestion de nourriture chez la drosophile. La bactérie Pe doit donc posséder d’autres facteurs de virulence qui lui permettent de persister et de provoquer des dommages à la drosophile. La synthèse de ces facteurs est supposée d’être contrôlée par le système Gac puisque la virulence de Pe est complètement abolie par des mutations du système Gac. Le but de ce projet était de disséquer le fonctionnement du système Gac de Pe, afin d’identifier des gènes régulés par ce système et qui sont en même temps impliqués dans la virulence surtout dans la persistance de Pe.
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